Prof. Simon M. Ameer-Beg Profile

Prof. Simon M. Ameer-Beg

Professor of Biophysics at King's College London

SPIE Involvement:

Author

Publications (30)

Proceedings Article | 13 March 2024 Presentation

Detection of protein-protein interactions within biological nano-domains by single molecule programmable array microscopy

Simon Ameer-Beg, Conor Treacy, Simon Poland, Justin Aluko, Thomas Kavanagh, Michael Boersch

Proceedings Volume PC12847, PC128470T (2024) https://doi.org/10.1117/12.3002714

KEYWORDS: Molecular interactions, Molecules, Biological imaging, Nanodomains, Time correlated single photon counting, Molecular spectroscopy, Cancer detection, Single molecule spectroscopy, Quantum detection, Lung cancer

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Proceedings Article | 15 March 2023 Presentation

Microscopy Innovation Centre KCL: A facility for bespoke microscopy (Conference Presentation)

Dylan Herzog, Nicholas Anthony, Susan Cox, Simon Ameer-Beg, Maddy Parsons

Proceedings Volume PC12385, PC123850D (2023) https://doi.org/10.1117/12.2648351

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Proceedings Article | 15 March 2023 Presentation

Mechanosensitive protein-protein interactions in nascent focal adhesions determined by three-colour FRET sensing using multiphoton fluorescence lifetime imaging microscopy (Conference Presentation)

Conor Treacy, Ambrish Kumar, Tam Bui, Simon Poland, Mark Pfuhl, Maddy Parsons, Simon Ameer-Beg

Proceedings Volume PC12384, PC123840F (2023) https://doi.org/10.1117/12.2649187

KEYWORDS: Multiphoton fluorescence microscopy, Multiphoton microscopy, Microscopy, Fluorescence resonance energy transfer

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Proceedings Article | 9 March 2020 Presentation

High speed 2-photon fluorescence lifetime imaging of protein-protein interactions with a swept array microscope (Conference Presentation)

Simon Ameer-Beg, Simon Poland, Thomas Kavanagh, James Levitt, Robert Henderson, Hanning Mai, Andrea Serio, Conor Treacy, Kelly O'Toole, Richard Walker

Proceedings Volume 11244, 112441A (2020) https://doi.org/10.1117/12.2544220

KEYWORDS: Fluorescence lifetime imaging, Microscopes, Laser scanners, Multiphoton microscopy, Microscopy, Live cell imaging, Imaging systems, Cameras, Biosensors, Luminescence

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Proceedings Article | 17 February 2020 Presentation + Paper

Flow cytometry visualization and real-time processing with a CMOS SPAD array and high-speed hardware implementation algorithm

Hanning Mai, Simon Poland, Francesco Mattioli Della Rocca, Conor Treacy, Justin Aluko, Jakub Nedbal, Ahmet Erdogan, Istvan Gyongy, Richard Walker, Simon Ameer-Beg, Robert Henderson

Proceedings Volume 11243, 112430S (2020) https://doi.org/10.1117/12.2544759

KEYWORDS: Luminescence, Flow cytometry, Microfluidics, Cameras, Particles, Fluorescence lifetime imaging, Visualization, Sensors, Cancer, Time correlated photon counting

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Proceedings Article | 4 March 2019 Presentation

Functional in vivo imaging using fluorescence lifetime light sheet microscopy (Conference Presentation)

Simon Ameer-Beg, Claire Mitchell, Simon Poland, Robert Knight, Guoqing Wang, Gerhard Holst

Proceedings Volume 10882, 108820J (2019) https://doi.org/10.1117/12.2509727

KEYWORDS: Microscopy, Luminescence, In vivo imaging, Fluorescence lifetime imaging, Cameras, Biological research, Statistical analysis, Microscopes, Phase shifts, Modulation

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Proceedings Article | 4 March 2019 Presentation

Single molecule imaging of fluorescence anisotropy (Conference Presentation)

Thomas Kavanagh, Viviane Devauges, Joseph Tideswell, Camille Perrin, Jin Li, Justin Aluko, Simon Poland, Andrew Beavil, Simon Ameer-Beg

Proceedings Volume 10884, 108840B (2019) https://doi.org/10.1117/12.2509832

KEYWORDS: Molecules, Fluorescence anisotropy, Fluorescence resonance energy transfer, Molecular interactions, Imaging systems, Real time imaging, Anisotropy, Energy transfer, Microscopes, Fluorescence spectroscopy

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Proceedings Article | 17 June 2013 Paper

Improving TCSPC data acquisition from CMOS SPAD arrays

Nikola Krstajić, Simon Poland, David Tyndall, Richard Walker, Simao Coelho, David Li, Justin Richardson, Simon Ameer-Beg, Robert Henderson

Proceedings Volume 8797, 879709 (2013) https://doi.org/10.1117/12.2032807

KEYWORDS: Cameras, Fluorescence lifetime imaging, Microscopes, Luminescence, Sensors, Field programmable gate arrays, Laser scanners, Data acquisition, Single photon, Image resolution

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Proceedings Article | 22 February 2013 Paper

