Mr. Sergei K. Belenok Profile

Sergei K. Belenok

at National Research Nuclear Univ MEPhI

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Publications (2)

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Proceedings Article | 12 September 2021 Presentation + Paper

Portable aerosol sampler with liquid phase for collection of biopathogens at subzero temperatures

Artem Akmalov, Sergei Belenok, Vitaly Kostarev, Gennadii Kotkovskii, Alexander Chistyakov

Proceedings Volume 11869, 118690B (2021) https://doi.org/10.1117/12.2597974

KEYWORDS: Liquids, Particles, Aerosols, Biological research, Atmospheric particles, Bioalcohols, Microorganisms

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Aerosol samplers with a recirculating liquid film are promising devices for remote biological monitoring. The presence of the liquid film provides a high survival rate for biological objects. The relatively simpler design allows portability to the sampler, which will make it possible to conduct tests outside the laboratory. In this study an analytical expression, describing the capturing efficiency of aerosol particles in the water film, taking into account the friction forces arising from interaction of water and air in a cyclone-based aerosol collector, was obtained. A new element, took over from the theory of centrifugal sprayers - a vortex chamber, was added to the theory and design of the collector. It allows increasing the initial angular moment of the elements of air volume entering the collector, which leads in appropriately an increase of maximal height of rising liquid film and particle capturing efficiency. To analyze the obtained expressions, graphs of particle capturing efficiency on basic parameters of modified cyclone collector and volumetric air flow were calculated. The graphs made it possible to determine the optimal geometric parameters for the portable cyclone-based collector. The introduced dependence on viscosity made it possible to estimate more accurately the efficiency of the device at various temperatures (including negative temperatures). For the selected parameters, graphs particle capturing efficiency were plotted. Water-alcohol solution and Novec 1230 fluid were used as fluids capable of operating at subzero temperatures. To check the operability of the sampler, tests were carried out to collect samples of sprayed inactivated adenovirus in a microbiological safety box at the Gamaleya Institute. The results of tests are discussed.

Proceedings Article | 20 September 2020 Presentation + Paper

Portable aerosol collector with liquid circulation mounted on a drone

Artem Akmalov, Sergei Belenok, Anastasia Kadyrova, Veronika Kirillova, Vitaly Kostarev, Gennadii Kotkovskii, Alexander Chistyakov

Proceedings Volume 11542, 115420J (2020) https://doi.org/10.1117/12.2572653

KEYWORDS: Aerosols, Liquids, Statistical analysis, Atmospheric particles, Microorganisms, Biological research, Pathogens, Solar concentrators, Atmospheric monitoring, Particles

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Early detection of pathogens is crucial for ensuring safe living conditions. Conventional sampling methods do not ensure the sustainability of captured microorganisms. Cyclone-based liquid absorptive samplers are the most promising devices in this regard. Most samplers have limited application scope as they are intended for laboratories. In the study we developed a special liquid-based sampler for mounting on a drone. A structure and base values of a cyclone collector were determined. The chosen design provides maximum particle capturing efficiency in terms of low air flow rate and small size of the collector. Projected capturing efficiency for particles of the size of 1mсm is approximately 20%, for particles of the size bigger than 2,5mсm is 100%. Mathematical modeling proved the correctness of theoretical considerations. This made it possible to design and fabricate a prototype of a sampler device which also includes a fan, a peristaltic pump, valves, a sensor for liquid levels monitoring, a display for operation mode controlling. Outdoor sampling on a drone has successfully proved performance efficiency at close to zero temperatures. A study on capturing efficiency was carried out by sampling sucrose aerosol with a fluorescein dye in a microbiological safety box. Specific efficiency of our sampler is 1,18 [relative units/(litre/min)], which is over twice s.e. of 0,43 [r.u./(l/min)] of existing laboratory devices. The device provides for the issuance of collected samples on standard immunochemical test strips for the analysis of adenovirus, rotavirus, influenza and other respiratory diseases with a full analysis time of 10 minutes.

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