Dr. Parameshwaran Diagaradjane Profile

Dr. Parameshwaran Diagaradjane

Research Scientist at Univ of Texas MD Anderson Cancer Ctr

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Publications (16)

SPIE Journal Paper | 1 March 2009 Open Access

Near-infrared narrow-band imaging of gold/silica nanoshells in tumors

Priyaveena Puvanakrishnan, Jaesook Park, Parameshwaran Diagaradjane, Jon Schwartz, Christopher Coleman, Kelly Gill-Sharp, Kristina Sang, J. Donald Payne, Sunil Krishnan, James Tunnell

JBO, Vol. 14, Issue 02, 024044, (March 2009) https://doi.org/10.1117/12.10.1117/1.3120494

KEYWORDS: Tumors, Near infrared, Tissues, Composites, Absorption, Gold, Visualization, Tissue optics, Particles, Blood

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Proceedings Article | 24 February 2009 Paper

Narrow band imaging of tumors using gold nanoshells

Priyaveena Puvanakrishnan, Jaesook Park, Parameshwaran Diagaradjane, Jon Schwartz, Chris Coleman, Kelly Gill-Sharp, Kristina Sang, J. Donald Payne, Sunil Krishnan, James Tunnell

Proceedings Volume 7169, 716912 (2009) https://doi.org/10.1117/12.809613

KEYWORDS: Tumors, Near infrared, Tissues, Gold, Absorption, Composites, Visualization, Tissue optics, Blood vessels, Blood

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Proceedings Article | 18 February 2009 Paper

Two-photon-induced photoluminescence imaging of gold nanoshell's tumor biodistribution

Jaesook Park, Arnold Estrada, Jon Schwartz, Parmeswaran Diagaradjane, Sunil Krishnan, Chris Coleman, J. Donald Payne, Andrew Dunn, James Tunnell

Proceedings Volume 7192, 71920T (2009) https://doi.org/10.1117/12.809620

KEYWORDS: Tumors, Satellite navigation systems, Luminescence, Gold, Tissues, Tissue optics, Cancer, Multiplexing, Particles, Microscopes

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Proceedings Article | 22 February 2008 Paper

EGF-conjugated near-infrared quantum dots as nanoprobes for in-vivo imaging of EGFR expression

Parmeswaran Diagaradjane, Jacobo Orenstein-Cardona, Norman Colón-Casasnovas, Amit Deorukhkar, Shujun Shentu, Norihito Kuno, David Schwartz, Juri Gelovani, Sunil Krishnan

Proceedings Volume 6866, 68660R (2008) https://doi.org/10.1117/12.763985

KEYWORDS: Nanoprobes, Tumors, Luminescence, Tissues, In vivo imaging, Quantum dots, Control systems, Nanoparticles, Confocal microscopy, Receptors

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Proceedings Article | 22 February 2008 Paper

Gold nanoshell mediated hyperthermia enhances the efficacy of radiation therapy

Parmeswaran Diagaradjane, Anil Shetty, James Wang, Andrew Elliot, Jon Schwartz, Shujun Shentu, Chul Park, Amit Deorukhkar, Jason Stafford, Sang Cho, James Tunnell, John Hazle, Sunil Krishnan

Proceedings Volume 6865, 68650N (2008) https://doi.org/10.1117/12.764021

KEYWORDS: Tumors, Gold, Tissues, Radiotherapy, Magnetic resonance imaging, Temperature metrology, Magnetism, Thermography, Laser irradiation, Thermometry

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SPIE Journal Paper | 1 July 2006 Open Access

Comparative study of cryogen spray cooling with R-134a and R-404a: implications for laser treatment of dark human skin

Tianhong Dai, Mohammad Yaseen, Parmeswaran Diagaradjane, David Chang, Bahman Anvari

JBO, Vol. 11, Issue 04, 041116, (July 2006) https://doi.org/10.1117/12.10.1117/1.2338001

KEYWORDS: Skin, Cryogenics, Injuries, Blood vessels, Temperature metrology, Ear, Laser irradiation, Laser therapeutics, In vivo imaging, In vitro testing

