Mr. Rainer Erdmann Profile

Rainer Erdmann

Managing Director at PicoQuant GmbH

SPIE Involvement:

Conference Chair | Editor | Author

Publications (62)

Proceedings Article | 5 March 2021 Presentation

Measuring steady-state and time-resolved photoluminescence from a positionable, micrometer-sized observation volume

Christian Oelsner, Eugeny Ermilov, Volker Buschmann, Frank Birke, Matthias Patting, Uwe Ortmann, Rainer Erdmann

Proc. SPIE. 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021

KEYWORDS: Semiconductors, Proteins, Statistical analysis, Spectroscopy, Luminescence, Molecules, Resonance energy transfer, Molecular spectroscopy, Time resolved spectroscopy, Spatial resolution

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Proceedings Article | 5 March 2021 Presentation

Visualizing dynamic processes with rapidFLIMHiRes, the ultra fast FLIM imaging method with outstanding 10ps time resolution

Maria Loidolt-Krüger, Fabian Jolmes, Matthias Patting, Michael Wahl, Evangelos Sismakis, André Devaux, Uwe Ortmann, Felix Koberling, Rainer Erdmann

Proc. SPIE. 11648, Multiphoton Microscopy in the Biomedical Sciences XXI

KEYWORDS: Visualization, Tissues, Sensors, Single photon, Image resolution, Temporal resolution, Fluorescence lifetime imaging, Picosecond phenomena, Fluorescence resonance energy transfer, Ultrafast imaging

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Proceedings Article | 5 March 2021 Presentation

Visualizing dynamic processes with rapidFLIMHiRes, the ultra fast FLIM imaging method with outstanding 10ps time resolution

Maria Loidolt-Krüger, Fabian Jolmes, Matthias Patting, Michael Wahl, Evangelos Sismakis, André Devaux, Uwe Ortmann, Felix Koberling, Rainer Erdmann

Proc. SPIE. 11654, High-Speed Biomedical Imaging and Spectroscopy VI

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Proceedings Article | 5 March 2021 Presentation

Welcome and Introduction to SPIE Conference: 11650

Rainer Erdmann, Ingo Gregor, Felix Koberling

Proc. SPIE. 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV

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Proceedings Article | 26 March 2020 Presentation

Scanning FCS and super-resolution microscopy on 2D lipid membranes (Conference Presentation)

Uwe Ortmann, Mariano Gonzalez Pisfil, Marcelle König, Rhys Dowler, Benedikt Krämer, Sumeet Rohilla, Felix Koberling, Rainer Erdmann

Proc. SPIE. 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII

KEYWORDS: Confocal microscopy, Microscopes, Calibration, Molecules, Diffusion, Super resolution microscopy, Time resolved spectroscopy, Fluorescence correlation spectroscopy, Stimulated emission depletion microscopy, Cell mechanics

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Proceedings Article | 9 March 2020 Presentation

Multi-target immunofluorescence using spectral FLIM-FRET for separation of undesirable antibody cross-labeling and autofluorescence (Conference Presentation)

Sumeet Rohilla, Benedikt Kraemer, Felix Koberling, Uwe Ortmann, Ingo Gregor, Andreas Hocke, Rainer Erdmann

Proc. SPIE. 11244, Multiphoton Microscopy in the Biomedical Sciences XX

KEYWORDS: Electronics, Tissues, Luminescence, Molecules, Lung, Fluorescence lifetime imaging, Time resolved spectroscopy, Fluorescence resonance energy transfer, Molecular interactions, Data analysis

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Proceedings Article | 21 February 2020 Paper

Programmable, pulse shaped diode laser

Paul Fey, Thomas Schönau, Kristian Lauritsen, Rainer Erdmann

Proc. SPIE. 11259, Solid State Lasers XXIX: Technology and Devices

KEYWORDS: Logic, Clocks, Modulation, Semiconductor lasers, Pulse shaping, Picosecond phenomena, Analog electronics

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SPIE Journal Paper | 10 May 2019

BabyLux device: a diffuse optical system integrating diffuse correlation spectroscopy and time-resolved near-infrared spectroscopy for the neuromonitoring of the premature newborn brain

Martina Giovannella, Davide Contini, Marco Pagliazzi, Antonio Pifferi, Lorenzo Spinelli, Rainer Erdmann, Roger Donat, Ignacio Rocchetti, Matthias Rehberger, Niels König, Robert Schmitt, Alessandro Torricelli, Turgut Durduran, Udo Weigel

NPh Vol. 6 Issue 02

KEYWORDS: Optical properties, Near infrared spectroscopy, Sensors, Solids, Diffusion, Tissues, Spectroscopes, Liquids, Tissue optics, Neurophotonics

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Proceedings Article | 4 March 2019 Presentation

Mapping molecules quantitatively in confocal fluorescence microscopy (Conference Presentation)

Marcelle Koenig, Caroline Berlage, Paja Reisch, Felix Koberling, Uwe Ortmann, Haisen Ta, Rainer Erdmann

Proc. SPIE. 10884, Single Molecule Spectroscopy and Superresolution Imaging XII

KEYWORDS: Confocal microscopy, Statistical analysis, Microscopy, Luminescence, Molecules, Single photon, Associative arrays, Molecular lasers, Tantalum, Systems modeling

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Proceedings Article | 4 March 2019 Presentation

Quantitative ultra-fast FLIM and lifetime based multi-species analysis (Conference Presentation)

Benedikt Kraemer, Sumeet Rohilla, Rhys Dowler, Marcus Sackrow, Matthias Patting, Hans-Jürgen Rahn, Michael Wahl, Uwe Ortmann, Felix Koberling, Andreas Hocke, Rainer Erdmann

