Dr. Prarthana Shrestha Profile

Dr. Prarthana Shrestha

at Philips Medical Systems International BV

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SPIE Journal Paper | 14 July 2014 Open Access

Color accuracy and reproducibility in whole slide imaging scanners

Prarthana Shrestha, Bas Hulsken

JMI, Vol. 1, Issue 02, 027501, (July 2014) https://doi.org/10.1117/12.10.1117/1.JMI.1.2.027501

KEYWORDS: Scanners, Calibration, Tissues, Color reproduction, Color difference, Visualization, RGB color model, Pathology, Profiling, Manufacturing

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We propose a workflow for color reproduction in whole slide imaging (WSI) scanners, such that the colors in the scanned images match to the actual slide color and the inter-scanner variation is minimum. We describe a new method of preparation and verification of the color phantom slide, consisting of a standard IT8-target transmissive film, which is used in color calibrating and profiling the WSI scanner. We explore several International Color Consortium (ICC) compliant techniques in color calibration/profiling and rendering intents for translating the scanner specific colors to the standard display (sRGB) color space. Based on the quality of the color reproduction in histopathology slides, we propose the matrix-based calibration/profiling and absolute colorimetric rendering approach. The main advantage of the proposed workflow is that it is compliant to the ICC standard, applicable to color management systems in different platforms, and involves no external color measurement devices. We quantify color difference using the CIE-DeltaE2000 metric, where DeltaE values below 1 are considered imperceptible. Our evaluation on 14 phantom slides, manufactured according to the proposed method, shows an average inter-slide color difference below 1 DeltaE. The proposed workflow is implemented and evaluated in 35 WSI scanners developed at Philips, called the Ultra Fast Scanners (UFS). The color accuracy, measured as DeltaE between the scanner reproduced colors and the reference colorimetric values of the phantom patches, is improved on average to 3.5 DeltaE in calibrated scanners from 10 DeltaE in uncalibrated scanners. The average inter-scanner color difference is found to be 1.2 DeltaE. The improvement in color performance upon using the proposed method is apparent with the visual color quality of the tissue scans.

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Proceedings Article | 20 March 2014 Paper

Color accuracy and reproducibility in whole slide imaging scanners

Prarthana Shrestha, Bas Hulsken

Proceedings Volume 9041, 904112 (2014) https://doi.org/10.1117/12.2048769

KEYWORDS: Scanners, Calibration, Color reproduction, Visualization, Tissues, RGB color model, Glasses, Color management, Manufacturing, Microscopes

Read Abstract +

In this paper, we propose a work-flow for color reproduction in whole slide imaging (WSI) scanners such that the colors in the scanned images match to the actual slide color and the inter scanner variation is minimum. We describe a novel method of preparation and verification of the color phantom slide, consisting of a standard IT8- target transmissive film, which is used in color calibrating and profiling the WSI scanner. We explore several ICC compliant techniques in color calibration/profiling and rendering intents for translating the scanner specific colors to the standard display (sRGB) color-space. Based on the quality of color reproduction in histopathology tissue slides, we propose the matrix-based calibration/profiling and absolute colorimetric rendering approach. The main advantage of the proposed work-ow is that it is compliant to the ICC standard, applicable to color management systems in different platforms, and involves no external color measurement devices. We measure objective color performance using CIE-DeltaE2000 metric, where DeltaE values below 1 is considered imperceptible. Our evaluation 14 phantom slides, manufactured according to the proposed method, show an average inter-slide color difference below 1 DeltaE. The proposed work-flow is implemented and evaluated in 35 Philips Ultra Fast Scanners (UFS). The results show that the average color difference between a scanner and the reference is 3.5 DeltaE, and among the scanners is 3.1 DeltaE. The improvement on color performance upon using the proposed method is apparent on the visual color quality of the tissues scans.

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