We built a fibre optic illuminated deep ultraviolet (DUV) fluorescence microscope. Our microscope was designed to be compact and portable, sitting on a 30cm by 30cm breadboard. Multimode fibres were magnetically butt coupled to 2 DUV light emitting diodes, which provided oblique epi-illumination of biological samples with short working distance objectives. The stage was motorised by stepper motors and controlled by a microcontroller which allowed for high-resolution imaging of large, fresh, and unsectioned tissue with high-throughput. We demonstrated the fluorescence microscope across a wide range of biological samples from single cells and spheroids to large tumours and organ samples.
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