Proc. SPIE. 10882, Multiphoton Microscopy in the Biomedical Sciences XIX

KEYWORDS: Confocal microscopy, Ultrafast phenomena, Tissues, Sensors, Image processing, Luminescence, Photomultipliers, Life sciences, Fluorescence lifetime imaging, Spatial resolution

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Proceedings Article | 4 March 2019 Presentation

Spectrally resolved and high speed TCSPC-based fluorescence lifetime imaging (Conference Presentation)

Tino Roehlicke, Matthias Patting, Hans-Juergen Rahn, Benedikt Kraemer, Michael Wahl, Felix Koberling, Rainer Erdmann

Proc. SPIE. 10889, High-Speed Biomedical Imaging and Spectroscopy IV

KEYWORDS: Confocal microscopy, Electronics, Sensors, Luminescence, Single photon, Image acquisition, Live cell imaging, Fluorescence lifetime imaging, Picosecond phenomena, Signal detection

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Proceedings Article | 24 April 2017 Presentation

Rapid FLIM: The new and innovative method for ultra-fast imaging of biological processes (Conference Presentation)

Sandra Orthaus-Mueller, Benedikt Kraemer, Astrid Tannert, Tino Roehlicke, Michael Wahl, Hans-Juergen Rahn, Felix Koberling, Rainer Erdmann

Proc. SPIE. 10069, Multiphoton Microscopy in the Biomedical Sciences XVII

KEYWORDS: Ultrafast phenomena, Sensors, Image processing, Photons, Single photon, Data acquisition, Signal processing, Fluorescence lifetime imaging, Time resolved spectroscopy, Fluorescence resonance energy transfer

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Proceedings Article | 20 February 2017 Paper

Picosecond pulsed micro-module emitting near 560 nm using a frequency doubled gain-switched DBR ridge waveguide semiconductor laser

André Kaltenbach, Julian Hofmann, Dirk Seidel, Kristian Lauritsen, Frank Bugge, Jörg Fricke, Katrin Paschke, Rainer Erdmann, Günther Tränkle

Proc. SPIE. 10088, Nonlinear Frequency Generation and Conversion: Materials and Devices XVI

KEYWORDS: Laser sources, Second-harmonic generation, Waveguides, Fluorescence spectroscopy, Semiconductor lasers, Distributed Bragg reflectors, Picosecond lasers, Picosecond phenomena, Harmonic generation, Frequency conversion, Nonlinear crystals, Waveguide lasers, Pulsed laser operation

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Proceedings Article | 14 March 2016 Paper

ns-time resolution for multispecies STED-FLIM and artifact free STED-FCS

Marcelle Koenig, Paja Reisch, Rhys Dowler, Benedikt Kraemer, Sebastian Tannert, Matthias Patting, Mathias Clausen, Silvia Galiani, Christian Eggeling, Felix Koberling, Rainer Erdmann

Proc. SPIE. 9712, Multiphoton Microscopy in the Biomedical Sciences XVI

KEYWORDS: Confocal microscopy, Diffraction, Super resolution, Microscopy, Luminescence, Molecules, Diffusion, Single photon, Time correlated photon counting, Optical resolution, Picosecond phenomena, Fluorescence correlation spectroscopy, Pulsed laser operation, Stimulated emission depletion microscopy

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Proceedings Article | 27 February 2015 Paper

Generation of third harmonic picosecond pulses at 355 nm by sum frequency mixing in periodically poled MgSLT crystal

André Kaltenbach, Thomas Schönau, Kristian Lauritsen, Günther Tränkle, Rainer Erdmann

Proc. SPIE. 9347, Nonlinear Frequency Generation and Conversion: Materials, Devices, and Applications XIV

KEYWORDS: Fiber amplifiers, Magnesium, Ultraviolet radiation, Crystals, Semiconductor lasers, Picosecond phenomena, Harmonic generation, Frequency conversion, Nonlinear crystals, Laser systems engineering

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Proceedings Article | 12 March 2013 Paper

Pulsed picosecond 766 nm laser source operating between 1-80 MHz with automatic pump power management

Thomas Schönau, Torsten Siebert, Romano Härtel, Thomas Eckhardt, Dietmar Klemme, Kristian Lauritsen, Rainer Erdmann

Proc. SPIE. 8604, Nonlinear Frequency Generation and Conversion: Materials, Devices, and Applications XII

KEYWORDS: Fiber amplifiers, Laser sources, Lithium niobate, Second-harmonic generation, Optical amplifiers, Crystals, Semiconductor lasers, Picosecond phenomena, Frequency conversion, Stimulated emission depletion microscopy

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Proceedings Article | 26 February 2013 Paper

Picosecond supercontinuum laser with consistent emission parameters over variable repetition rates from 1 to 40 MHz

Thomas Schönau, Torsten Siebert, Romano Härtel, Dietmar Klemme, Kristian Lauritsen, Rainer Erdmann

Proc. SPIE. 8601, Fiber Lasers X: Technology, Systems, and Applications

KEYWORDS: Fiber amplifiers, Visible radiation, Optical amplifiers, Phase modulation, Spectroscopy, Luminescence, Semiconductor lasers, Diodes, Microcontrollers, Picosecond phenomena

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Proceedings Article | 10 October 2012 Paper

Characterization and quality control of semiconductor wafers using time-correlated single photon counting

