Dr. Sergey Turtaev Profile

Dr. Sergey Turtaev

PHD Student at Leibniz-IPHT

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Author

Publications (8)

SPIE Journal Paper | 13 February 2024 Open Access

Advancing the path to in-vivo imaging in freely moving mice via multimode-multicore fiber based holographic endoscopy

Yang Du, Evelyn Dylda, Miroslav Stibůrek, André D Gomes, Sergey Turtaev, Janelle M. P. Pakan, Tomáš Čižmár

NPh, Vol. 11, Issue S1, S11506, (February 2024) https://doi.org/10.1117/12.10.1117/1.NPh.11.S1.S11506

KEYWORDS: In vivo imaging, Multicore fiber, Multimode fibers, Brain, Animals, Structured optical fibers, Neurons, Digital micromirror devices, Neurophotonics, Neuroimaging

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SignificanceHair-thin multimode optical fiber-based holographic endoscopes have gained considerable interest in modern neuroscience for their ability to achieve cellular and even subcellular resolution during in-vivo deep brain imaging. However, the application of multimode fibers in freely moving animals presents a persistent challenge as it is difficult to maintain optimal imaging performance while the fiber undergoes deformations.AimWe propose a fiber solution for challenging in-vivo applications with the capability of deep brain high spatial resolution imaging and neuronal activity monitoring in anesthetized as well as awake behaving mice.ApproachWe used our previously developed M3CF multimode-multicore fiber to record fluorescently labeled neurons in anesthetized mice. Our M3CF exhibits a cascaded refractive index structure, enabling two distinct regimes of light transport that imitate either a multimode or a multicore fiber. The M3CF has been specifically designed for use in the initial phase of an in-vivo experiment, allowing for the navigation of the endoscope’s distal end toward the targeted brain structure. The multicore regime enables the transfer of light to and from each individual neuron within the field of view. For chronic experiments in awake behaving mice, it is crucial to allow for disconnecting the fiber and the animal between experiments. Therefore, we provide here an effective solution and establish a protocol for reconnection of two segments of M3CF with hexagonally arranged corelets.ResultsWe successfully utilized the M3CF to image neurons in anaesthetized transgenic mice expressing enhanced green fluorescent protein. Additionally, we compared imaging results obtained with the M3CF with larger numerical aperture (NA) fibers in fixed whole-brain tissue.ConclusionsThis study focuses on addressing challenges and providing insights into the use of multimode-multicore fibers as imaging solutions for in-vivo applications. We suggest that the upcoming version of the M3CF increases the overall NA between the two cladding layers to allow for access to high resolution spatial imaging. As the NA increases in the multimode regime, the fiber diameter and ring structure must be reduced to minimize the computational burden and invasiveness.

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SPIE Journal Paper | 19 January 2024 Open Access

“There’s plenty of room at the bottom”: deep brain imaging with holographic endo-microscopy

Hana Uhlířová, Miroslav Stibůrek, Tomáš Pikálek, André Gomes, Sergey Turtaev, Petra Kolbábková, Tomáš Čižmár

NPh, Vol. 11, Issue S1, S11504, (January 2024) https://doi.org/10.1117/12.10.1117/1.NPh.11.S1.S11504

KEYWORDS: Holography, Brain, Digital micromirror devices, In vivo imaging, Animals, Brain imaging, Wavefronts, Biological imaging, Neuroimaging, Image resolution

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Proceedings Article | 3 March 2022 Presentation + Paper

3D imaging through a single optical fiber

Daan Stellinga, David Phillip, Simon Mekhail, Adam Selyem, Sergey Turtaev, Tomáš Cižmár, Miles Padgett

Proceedings Volume 12016, 120160M (2022) https://doi.org/10.1117/12.2616915

KEYWORDS: Optical fibers, Imaging systems, Multimode fibers, Digital micromirror devices

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Proceedings Article | 4 March 2019 Presentation

In-vivo deep-brain imaging through a single fibre endoscope (Conference Presentation)

Sergey Turtaev, Ivo Leite, Tristan Altwegg-Boussac, Janelle M. Pakan, Nathalie Rochefort, Tomas Cizmar

Proceedings Volume 10886, 1088604 (2019) https://doi.org/10.1117/12.2510432

KEYWORDS: Endoscopes, In vivo imaging, Neuroscience, Tissues, Holography, Multimode fibers, Image resolution, Imaging systems, Visual process modeling, Mode conditioning cables

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Proceedings Article | 4 March 2019 Presentation

Holographic optical tweezers at the tip of a multimode fibre (Conference Presentation)

Ivo Leite, Sergey Turtaev, Xin Jiang, Martin Siler, Philip Russell, Tomáš Čižmár

Proceedings Volume 10886, 108860M (2019) https://doi.org/10.1117/12.2510418

KEYWORDS: Holography, Optical tweezers, Multimode fibers, Geometrical optics, Dielectrics, Step index fibers, Sodium, Microscopy

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Proceedings Article | 4 March 2019 Paper

Exploiting digital micromirror device for holographic micro-endoscopy

Sergey Turtaev, Ivo Leite, Kevin Mitchell, Miles Padgett, David Phillips, Tomáš Čižmár

Proceedings Volume 10932, 1093203 (2019) https://doi.org/10.1117/12.2509429

KEYWORDS: Digital micromirror devices, Spatial light modulators

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Proceedings Article | 15 March 2018 Presentation

Predicting the transmission matrix of graded index media (Conference Presentation)

Dirk Boonzajer Flaes, Jan Stopka, Sergey Turtaev, Stefan Witte, Johannes de Boer, Tom Tyc, Tomáš Čižmár

Proceedings Volume 10502, 105021C (2018) https://doi.org/10.1117/12.2290678

KEYWORDS: Refractive index, Graded index fibers, Multimode fibers, Tissue optics, Light scattering, Image resolution, Endoscopy, Geometrical optics, Cladding, Step index fibers

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Proceedings Article | 24 April 2017 Presentation

High-speed wavefront modulation in complex media (Conference Presentation)

Sergey Turtaev, Ivo Leite, Tomáš Cizmár

Proceedings Volume 10073, 1007313 (2017) https://doi.org/10.1117/12.2253151

KEYWORDS: Light wave propagation, Scattering media, Biomedical optics, Optical micromanipulation, Wavefronts, Spatial light modulators, Light scattering, High speed imaging, Modulation, Modulators

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Showing 5 of 8 publications

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