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Cutaneous laser treatment in dark skin patients is challenging due to significant light absorption by the melanin at the basal layer of epidermis, which can result in irreversible nonspecific thermal injury to the epidermis. Cryogen spray cooling (CSC) with R-134a (boiling point –26.2°C at 1 atm), which is currently used during cutaneous laser treatment, has shown poor efficacy in protecting dark human skin. We investigated the potential of CSC with R-404a (boiling point –46.5°C at 1 atm), which has a lower boiling point than R-134a, for improved therapeutic outcome in dark human skin at three levels: in vitro (epoxy resin skin phantom), ex vivo (normal dark human skin sample), and in vivo (skin of the rabbit external ear). The skin phantom was used to acquire the surface and internal temperature profiles in response to CSC with R-134a or R-404a at various spurt durations, based upon which CSC-induced heat removal from the skin phantom was estimated using an algorithm that solved a one-dimensional inverse heat conduction problem. CSC with R-404a increased the temperature reductions within the phantom and subsequently the amount of heat removal from the phantom in comparison to that with R-134a. Normal ex vivo Fitzpatrick types V-VI human skin samples were used to investigate the thermal response of dark human skin epidermis to CSC (R-134a or R-404a) at various spurt durations in conjunction with 595-nm pulsed dye laser irradiation at various radiant exposures. Cryogen R-404a increased the threshold radiant exposures for irreversible thermal injury to the epidermis in dark pigmentation skin. No obvious CSC-induced morphological changes to human skin was observed when sprayed with R404-a spurts using durations up to 300 ms. In vivo rabbit ear vasculature was used as a model of cutaneous anomalies to assess the influences of CSC (with R-134a or R-404a) on the photothermolysis of dermal blood vessels. CSC (R-134a or R-404a) with the spurt durations of 100 to 300 ms increased the most superficial depth of ...

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SPIE Journal Paper | 1 January 2006 Open Access

Synchronous fluorescence spectroscopic characterization of DMBA-TPA-induced squamous cell carcinoma in mice

Parmeswaran Diagaradjane, Mohammad Yaseen, Jie Yu, Michael Wong, Bahman Anvari

JBO, Vol. 11, Issue 01, 014012, (January 2006) https://doi.org/10.1117/12.10.1117/1.2167933

KEYWORDS: Tissues, Tumors, Skin, Luminescence, Statistical analysis, Fluorescence spectroscopy, Spectroscopy, Collagen, Diagnostics, Biological research

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Proceedings Article | 25 April 2005 Paper

Autofluorescence characterization of DMBA-TPA-induced two-stage carcinogenesis in mouse skin for the early detection of tissue transformation

Parmeswaran Diagaradjane, Mohammed Yaseen, Jie Yu, Michael Wong, Bahman Anvari

Proceedings Volume 5686, (2005) https://doi.org/10.1117/12.589328

KEYWORDS: Tissues, Skin, Luminescence, Tumors, Statistical analysis, Collagen, Diagnostics, Tissue optics, Fluorescence spectroscopy, Cancer

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The use of autofluorescence technique in the characterization of the sequential tissue transformation process in 7,12-dimethylbenz(a)anthracene and 12-O-tetradecanoylphorbol-13-acetate (DMBA & TPA) induced two-stage mouse skin carcinogenesis model in conjunction with a suitable statistical method is being explored. The fluorescence excitation emission matrix (EEM) from experimental group (n=40; DMBA/TPA application), control group (n=6; acetone application) and the blank group (n=6; no application of DMBA/TPA or acetone) were measured every week using Fluoromax3 spectrofluorometer coupled with a waveguide fiber optic bundle (JY Horiba, NJ). The EEM was recorded for 19 excitation wavelengths from 280 to 460 nm at 10 nm intervals and the fluorescence emission was scanned from 300 to 750 nm. During the tissue transformation the epithelial tissues underwent biochemical and structural changes that are manifested in the tissue fluorescence. To correlate the tissue morphology with the observed fluorescence differences in the fluorescence emission, animals were sacrificed and the tissue biopsies were subjected to histopathological evaluation. The fluorescence emission corresponding to different fluorophores was extracted from the EEM, and the spectral data were used in multivariate statistical algorithm for the earliest diagnosis of the onset of tissue transformation. The intrinsic fluorescence from tryptophan, NADH and prophyrins showed distinct differences in the spectral signature during the tissue transformation, due to the altered metabolic activities of the cells. The statistical analysis of the spectral data corresponding to each excitation wavelength showed better classification accuracy at 280, 320, 350 and 405 nm excitations, corresponding to tryptophan, collagen, NADH and porphyrins with the classification accuracy of 74.3, 68.1, 64.6 and 74.7 % respectively. The variations in the spectral signature and the results of the statistical analysis suggest that porphyrins, tryptophan and NADH can be targeted as potential tumor markers in the early detection of the tissue transformation process.