V. Buschmann, S. Fore, F. Koberling, A. Knigge, P. Kapusta, U. Ortmann, M. Roczen, R. Erdmann

Proc. SPIE. 8470, Thin Film Solar Technology IV

KEYWORDS: Quantum wells, Sensors, Luminescence, Gallium arsenide, Semiconductor materials, Single photon, Semiconductor lasers, Picosecond phenomena, Semiconducting wafers, Pulsed laser operation

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Proceedings Article | 15 February 2012 Paper

Picosecond pulse generation at 596 nm via sum-frequency mixing with a gain-switched, fiber-amplified laser diode

Kristian Lauritsen, Thomas Schönau, Torsten Siebert, Rainer Erdmann

Proc. SPIE. 8240, Nonlinear Frequency Generation and Conversion: Materials, Devices, and Applications XI

KEYWORDS: Laser sources, Ferroelectric materials, Optical amplifiers, Waveguides, Polarization, Wavelength division multiplexing, Semiconductor lasers, Diodes, Picosecond phenomena, Sum-frequency generation

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Proceedings Article | 3 February 2012 Paper

Time-resolved single molecule microscopy coupled with atomic force microscopy

M. König, F. Koberling, O. Schulz, R. Ros, S. Fore, M. Sackrow, S. Trautmann, R. Erdmann

Proc. SPIE. 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX

KEYWORDS: Confocal microscopy, Microscopes, Green fluorescent protein, Imaging systems, Microscopy, Luminescence, Molecules, Atomic force microscopy, Data acquisition, Fluorescence lifetime imaging

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Proceedings Article | 14 May 2011 Paper

Tau-SPAD: a new red sensitive single-photon counting module

Gerald Kell, Andreas Bülter, Michael Wahl, Rainer Erdmann

Proc. SPIE. 8033, Advanced Photon Counting Techniques V

KEYWORDS: Photodetectors, Photon counting, Electronics, Sensors, Molecules, Single photon, Picosecond phenomena, Avalanche photodiodes, Fluorescence correlation spectroscopy, Quenching (fluorescence)

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Proceedings Article | 23 February 2011 Paper

Buffer controlled photoswitching microscopy using standard organic fluorophores

Volker Buschmann, Samantha Fore, Sebastian van de Linde, Markus Sauer, Steve Wolter, Mike Heilemann, Felix Koberling, Rainer Erdmann

Proc. SPIE. 7905, Single Molecule Spectroscopy and Imaging IV

KEYWORDS: Microscopes, Switches, Super resolution, Cameras, Microscopy, Luminescence, Molecules, Ions, Image quality, Super resolution microscopy

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Proceedings Article | 22 February 2011 Paper

Amplification of ps-pulses from freely triggerable gain-switched laser diodes at 1062 nm and second harmonic generation in periodically poled lithium niobate

Thomas Schönau, Sina Riecke, Kristian Lauritsen, Rainer Erdmann

Proc. SPIE. 7917, Nonlinear Frequency Generation and Conversion: Materials, Devices, and Applications X

KEYWORDS: Fiber amplifiers, Lithium niobate, Second-harmonic generation, Optical amplifiers, Luminescence, Crystals, Semiconductor lasers, Picosecond phenomena, Laser crystals, Harmonic generation

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Proceedings Article | 11 February 2011 Paper

Fast algorithms for the analysis of spectral FLIM data

I. Gregor, B. Krämer, F. Koberling, R. Erdmann, J. Enderlein, M. Wahl, S. Fore

Proc. SPIE. 7903, Multiphoton Microscopy in the Biomedical Sciences XI

KEYWORDS: Microscopes, Statistical analysis, Luminescence, Fluorescence spectroscopy, Single photon, Fluorescence lifetime imaging, Picosecond phenomena, Fluorescence resonance energy transfer, Data conversion, Signal detection

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Proceedings Article | 26 February 2010 Paper

Fluorescence performance standards for confocal microscopy

Steffen Rüttinger, Peter Kapusta, Volker Völlkopf, Felix Koberling, Rainer Erdmann, Rainer Macdonald

Proc. SPIE. 7569, Multiphoton Microscopy in the Biomedical Sciences X

KEYWORDS: Confocal microscopy, Microscopes, Glasses, Luminescence, Molecules, Diffusion, Fluorescence spectroscopy, Fluorescence correlation spectroscopy, Spatial resolution, Standards development

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Proceedings Article | 26 February 2010 Paper

Fast rasterscanning enables FLIM in macroscopic samples up to several centimeters

F. Koberling, V. Buschmann, C. Hille, M. Patting, C. Dosche, A. Sandberg, A. Wheelock, R. Erdmann

Proc. SPIE. 7569, Multiphoton Microscopy in the Biomedical Sciences X

KEYWORDS: Microscopes, Proteins, Imaging systems, Sensors, Scanners, Luminescence, Single photon, Multiplexing, Laser scanners, Fluorescence lifetime imaging

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Proceedings Article | 25 February 2010 Paper

The use of time-resolved fluorescence in gel-based proteomics for improved biomarker discovery

AnnSofi Sandberg, Volker Buschmann, Peter Kapusta, Rainer Erdmann, Åsa Wheelock

Proc. SPIE. 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII

KEYWORDS: Photon counting, Proteins, Medicine, Visualization, Tissues, Luminescence, Fluorescence spectroscopy, Image acquisition, Laser scanners, Time resolved spectroscopy

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Proceedings Article | 25 February 2009 Paper

New multichannel photon timing instrumentation with independent synchronized channels and high count rate for FLIM and correlation analysis

Michael Wahl, Gerald Kell, Peter Kapusta, Hans-Jürgen Rahn, Tino Roehlicke, Rainer Erdmann

