Dr. Seungkyoung Yang Profile

Dr. Seungkyoung Yang

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Proceedings Article | 20 May 2013 Paper

An addressable cell array for a platform of biosensor chips

Seungkyoung Yang, Soo-hee Choi, Moon Youn Jung, Kibong Song, Jeong Won Park

Proceedings Volume 8879, 88790W (2013) https://doi.org/10.1117/12.2018587

KEYWORDS: Signal detection, Biosensors, Sensors, Olfactory system, Receptors, Luminescence, Neurons, Complex systems, Computer aided design, Microscopes

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In order to detect interested matters in fields, various lab-on-a-chips where chemical, physical, or biological sensors are loaded have been developed. eNOSE can be a representative example among them. Because animals can sense 300~1000 different chemicals by olfactory system – smell –, the olfactory system has been spotlighted as new materials in the field of sensing. Those investigations, however, are usually focused on how to detect signals from the olfactory neurons or receptors loaded on chips and enhance sensing efficacy of chips. Therefore, almost of those chips are designed for only one material sensing. Multi-sensing using multi-channels will be needed when the olfactory systems are adopted well on chips. For multiple sensing, we developed an addressable cell array. The chip has 38 cell-chambers arranged in a circle shape and different cell types of thirty eight can be allocated with specific addresses on the chip without any complex valve system. In order to confirm the cell addressing, we loaded EGFP-transfected and empty vector-transfected HEK293a cells into inlets of the cell array in a planned address and those cells were positioned into each chamber by brief aspiration. The arrayed cells were confirmed as a specific pattern through EGFP and nuclei staining. This cell array which can generate address of sensor materials like cells with their own specification is expected to be applied to a platform for a biosensor chip at various sensing fields.

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