The progression of obesity can be influenced by lipid metabolism and alterations in fatty acid levels. This study utilized Raman techniques to analyze the impact of High-Fat Diet (HFD) consumption on White Adipose Tissue (WAT) in an animal model (mice). Our results from statistical examination of Raman spectra indicated a substantial increase in unsaturated lipid levels in Visceral WAT (VWAT) fat pads when exposed to a high-fat die. The VWAT tissues were analyzed and mapped using a targeted Raman image analysis method employing Direct Classical Least Squares (DCLS) approaches to characterize lipid species such as ω-3 and ω-6 fatty acids. The analysis showed higher concentrations of ω3, ω6, cholesterol, and triglycerides in adipose tissues from the high-fat diet group compared to the Low-Fat Diet (LFD) group. The study demonstrated that Raman spectroscopy and microscopy, as a reliable and non-invasive technique, offered important understanding at the molecular level into the process of lipid species remodeling and the spatial distribution of adipose tissues during a high-fat diet.
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