Proc. SPIE. 7183, Multiphoton Microscopy in the Biomedical Sciences IX

KEYWORDS: Photodetectors, Electronics, Sensors, Luminescence, Microchannel plates, Molecules, Fluorescence lifetime imaging, Picosecond phenomena, Fluorescence correlation spectroscopy, Signal detection

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Proceedings Article | 25 February 2009 Paper

Simultaneous monitoring of the two coupled motors of a single FoF1-ATP synthase by three-color FRET using duty cycle-optimized triple-ALEX

N. Zarrabi, S. Ernst, M. Düser, A. Golovina-Leiker, W. Becker, R. Erdmann, S. Dunn, M. Börsch

Proc. SPIE. 7185, Single Molecule Spectroscopy and Imaging II

KEYWORDS: Confocal microscopy, Optical filters, Avalanche photodetectors, Proteins, Luminescence, Acquisition tracking and pointing, Picosecond phenomena, Fluorescence resonance energy transfer, Bragg cells, Pulsed laser operation

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Proceedings Article | 25 February 2009 Paper

Recent advances in photon coincidence measurements for photon antibunching and full correlation analysis

F. Koberling, B. Kraemer, V. Buschmann, S. Ruettinger, P. Kapusta, M. Patting, M. Wahl, R. Erdmann

Proc. SPIE. 7185, Single Molecule Spectroscopy and Imaging II

KEYWORDS: Confocal microscopy, Electronics, Sensors, Luminescence, Molecules, Diffusion, Time metrology, Picosecond phenomena, Fluorescence correlation spectroscopy, Signal detection

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Proceedings Article | 13 February 2009 Paper

Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor FoF1-ATP synthase

M. Börsch, R. Reuter, G. Balasubramanian, R. Erdmann, F. Jelezko, J. Wrachtrup

Proc. SPIE. 7183, Multiphoton Microscopy in the Biomedical Sciences IX

KEYWORDS: Diamond, Proteins, Nanoparticles, Chemical species, Luminescence, Nitrogen, Acquisition tracking and pointing, Fluorescence resonance energy transfer, Nanocrystals, Color centers

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Proceedings Article | 7 February 2009 Paper

Comparison of a Yb-doped fiber and a semiconductor taper for amplification of picosecond laser pulses

Sina Riecke, Kristian Lauritsen, Hendrick Thiem, Katrin Paschke, Rainer Erdmann

Proc. SPIE. 7212, Optical Components and Materials VI

KEYWORDS: Semiconductors, Fiber amplifiers, Indium gallium arsenide, Optical amplifiers, Semiconductor optical amplifiers, Oscillators, Amplifiers, Semiconductor lasers, Picosecond phenomena, Optical isolators

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Proceedings Article | 21 February 2008 Paper

Recent advances in time-correlated single-photon counting

Felix Koberling, Benedikt Krämer, Sebastian Tannert, Steffen Rüttinger, Uwe Ortmann, Matthias Patting, Michael Wahl, Benjamin Ewers, Peter Kapusta, Rainer Erdmann

Proc. SPIE. 6862, Single Molecule Spectroscopy and Imaging

KEYWORDS: Confocal microscopy, Sensors, Luminescence, Photons, Molecules, Diffusion, Light scattering, Picosecond phenomena, Fluorescence correlation spectroscopy, Pulsed laser operation

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Proceedings Article | 21 February 2008 Paper

Latest applications for 2-focus fluorescence correlation spectroscopy

T. Dertinger, I. von der Hocht, A. Loman, R. Erdmann, J. Enderlein

Proc. SPIE. 6862, Single Molecule Spectroscopy and Imaging

KEYWORDS: Refractive index, Prisms, Proteins, Glasses, Luminescence, Molecules, Diffusion, Calcium, Fluorescence correlation spectroscopy, Pulsed laser operation

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Proceedings Article | 20 February 2008 Paper

Fiber amplified and frequency doubled diode lasers as a highly flexible pulse source at 532nm

Kristian Lauritsen, Sina Riecke, Martin Langkopf, Dietmar Klemme, Christopher Kaleva, Christopher Pallassis, Shirley McNeil, Rainer Erdmann

Proc. SPIE. 6871, Solid State Lasers XVII: Technology and Devices

KEYWORDS: Fiber amplifiers, Second-harmonic generation, Phase modulation, Modulation, Crystals, Laser applications, Solid state lasers, Semiconductor lasers, Picosecond phenomena, Laser crystals

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Proceedings Article | 15 February 2008 Paper

Advanced FRET and FCS measurements with laser scanning microscopes based on time-resolved techniques

B. Krämer, V. Buschmann, U. Ortmann, F. Koberling, M. Wahl, M. Patting, P. Kapusta, A. Bülter, R. Erdmann

Proc. SPIE. 6860, Multiphoton Microscopy in the Biomedical Sciences VIII

KEYWORDS: Microscopes, Sensors, Luminescence, Single photon, Data acquisition, Laser scanners, Fluorescence lifetime imaging, Fluorescence correlation spectroscopy, Fluorescence resonance energy transfer, Pulsed laser operation

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Proceedings Article | 15 February 2008 Paper

FLIM and FCS measurements performed with a master oscillator fiber amplifier (MOFA) laser at 530 nm

Felix Koberling, Martin Langkopf, Dietmar Klemme, Andreas Bülter, Volker Buschmann, Kristian Lauritsen, Rainer Erdmann

Proc. SPIE. 6860, Multiphoton Microscopy in the Biomedical Sciences VIII

KEYWORDS: Confocal microscopy, Fiber amplifiers, Microscopes, Luminescence, Fiber lasers, Semiconductor lasers, Fluorescence lifetime imaging, Picosecond phenomena, Fluorescence correlation spectroscopy, Pulsed laser operation

