Ms. Renee M. Korol Profile

Renee M. Korol

PhD Candidate at Western Univ Canada

SPIE Involvement:

Author

Publications (3)

SPIE Journal Paper | 1 March 2007 Open Access

Fluorescence spectroscopy and birefringence of molecular changes in maturing rat tail tendon

Renee Korol, Helen Finlay, Melanie Josseau, Alexandra Lucas, Peter Canham

JBO, Vol. 12, Issue 02, 024011, (March 2007) https://doi.org/10.1117/12.10.1117/1.2714055

KEYWORDS: Collagen, Birefringence, Skin, Tissues, Luminescence, Proteins, Fluorescence spectroscopy, Tissue optics, Laser induced fluorescence, Molecular spectroscopy

Read Abstract +

DOWNLOAD PAPER

Proceedings Article | 8 October 2005 Paper

Optical detection of structural changes in human carotid atherosclerotic plaque

R. Korol, P. Canham, H. Finlay, R. Hammond, M. Quantz, G. Ferguson, L. Liu, A. Lucas

Proceedings Volume 5862, 58620J (2005) https://doi.org/10.1117/12.633023

KEYWORDS: Collagen, Arteries, Tissues, Laser induced fluorescence, Luminescence, Proteins, Natural surfaces, Fluorescence spectroscopy, Spectroscopy, Surgery

Read Abstract +

Background: Arterial bifurcations are commonly the sites of developing atherosclerotic plaque that lead to arterial occlusions and plaque rupture (myocardial infarctions and strokes). Laser induced fluorescence (LIF) spectroscopy provides an effective nondestructive method supplying spectral information on extracellular matrix (ECM) protein composition, specifically collagen and elastin. Purpose: To investigate regional differences in the ECM proteins -- collagen I, III and elastin in unstable plaque by analyzing data from laser-induced fluorescence spectroscopy of human carotid endarterectomy specimens. Methods: Gels of ECM protein extracts (elastin, collagen types I & III) were measured as reference spectra and internal thoracic artery segments (extra tissue from bypass surgery) were used as tissue controls. Arterial segments and the endarterectomy specimens (n=21) were cut into 5mm cross-sectional rings. Ten fluorescence spectra per sampling area were then recorded at 5 sites per ring with argon laser excitation (357nm) with a penetration depth of 200 μm. Spectra were normalized to maximum intensity and analyzed using multiple regression analysis. Tissue rings were fixed in formalin (within 3 hours of surgery), sectioned and stained with H&E or Movat's Pentachrome for histological analysis. Spectroscopy data were correlated with immunohistology (staining for elastin, collagen types I, III and IV). Results: Quantitative fluorescence for the thoracic arteries revealed a dominant elastin component on the luminal side -- confirmed with immunohistology and known artery structure. Carotid endarterectomy specimens by comparison had a significant decrease in elastin signature and increased collagen type I and III. Arterial spectra were markedly different between the thoracic and carotid specimens. There was also a significant elevation (p<0.05) of collagen type I distal to the bifurcation compared to proximal tissue in the carotid specimens. Conclusion: Fluorescence spectroscopy is an effective method for evaluating ECM (collagen and elastin) associated with vascular remodeling despite the considerable variability in the plaque structure. Consistent regional differences were detected in the carotid specimens.

Proceedings Article | 2 October 2003 Paper

Semiquantitative analysis of atherosclerotic plaque using optical coherence tomography and time-of-flight secondary ion mass spectrometry

Renee Korol, Babajide Togonu-Bickersteth, Victor Yang, Stamen Dimov, Pracha Vatsya, Maggie Gordon, Alex Vitkin, Liying Liu, Peter Canham, Sharon Clarke, Alexandra Lucas

Proceedings Volume 5140, (2003) https://doi.org/10.1117/12.500973

KEYWORDS: Optical coherence tomography, Calcium, Ions, Tissues, Potassium, Mass spectrometry, Tissue optics, Natural surfaces, Sodium, Chemical analysis

Read Abstract +

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years