Proceedings Article | 15 April 2005 Paper

Photothermal and photochemical effects of laser light absorption by indocyanine green (ICG)

Mohammad Yaseen, Parmeswaran Diagaradjane, Brian Pikkula, Jie Yu, Michael Wong, Bahman Anvari

Proceedings Volume 5695, (2005) https://doi.org/10.1117/12.589702

KEYWORDS: Photodynamic therapy, Blood vessels, Absorption, Ear, Tissues, Photothermal effect, Indocyanine green, Collagen, Chromophores, Pulsed laser operation

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Indocyanine Green (ICG) is clinically used as a fluorescent dye for imaging purposes. Its rapid circulation kinetics and minimal toxicity has prompted investigation into ICG's utility as a photosentitizer for therapeutic applications. Traditionally, optically mediated tumor therapy has focused on photodynamic therapy, which employs a photochemical mechanism resulting from the absorption of low intensity CW laser light by localized photosensitizers such as Photofrin II, Benzoporphyrin Derivative (BPD), ICG. Treatment of cutaneous vascular malformations such as port-wine stains, on the other hand, is based on a photothermal mechanism resulting from the absorption of high intensity pulsed laser light by hemoglobin. In this study, we compared the effectiveness of combining photochemical and photothermal mechanisms during application of ICG in conjunction with laser irradiation with the intention that the combined approach may lead to a reduction in the threshold dose of pulsed laser light required to treat hypervascular malformations. The blood vessels in rabbit ears were used as an in vivo model for targeted vasculature. Irradiation of the ears with IR light (λ=785 nm, Δτ = 3 min, I_o = 120 mW) was used to elicit photochemical damage, while photothermal damage was brought about using pulses from a ruby laser (λ=694 nm, τ = 3 ms) with different fluences. For the combined modality, photochemical damage was induced first and followed by photothermal irradiation. This modality was compared with photothermal irradiation alone. The effectiveness of each irradiation scheme was assessed using histopathological analysis. We present preliminary data that suggests that pretreatment with photodynamic therapy before photothermal coagulation results in more severe vascular damage with lower photothermal fluence levels. The results of this study provide the foundation work for further exploration of the therapeutic potentials of photochemical and photothermal effects during application of ICG in conjunction with laser irradiation.

Proceedings Article | 26 October 2001 Paper

In-vivo pharmacokinetics of delta-ALA induced PPIX during photodynamic therapy on mice tumor model

Diagaradjane Parmeswearan, Prakasa Aruna, Pradeep Gupta, Singaravelu Ganesan

Proceedings Volume 4433, (2001) https://doi.org/10.1117/12.446514

KEYWORDS: Photodynamic therapy, Tumors, Tissues, Luminescence, Mathematical modeling, In vivo imaging, Diffusion, Data modeling, Laser therapeutics, Cancer

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In the recent years (delta) -aminolevulinic acid ((delta) -ALA) a precursor for the endogenous production of protoporphyrin IX (PPIX) has gained importance in the Photodynamic Therapy (PDT) of superficial and early-stage cancers. Though (delta) -ALA is present naturally in the cells, systemic administration of exogenous (delta) -ALA leads to the production of intracellular endogenous PP IX in both the tumor and the normal cells, but with varying concentration. However, the PPIX is accumulated more in the tumor tissues as the fast growing tumor cells take up the administered (delta) -ALA more than the normal cells. As the therapeutic efficacy of PDT is dependent on the post (delta) -ALA incubation time, at which the tumor to normal ratio of the PPIX concentration is high, the concentration of the PPIX in the normal and the tumor site were estimated using fluorescence spectroscopy. However, the estimation of the PPIX concentration during/after PDT is mandatory, as the PDT dosimetry is dependent on the sensitizer concentration at the target of interest. The observed variation in the concentration of PPIX in the tumor site with respect to the unexposed normal surrounding tissues, may be attributed to the diffusion of PPIX from the surrounding normal tissues to the tumor site, across the concentration gradient. Based on this a mathematical model has been proposed, to estimate the rate parameter for the diffusion of PPIX from the surrounding normal tissues in to the tumor tissue (K_m), due to photobleaching during PDT at two different fluence. The K_m value at two different fluences, 57.6 and 36 J/cm2 are estimated as 5.444+/- 1.186 and 3.221+/- 0.957, respectively. Further, the rate parameter for the cleavage and efflux of (delta) -ALA (K₁), and the rate parameter for the evasion of the PPIX from the tumor tissues during PDT (K_t), were also estimated by fitting the experimental data to the developed mathematical model. The estimated parameters will be utilized to estimate the exact concentration of PPIX in the tumor tissues for a better PDT efficacy.