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Proceedings Article | 11 May 2007 Paper

Time-resolved confocal fluorescence microscopy: novel technical features and applications for FLIM, FRET and FCS using a sophisticated data acquisition concept in TCSPC

Felix Koberling, Benedikt Krämer, Peter Kapusta, Matthias Patting, Michael Wahl, Rainer Erdmann

Proc. SPIE. 6583, Photon Counting Applications, Quantum Optics, and Quantum Cryptography

KEYWORDS: Confocal microscopy, Microscopy, Luminescence, Molecules, Data acquisition, Fluorescence lifetime imaging, Picosecond phenomena, Fluorescence correlation spectroscopy, Fluorescence resonance energy transfer, Molecular photonics

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In recent years time-resolved fluorescence measurement and analysis techniques became a standard in single molecule microscopy. However, considering the equipment and experimental implementation they are typically still an add-on and offer only limited possibilities to study the mutual dependencies with common intensity and spectral information. In contrast, we are using a specially designed instrument with an unrestricted photon data acquisition approach which allows to store spatial, temporal, spectral and intensity information in a generalized format preserving the full experimental information. This format allows us not only to easily study dependencies between various fluorescence parameters but also to use, for example, the photon arrival time for sorting and weighting the detected photons to improve the significance in common FCS and FRET analysis schemes. The power of this approach will be demonstrated for different techniques: In FCS experiments the concentration determination accuracy can be easily improved by a simple time-gated photon analysis to suppress the fast decaying background signal. A more detailed analysis of the arrival times allows even to separate FCS curves for species which differ in their fluorescence lifetime but, for example, cannot be distinguished spectrally. In multichromophoric systems like a photonic wire which undergoes unidirectional multistep FRET the lifetime information complements significantly the intensity based analysis and helps to assign the respective FRET partners. Moreover, together with pulsed excitation the time-correlated analysis enables directly to take advantage of alternating multi-colour laser excitation. This pulsed interleaved excitation (PIE) can be used to identify and rule out inactive FRET molecules which cause interfering artefacts in standard FRET efficiency analysis. We used a piezo scanner based confocal microscope with compact picosecond diode lasers as excitation sources. The timing performance can be significantly increased by using new SPAD detectors which enable, in conjunction with new TCSPC electronics, an overall IRF width of less than 120 ps maintaining single molecule sensitivity.

Proceedings Article | 11 May 2007 Paper

Dead-time effects in TCSPC data analysis

Matthias Patting, Michael Wahl, Peter Kapusta, Rainer Erdmann

Proc. SPIE. 6583, Photon Counting Applications, Quantum Optics, and Quantum Cryptography

KEYWORDS: Photodetectors, Data modeling, Sensors, Luminescence, Error analysis, Single photon, Time correlated photon counting, Picosecond phenomena, Monochromators, Data analysis

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Proceedings Article | 11 May 2007 Paper

Advanced TCSPC electronics and their use in general quantum physics and demanding analytical applications

Michael Wahl, Hans-Juergen Rahn, Matthias Patting, Felix Koberling, Rainer Erdmann

Proc. SPIE. 6583, Photon Counting Applications, Quantum Optics, and Quantum Cryptography

KEYWORDS: Electronics, Sensors, Luminescence, Photons, Molecules, Time metrology, Quantum physics, Fluorescence lifetime imaging, Picosecond phenomena, Fluorescence correlation spectroscopy

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Proceedings Article | 27 February 2006 Paper

Measuring precise diffusion coefficients with two-focus fluorescence correlation spectroscopy

Thomas Dertinger, Ingo Gregor, Iris von der Hocht, Rainer Erdmann, Benedikt Krämer, Felix Koberling, Rudolf Hartmann, Jörg Enderlein

Proc. SPIE. 6092, Ultrasensitive and Single-Molecule Detection Technologies

KEYWORDS: Photodetectors, Beam splitters, Data modeling, Polarization, Luminescence, Molecules, Diffusion, Objectives, Fluorescence correlation spectroscopy, Pulsed laser operation

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Proceedings Article | 27 February 2006 Paper

Identification of triplet state and photoisomerization of Cy5 by means of ensemble and single-molecule measurements

Zhengxi Huang, Dongmei Ji, Sufan Wang, Andong Xia, Felix Koberling, Matthias Patting, Rainer Erdmann

Proc. SPIE. 6092, Ultrasensitive and Single-Molecule Detection Technologies

KEYWORDS: Chemical species, Glasses, Luminescence, Molecules, Bioalcohols, Phosphorescence, Molecular lasers, Spectrophotometry, Photolysis, Absorption

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Proceedings Article | 28 April 2005 Paper

Amplified picosecond diode lasers for diffuse optical imaging and spectroscopy of tissue

R. Erdmann, M. Langkopf, K. Lauritsen, A. Bulter, M. Wahl, H. Wabnitz, A. Liebert, M. Moller, T. Schmitt

Proc. SPIE. 5693, Optical Tomography and Spectroscopy of Tissue VI

KEYWORDS: Spectroscopy, Imaging spectroscopy, Semiconductor lasers, Optical spectroscopy, Picosecond phenomena, Optical tomography, Tissue optics, Laser tissue interaction, Optical spectroscopy imaging, Diffuse optical imaging

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Proceedings Article | 27 April 2005 Paper

10-W peak power from a gain-switched picosecond all-semiconductor laser

Reto Haring, Thorsten Schmitt, Aude-Reine Bellancourt, Frank Lison, Kristian Lauritsen, Rainer Erdmann, Wilhelm Kaenders

