The ability to organize nano-components into the desired architectures with targeted properties can enable a broad range of nanotechnological applications, from energy materials to information processing. However, we are currently lacking an adaptable and broadly applicable methodology for the bottom-up 3D nanofabrication of desired nanoscale structures. I will discuss our efforts on establishing a versatile fabrication platform based on the molecularly programmable assembly for the formation of targeted architectures from inorganic and biomolecular nano-components. The recent advances on building periodic and hierarchical organizations, frameworks and arbitrary designed architectures using DNA-encoded interactions and DNA topologies will be presented. I will demonstrate how these assembly approaches can be used for a fabrication of nanomaterials with novel optical, mechanical, and catalytic functions.
We have fabricated a combined measurement system capable of confocal microscopy and fluorescence spectroscopy to
simultaneously evaluate multiple optical characteristics of single fluorescent nanocrystals. The single particle detection
sensitivity is demonstrated by simultaneously measuring the dynamic excitation-time-dependent fluorescence
intermittency and the emission spectrum of single cadmium selenide/zinc sulfide (CdSe/ZnS) nanocrystals (quantum
dots, QDs). Using this system, we are currently investigating the optical characteristics of single QDs, the surface of
which are conjugated with different ligands, such as trioctylphosphine oxide (TOPO), mercaptoundecanoicacid (MDA),
and amine modified DNA (AMDNA). In this paper, we present the progress of our measurements of the time-dependent
optical characteristics (fluorescence intermittency, photostability, and spectral diffusion) of single MDA-QDs and
AMDNA-MDA-QDs in air in an effort to understand the effects of surface-conjugated biomolecules on the optical
characteristics at single QD sensitivities.
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