Proceedings Article | 16 April 2001 Paper

Optical biopsy of normal and cancerous cervical tissues by native fluorescence spectroscopy

Nammalver Vengadesan, K. Muthuvelu, Thalaimuthu Anbupalam, S. Madhuri, Diagaradjane Parmeswearan, Prakasa Aruna, Singaravelu Ganesan

Proceedings Volume 4261, (2001) https://doi.org/10.1117/12.424530

KEYWORDS: Tissues, Statistical analysis, Luminescence, Fluorescence spectroscopy, Cancer, Cervical cancer, Biopsy, Tissue optics, Collagen, Principal component analysis

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Proceedings Article | 29 March 2000 Paper

Comparison of PDT efficiencies for photo-oxidation of substrate (NADPH) using a photosensitizer (Photofrin II)

Paulo Bargo, Parmeswararn Diagaradjane, Steven Jacques

Proceedings Volume 3909, (2000) https://doi.org/10.1117/12.379887

KEYWORDS: Oxygen, Diffusion, Absorbance, Quantum efficiency, Photodynamic therapy, Molecules, In vivo imaging, Molecular interactions, Systems modeling, Oxidation

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Proceedings Article | 6 July 1999 Paper

Photodynamic activity on human erythrocytes by a newly synthesized sensitizer--trithia sapphyrin sulfonate: a preliminary report

Diagaradjane Parmeswearan, Singaravelu Ganesan, K. Thanigaivelu, Simi Pushpan, A. Srinivasan, M. Ravi Kumar, T. Chandrashekar

Proceedings Volume 3592, (1999) https://doi.org/10.1117/12.351511

KEYWORDS: Absorption, Molecules, Photodynamic therapy, Environmental sensing, Luminescence, Tissues, Oxygen, Sulfur, Nitrogen, Diffusion

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Proceedings Article | 19 May 1999 Paper

Comparison of bond strength and surface morphology of dental enamel for acid and Nd-YAG laser etching

Diagaradjane Parmeswearan, Singaravelu Ganesan, P. Ratna, D. Koteeswaran

Proceedings Volume 3593, (1999) https://doi.org/10.1117/12.348332

KEYWORDS: Teeth, Laser bonding, Etching, Laser dentistry, Nd:YAG lasers, Scanning electron microscopy, Photography, Laser energy, Interfaces, Adhesives

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Proceedings Article | 17 May 1999 Paper

Native fluorescence characteristics of blood plasma during rat liver regeneration

Singaravelu Ganesan, S. Madhuri, V. Anuradha, A. Namasivayam, Diagaradjane Parmeswearan

Proceedings Volume 3599, (1999) https://doi.org/10.1117/12.348376

KEYWORDS: Luminescence, Blood, Plasma, Liver, Tissues, Tissue optics, Visible radiation, Injuries, Cancer, Animal model studies

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Proceedings Article | 18 August 1997 Paper

Native cellular fluorescence characteristics of normal and malignant epithelial cells from human larynx

Diagaradjane Parmeswearan, Singaravelu Ganesan, R. Nalini, Prakasa Aruna, V. Veeraganesh, Robert Alfano

Proceedings Volume 2979, (1997) https://doi.org/10.1117/12.280222

KEYWORDS: Luminescence, Tissues, Fluorescence spectroscopy, Diagnostics, Oncology, Breast, Ultraviolet radiation, Tissue optics, Tumors, Colon

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Showing 5 of 16 publications

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