Proc. SPIE. 5707, Solid State Lasers XIV: Technology and Devices

KEYWORDS: Second-harmonic generation, Optical amplifiers, Oscillators, Microscopy, Semiconductor lasers, Solids, Diodes, Picosecond phenomena, Pulsed laser operation, Laser systems engineering

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Proceedings Article | 30 March 2005 Paper

Time-resolved laser scanning microscopy with FLIM and advanced FCS capability

Benedict Kraemer, Felix Koberling, Uwe Ortmann, Michael Wahl, Peter Kapusta, Andreas Buelter, Rainer Erdmann

Proc. SPIE. 5700, Multiphoton Microscopy in the Biomedical Sciences V

KEYWORDS: Microscopes, Sensors, Microscopy, Luminescence, Semiconductor lasers, Data acquisition, Laser scanners, Fluorescence lifetime imaging, Picosecond phenomena, Fluorescence correlation spectroscopy

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We present the technical integration of state-of-the-art picosecond diode laser sources and data acquisition electronics in conventional laser scanning microscopes. This procedure offers users of laser scanning microscopes an easy upgrade path towards time-resolved measurements. Our setup uses picosecond diode lasers from 375 to 800 nm for excitation which are coupled to the microscope via a single mode fiber. The corresponding emission is guided to a fibre coupled photon counting detector, such as Photomultiplier Tubes (PMT) or Single Photon Avalanche Diodes (SPAD). This combines the outstanding sensitivity of photon counting detectors with the ease of use of diode laser sources, to allow time-resolved measurements of fluorescence decays with resolutions down to picoseconds. The synchronization signals from the laser scanning microscope are fed into the data stream recorded by the TimeHarp 200 TCSPC system, via the unique Time-Tagged Time-Resolved (TTTR) data acquisition mode. In this TTTR data acquisition mode each photon is recorded individually with its specific parameters as detector channel, picosecond timing, global arrival time and, in this special application, up to three additional markers. These markers, in combination with the global arrival time, allow the system software to reconstruct the complete image and subsequently create the full fluorescence lifetime image (FLIM). The multi-parameter data acquisition scheme of the TimeHarp 200 electronics not only records each parameter individually, but offers in addition the opportunity to analyse the parameter dependencies in a multitude of different ways. This method allows for example to calculate the fluorescence fluctuation correlation function (FCS) on any single spot of interest but also to reconstruct the fluorescence decay of each image pixel and detector channel for advanced Forster Resonance Energy Transfer (FRET) analysis. In this paper, we present some selected results acquired with standard laser scanning microscopes upgraded towards temporal resolution.

Proceedings Article | 29 March 2005 Paper

Advanced multifocus confocal laser scanning microscope for single molecule studies

Thomas Dertinger, Felix Koberling, Ales Benda, Rainer Erdmann, Martin Hof, Joerg Enderlein

Proc. SPIE. 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III

KEYWORDS: Confocal microscopy, Microscopes, Photodetectors, Particles, Luminescence, Molecules, Diffusion, Fluorescence spectroscopy, Fluorescence correlation spectroscopy, Pulsed laser operation

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Proceedings Article | 18 June 2004 Paper

Compact TCSPC upgrade package for laser scanning microscopes based on 375- to 470-nm picosecond diode lasers

Uwe Ortmann, T. Dertinger, Michael Wahl, Matthias Patting, Rainer Erdmann

Proc. SPIE. 5325, Optical Diagnostics and Sensing IV

KEYWORDS: Microscopes, Photon counting, Sensors, Luminescence, Semiconductor lasers, Data acquisition, Laser scanners, Fluorescence lifetime imaging, Picosecond phenomena, Signal detection

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We present the technical integration of state-of-the-art picosecond diode laser sources and data acquisition electronics in conventional laser scanning microscopes. This offers users of laser scanning microscopes an easy upgrade path towards time-resolved measurements. Our setup uses picosecond diode lasers from 375 nm, 405 nm, 440 nm and 470 nm for fluorescence excitation which are coupled in through a sole single mode fiber. The detected signal is guided to a photon counting detector, such as Photomultiplier Tubes (PMT) or Single Photon Avalanche Diodes (SPAD). This combines the outstanding sensitivity of photon counting detectors with the ease of use of diode laser sources, to allow time-resolved measurements of fluorescence decays with resolutions down to picoseconds. The synchronization signals from the laser scanning microscope are fed into the data stream recorded by the TimeHarp 200 TCSPC5,7 system, via the unique Time-Tagged Time-Resolved (TTTR)6 data acquisition mode. In this TTTR data acquisition mode each photon is recorded individually with its specific parameters as detector channel, picosecond timing, global arrival time and, in this special application, up to three additional markers. These markers, in combination with the global arrival time, allow the system software to reconstruct the complete image and subsequently fit the full fluorescence lifetime image. The multi-parameter data acquisition scheme of the TimeHarp 200 electronics not only records each parameter individually, but offers in addition the opportunity to analyse the parameter dependencies in a multitude of different ways. This method allows not only to calculate the fluorescence fluctuation correlation function (FCS) on any single spot of interest but also to reconstruct the fluorescence decay of each image pixel and detector channel for the purpose of Fluorescence Lifetime Imaging (FLIM) or advanced Fluorescence Resonance Energy Transfer (FRET) analysis. We present here some selected results acquired with standard laser scanning microscopes upgraded for the time-correlated single photon counting technique.

