Dr. Qi Pian Profile

Dr. Qi Pian

Research Fellow at Athinoula A Martinos Ctr for Biomedical Imaging

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Publications (9)

Proceedings Article | 13 March 2024 Presentation

Capillary blood flow imaging in awake mice using dynamic light scattering OCT and two photon microscopy

Sida Liang, Qi Pian, Mohammed Alfadhel, Buyin Fu, Jianbo Tang, Sava Sakadzic

Proceedings Volume PC12841, PC128410F (2024) https://doi.org/10.1117/12.3023450

KEYWORDS: Blood circulation, Capillaries, Two photon excitation microscopy, Optical coherence tomography, Dynamic light scattering, Biological imaging, Animal model studies, Red blood cells, Animals, Velocimetry

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SPIE Journal Paper | 21 July 2023 Open Access

Cortical microvascular blood flow velocity mapping by combining dynamic light scattering optical coherence tomography and two-photon microscopy

Qi Pian, Mohammed Alfadhel, Jianbo Tang, Grace V. Lee, Baoqiang Li, Buyin Fu, Yagmur Ayata, Mohammad Abbas Yaseen, David A. Boas, Timothy W. Secomb, Sava Sakadzic

JBO, Vol. 28, Issue 07, 076003, (July 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.7.076003

KEYWORDS: Angiography, Optical coherence tomography, Capillaries, Blood circulation, Image segmentation, Brain, Biological imaging, Data acquisition, Voxels, Velocity measurements

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SignificanceThe accurate large-scale mapping of cerebral microvascular blood flow velocity is crucial for a better understanding of cerebral blood flow (CBF) regulation. Although optical imaging techniques enable both high-resolution microvascular angiography and fast absolute CBF velocity measurements in the mouse cortex, they usually require different imaging techniques with independent system configurations to maximize their performances. Consequently, it is still a challenge to accurately combine functional and morphological measurements to co-register CBF speed distribution from hundreds of microvessels with high-resolution microvascular angiograms.AimWe propose a data acquisition and processing framework to co-register a large set of microvascular blood flow velocity measurements from dynamic light scattering optical coherence tomography (DLS-OCT) with the corresponding microvascular angiogram obtained using two-photon microscopy (2PM).ApproachWe used DLS-OCT to first rapidly acquire a large set of microvascular velocities through a sealed cranial window in mice and then to acquire high-resolution microvascular angiograms using 2PM. The acquired data were processed in three steps: (i) 2PM angiogram coregistration with the DLS-OCT angiogram, (ii) 2PM angiogram segmentation and graphing, and (iii) mapping of the CBF velocities to the graph representation of the 2PM angiogram.ResultsWe implemented the developed framework on the three datasets acquired from the mice cortices to facilitate the coregistration of the large sets of DLS-OCT flow velocity measurements with 2PM angiograms. We retrieved the distributions of red blood cell velocities in arterioles, venules, and capillaries as a function of the branching order from precapillary arterioles and postcapillary venules from more than 1000 microvascular segments.ConclusionsThe proposed framework may serve as a useful tool for quantitative analysis of large microvascular datasets obtained by OCT and 2PM in studies involving normal brain functioning, progression of various diseases, and numerical modeling of the oxygen advection and diffusion in the realistic microvascular networks.

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Proceedings Article | 28 April 2022 Presentation

Effects of voluntary exercise on cerebral microvascular oxygenation in aged mice

Paul Shin, Qi Pian, Hidehiro Ishikawa, Gen Hamanaka, Emiri Mandeville, Guo Shuzhen, Buyin Fu, Mohammed Alfadhel, Ikbal Sencan, Baoqiang Li, Chongzhao Ran, Sergei Vinogradov, Eng Lo, Ken Arai, Anna Devor, Sava Sakadžic

Proceedings Volume PC11946, PC119460S (2022) https://doi.org/10.1117/12.2610303

KEYWORDS: Capillaries, Two photon excitation microscopy, Oxygen, Brain

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Proceedings Article | 7 March 2022 Poster

Background signal rejection for pO2 measurements using adaptive optics and two-photon phosphorescence lifetime microscopy

Qi Pian, Baoqiang Li, Ikbal Sencan, Xiaojun Cheng, Jay Dubb, Xinyue Huang, Buyin Fu, Sergei Vinogradov, David Boas, Sava Sakadžic

Proceedings Volume 11946, 1194605 (2022) https://doi.org/10.1117/12.2609110

KEYWORDS: Phosphorescence, Microscopy, Adaptive optics, Optical testing, Oxygen, Tissues, Spatial light modulators, Brain, Temporal resolution, Quantum efficiency

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Proceedings Article | 5 March 2021 Presentation

Large scale mapping of the cortical microvascular velocities by the dynamic light scattering optical coherence tomography

Mohammed Alfadhel, Qi Pian, Jianbo Tang, Grace Lee, Baoqiang Li, Buyin Fu, Yagmur Ayata, David Boas, Timothy Secomb, Sava Sakadžic

Proceedings Volume 11629, 116290Z (2021) https://doi.org/10.1117/12.2583146

KEYWORDS: Optical coherence tomography, Dynamic light scattering, Blood circulation, Oxygen, Angiography, Two photon excitation microscopy, Quantitative analysis, Numerical modeling, Image segmentation, Diffusion

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Proceedings Article | 5 March 2021 Poster

Two-photon microscopic imaging of the circadian rhythm induced changes in the cerebral microvascular blood flow

Baoqiang Li, Ikbal Sencan, Ji Leng, Ryo Ohtomo, Hajime Takase, Jason Porter, Qi Pian, Martin Thunemann, Stephen Adams, Jing Yang, Buyin Fu, Mohammad Yaseen, Chongzhao Ran, David Boas, Anna Devor, Ken Arai, Cenk Ayata, Eng Lo, Sava Sakadžic

Proceedings Volume 11629, 116292O (2021) https://doi.org/10.1117/12.2579395

KEYWORDS: Blood circulation, Two photon imaging, Capillaries, Neuroprotection, Ischemia, Cranial windows, Cerebral blood flow, Brain

Proceedings Article | 21 October 2016 Paper

Innovation and fusion of x-ray and optical tomography for mouse studies of breast cancer

Ge Wang, Wenxiang Cong, Qingsong Yang, Qi Pian, Shouping Zhu, Jimin Liang, Margarida Barroso, Xavier Intes

Proceedings Volume 9967, 99671R (2016) https://doi.org/10.1117/12.2238188

KEYWORDS: X-rays, Imaging systems, X-ray optics, Optical tomography, Breast cancer, X-ray imaging, Tomography, Tissue optics, Receptors, Tumors

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Proceedings Article | 10 March 2016 Paper

Time-resolved hyperspectral single-pixel camera implementation for compressive wide-field fluorescence lifetime imaging

Qi Pian, Ruoyang Yao, Xavier Intes

Proceedings Volume 9701, 970115 (2016) https://doi.org/10.1117/12.2217686

KEYWORDS: Data acquisition, Digital micromirror devices, Cameras, Signal to noise ratio, Image quality, Liver, Image restoration, Modulation, Luminescence, Fluorescence lifetime imaging

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Proceedings Article | 17 February 2014 Paper

Structured light based hyperspectral time-resolved diffuse optical tomography system

Qi Pian, Xavier Intes

Proceedings Volume 8937, 89370T (2014) https://doi.org/10.1117/12.2038546

KEYWORDS: Data acquisition, Absorption, Digital Light Processing, Imaging systems, Monte Carlo methods, Diffuse optical tomography, Luminescence, Scattering, Digital micromirror devices, Structured light

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