Proceedings Article | 14 October 2003 Paper

Two-channel fluorescence lifetime microscope with two colour laser excitation, single-molecule sensitivity, and submicrometer resolution

Felix Koberling, Michael Wahl, Matthias Patting, Hans-Juergen Rahn, Peter Kapusta, Rainer Erdmann

Proc. SPIE. 5143, Novel Optical Instrumentation for Biomedical Applications

KEYWORDS: Microscopes, Electronics, Polarization, Sensors, Luminescence, Molecules, Single photon, Fluorescence lifetime imaging, Picosecond phenomena, Fluorescence correlation spectroscopy

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SPIE Journal Paper | 1 January 2003

Time-resolved microspectrofluorometry and fluorescence lifetime imaging of photosensitizers using picosecond pulsed diode lasers in laser scanning microscopes

Matthias Kress, Thomas Meier, Rudolf Steiner, Frank Dolp, Rainer Erdmann, Uwe Ortmann, Angelika Rueck

JBO Vol. 8 Issue 01

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This work describes the time-resolved fluorescence characteristics of two different photosensitizers in single cells, in detail mTHPC and 5-ALA induced PPIX, which are currently clinically used in photodynamic therapy. The fluorescence lifetime of the drugs was determined in the cells from time-gated spectra as well as single photon counting, using a picosecond pulsed diode laser for fluorescence excitation. The diode laser, which emits pulses at 398 nm with 70 ps full width at half maximum duration, was coupled to a confocal laser scanning microscope. For time-resolved spectroscopy a setup consisting of a Czerny Turner spectrometer and a MCP-gated and -intensified CCD camera was used. Time-gated spectra within the cells were acquired by placing the laser beam in ‘‘spot scan’’ mode. In addition, a time-correlated single photon counting module was used to determine the fluorescence lifetime from single spots and to record lifetime images. The fluorescence lifetime of mTHPC decreased from 7.5 to 5.5 ns during incubation from 1 to 6 h. This decrease was probably attributed to enhanced formation of aggregates during incubation. Fluorescence lifetime imaging showed that longer lifetimes were correlated with accumulation in the cytoplasm in the neighborhood of the cell nucleus, whereas shorter lifetimes were found in the outer cytoplasm. For cells that were incubated with 5-ALA, a fluorescence lifetime of 7.4 ns was found for PPIX; a shorter lifetime at 3.6 ns was probably attributed to photoproducts and aggregates of PPIX. In contrast from fluorescence intensity images alone, different fluorescence species could not be distinguished. However, in the lifetime image a structured fluorescence distribution in the cytoplasm was correlated with the longer lifetime and probably coincides with mitochondria. In conclusion, picosecond diode lasers coupled to a laser scanning microscope equipped with appropriate detection units allows time-resolved spectroscopy and lifetime imaging with high spatial resolution and provides numerous possibilities in cellular and pharmaceutical research.

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Proceedings Article | 16 April 2002 Paper

Application of sub-ns pulsed LEDs in fluorescence lifetime spectroscopy

Michael Wahl, Uwe Ortmann, Kristian Lauritsen, Rainer Erdmann

Proc. SPIE. 4648, Test and Measurement Applications of Optoelectronic Devices

KEYWORDS: Light sources, Electronics, Light emitting diodes, Spectroscopy, Luminescence, Molecules, Fluorescence spectroscopy, Single photon, Semiconductor lasers, Picosecond phenomena

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Proceedings Article | 28 March 2002 Paper

Fluorescence lifetime imaging system with nm-resolution and single-molecule sensitivity

Michael Wahl, Hans-Juergen Rahn, Uwe Ortmann, Rainer Erdmann, Martin Boehmer, Joerg Enderlein

Proc. SPIE. 4634, Methods for Ultrasensitive Detection II

KEYWORDS: Electronics, Clocks, Sensors, Scanners, Luminescence, Molecules, Single photon, Semiconductor lasers, Fluorescence lifetime imaging, Picosecond phenomena

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Proceedings Article | 3 May 1999 Paper

Simple scanning near-infrared time-correlated single-photon counting instrument with a pulsed diode laser and avalanche photodiode (APD) for time-resolved measurements in scanning applications with

Yuling Zhang, Steven Soper, Lyle Middendorf, John Wrum, Rainer Erdmann, Michael Wahl

Proc. SPIE. 3602, Advances in Fluorescence Sensing Technology IV

KEYWORDS: Microscopes, Avalanche photodetectors, Sensors, Luminescence, Single photon, Semiconductor lasers, Head, Aluminum, Picosecond phenomena, Avalanche photodiodes

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Proceedings Article | 10 April 1998 Paper

Hardware solution for continuous time-resolved burst detection of single molecules in flow

Michael Wahl, Rainer Erdmann, Kristian Lauritsen, Hans-Juergen Rahn

Proc. SPIE. 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery

KEYWORDS: Photon counting, Luminescence, Molecules, Single photon, Field programmable gate arrays, Semiconductor lasers, Time metrology, Optical resolution, Picosecond phenomena, Liquids

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Proceedings Article | 22 May 1997 Paper

All-solid-state TCPC instrument for dynamic lifetime measurements in sensitive DNA analysis

Rainer Erdmann, Michael Wahl, Kristian Lauritsen, Joerg Enderlein, Benjamin Legendre, Daryl Williams, Steven Soper

Proc. SPIE. 2985, Ultrasensitive Biochemical Diagnostics II

KEYWORDS: Electronics, Capillaries, Luminescence, Single photon, Light scattering, Solid state lasers, Semiconductor lasers, Picosecond phenomena, Solid state electronics, Electro optical modeling

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Proceedings Article | 1 April 1996 Paper

High-repetion-rate picosecond diode lasers for potential use in single-molecule detection

Uwe Ortmann, Rolf Krahl, Gerald Kell, Rainer Erdmann, Joerg Enderlein, Edgar Klose

Proc. SPIE. 2680, Ultrasensitive Biochemical Diagnostics

KEYWORDS: Electronics, Lasers, Molecules, Fluorescence spectroscopy, Single photon, Semiconductor lasers, Picosecond phenomena, Molecular lasers, Laser systems engineering, Liquids

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Proceedings Article | 1 April 1996 Paper

High-speed electronics for the detection of time-resolved fluorescence in a continuous flow system

Rainer Erdmann, Uwe Ortmann, Joerg Enderlein, Wolfgang Becker, Michael Wahl, Edgar Klose

Proc. SPIE. 2680, Ultrasensitive Biochemical Diagnostics

KEYWORDS: Clocks, Luminescence, Photons, Molecules, Fluorescence spectroscopy, Laser induced fluorescence, Picosecond phenomena, Time resolved spectroscopy, Molecular lasers, High speed electronics

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Proceedings Article | 8 May 1995 Paper

Simultaneous detection of time-resolved emission spectra using a multianode PMT and new time-correlated single-photon counting (TCSPC) electronics with a 5-MHz count rate

Rainer Erdmann, Wolfgang Becker, Uwe Ortmann, Joerg Enderlein

Proc. SPIE. 2388, Advances in Fluorescence Sensing Technology II

KEYWORDS: Ultrafast phenomena, Electronics, Sensors, Luminescence, Single photon, Time metrology, Picosecond phenomena, Oscilloscopes, Monochromators, Signal detection

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Proceedings Article | 8 May 1995 Paper

Lifetime-based identification of single molecules

Joerg Enderlein, Rolf Krahl, Uwe Ortmann, Michael Wahl, Rainer Erdmann, Edgar Klose

Proc. SPIE. 2388, Advances in Fluorescence Sensing Technology II

KEYWORDS: Electronics, Luminescence, Photons, Molecules, Bioalcohols, Time metrology, Data processing, Molecular lasers, Signal detection, Rhodamine

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Proceedings Article | 8 May 1995 Paper

Electron transfer dyes as standard for picosecond fluorescence decay measurements

Jens Sobek, Arno Wiehe, Joerg Enderlein, Rainer Erdmann, Harry Kurreck

Proc. SPIE. 2388, Advances in Fluorescence Sensing Technology II

KEYWORDS: Luminescence, Quantum efficiency, Zinc, Electroluminescence, Picosecond phenomena, Antimony, Systems modeling, Catalysis, Absorption, Oxidation

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Proceedings Article | 22 December 1994 Paper

Very compact all solid state fluorescence lifetime measurement system: preliminary results

Rainer Erdmann, Gerald Kell, Rolf Krahl, Uwe Ortmann, Wolfgang Becker, Joerg Enderlein, Edgar Klose

Proc. SPIE. 2328, Biomedical Optoelectronic Devices and Systems II

KEYWORDS: Ultrafast phenomena, Electronics, Sensors, Luminescence, Single photon, Semiconductor lasers, Diodes, Picosecond phenomena, Monochromators, Signal detection

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Proceedings Article | 21 July 1994 Paper

New compact TCSPC apparatus based on sub 15 ps laserdiodes: preliminary results

Rainer Erdmann, Gerald Kell, Wolfgang Becker, Edgar Klose

Proc. SPIE. 2136, Biochemical Diagnostic Instrumentation

KEYWORDS: Ultrafast phenomena, Luminescence, Single photon, Laser development, Data acquisition, Diodes, Sapphire lasers, Picosecond phenomena, Stray light, Pulsed laser operation

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Proceedings Article | 24 June 1993 Paper

Investigations in high-speed DNA sequencing

Rainer Erdmann, Claus Seidel, F. Schubert, Edgar Klose

Proc. SPIE. 1895, Ultrasensitive Laboratory Diagnostics

KEYWORDS: Second-harmonic generation, Luminescence, Molecules, Fluorescence spectroscopy, Single photon, Diagnostics, Picosecond phenomena, Signal detection, Absorption, Data analysis

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Showing 5 of 62 publications

Proceedings Volume Editor (13)

SPIE Conference Volume | 9 April 2021

Single Molecule Spectroscopy and Superresolution Imaging XIV

SPIE Conference Volume | 3 March 2020

Single Molecule Spectroscopy and Superresolution Imaging XIII

SPIE Conference Volume | 21 May 2018

Single Molecule Spectroscopy and Superresolution Imaging XI

SPIE Conference Volume | 28 April 2017

Single Molecule Spectroscopy and Superresolution Imaging X

SPIE Conference Volume | 7 July 2016

Single Molecule Spectroscopy and Superresolution Imaging IX

Showing 5 of 13 publications

Conference Committee Involvement (15)

Single Molecule Spectroscopy and Superresolution Imaging XV

22 January 2022 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XIV

6 March 2021 | Online Only, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XIII

1 February 2020 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XII

2 February 2019 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XI

27 January 2018 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging X

28 January 2017 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging IX

13 February 2016 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VIII

7 February 2015 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VII

1 February 2014 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VI

2 February 2013 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging V

21 January 2012 | San Francisco, California, United States

Single Molecule Spectroscopy and Imaging IV

22 January 2011 | San Francisco, California, United States

Single Molecule Spectroscopy and Imaging III

23 January 2010 | San Francisco, California, United States

Single Molecule Spectroscopy and Imaging II

24 January 2009 | San Jose, California, United States

Single Molecule Spectroscopy and Imaging

19 January 2008 | San Jose, California, United States

Showing 5 of 15 Conference Committees

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