Single molecule FRET using the FRET pair DRONPA/PhotoActivable mCherry

Viviane Devauges, Elena Ortiz-Zapater, Christina Efthymiou, Melanie Keppler, Jody Barbeau, Daniel Matthews, James Monypenny of Pitmilly, Paul Barber, Daniel Rolfe, Tony Ng, Simon Ameer-Beg

Proceedings Volume 8590, 85900A (2013) https://doi.org/10.1117/12.2002470

KEYWORDS: Fluorescence resonance energy transfer, Molecules, Luminescence, Fluorescence lifetime imaging, Ultraviolet radiation, Proteins, Switching, Molecular lasers, Quantum efficiency, Switches

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Proceedings Article | 22 February 2013 Paper

Development of a fast TCSPC FLIM-FRET imaging system

Simon Poland, Simao Coelho, Nikola Krstajić, David Tyndall, Richard Walker, James Monypenny, David Li, Robert Henderson, Simon Ameer-Beg

Proceedings Volume 8588, 85880X (2013) https://doi.org/10.1117/12.2004199

KEYWORDS: Fluorescence resonance energy transfer, Fluorescence lifetime imaging, Spatial light modulators, Luminescence, Sensors, Imaging systems, Microscopes, Cancer, Multiphoton microscopy, Single photon

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Proceedings Article | 22 February 2013 Paper

Multifocal multiphoton microscopy with adaptive optical correction

Simao Coelho, Simon Poland, Nikola Krstajic, David Li, James Monypenny, Richard Walker, David Tyndall, Tony Ng, Robert Henderson, Simon Ameer-Beg

Proceedings Volume 8588, 858817 (2013) https://doi.org/10.1117/12.2000188

KEYWORDS: Fluorescence lifetime imaging, Adaptive optics, Spatial light modulators, Multiphoton microscopy, Microscopy, Photons, Holograms, Optical calibration, Luminescence, Avalanche photodiodes

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Proceedings Article | 28 February 2011 Paper

Broadband coherent Raman imaging for multiplexed detection

Brad Littleton, Fred Festy, Simon Ameer-Beg, David Richards

Proceedings Volume 7903, 79032J (2011) https://doi.org/10.1117/12.875180

KEYWORDS: Raman spectroscopy, Multiplexing, Image segmentation, Coherence imaging, Molecules, Optical filters, Chromophores, Luminescence, CARS tomography, Image processing algorithms and systems

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Proceedings Article | 11 February 2011 Paper

Using adaptive optics for deep in-vivo multiphoton FLIM

Simon Poland, Gilbert Fruhwirth, Tony Ng, Simon Ameer-beg

Proceedings Volume 7903, 79032C (2011) https://doi.org/10.1117/12.875102

KEYWORDS: Adaptive optics, Fluorescence lifetime imaging, In vivo imaging, Fluorescence resonance energy transfer, Multiphoton microscopy, Microscopy, Wavefronts, Mirrors, Tissues, Remote sensing

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Proceedings Article | 11 February 2011 Paper

High-speed FRET screening for optical proteomics in a microfluidic format

Viput Visitkul, Daniel Matthews, Gregory Weitsman, Melanie Keppler, Simon Ameer-Beg

Proceedings Volume 7902, 79020P (2011) https://doi.org/10.1117/12.875510

KEYWORDS: Luminescence, Fluorescence resonance energy transfer, Microfluidics, Photons, Signal detection, Proteins, Green fluorescent protein, Cancer, Signal processing, Fluorescence lifetime imaging

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Proceedings Article | 13 February 2009 Paper

Deep-tissue multiphoton fluorescence lifetime microscopy for intravital imaging of protein-protein interactions

G. Fruhwirth, D. Matthews, A. Brock, M. Keppler, B. Vojnovic, T. Ng, S. Ameer-Beg

Proceedings Volume 7183, 71830L (2009) https://doi.org/10.1117/12.817129

KEYWORDS: Luminescence, In vivo imaging, Microscopy, Multiphoton fluorescence microscopy, Multiphoton microscopy, Fluorescence lifetime imaging, Receptors, Fluorescence resonance energy transfer, Microscopes, Tissues

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Proceedings Article | 5 March 2008 Paper

A high-content screening platform utilizing polarization anisotropy and FLIM microscopy

Daniel Matthews, Simon Ameer-Beg, Paul Barber, Glenn Pierce, Robert Newman, Boris Vojnovic, Leo Carlin, Melanie Keppler, Tony Ng, Klaus Suhling, Malcolm Irving

Proceedings Volume 6859, 685919 (2008) https://doi.org/10.1117/12.763310

KEYWORDS: Anisotropy, Fluorescence resonance energy transfer, Microscopy, Polarization, Luminescence, Fluorescence lifetime imaging, Proteins, Biosensors, Imaging systems, Microscopes

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Proceedings Article | 19 February 2007 Paper

Live cell tracking on an optical biochip platform

Kerenza Njoh, Paul Smith, Sally Chappell, Huw Summers, Daniel Matthews, David Morris, Andrew Goater, Julian Burt, Iestyn Pope, Boris Vojnovic, Simon Ameer-Beg, Rachel Errington

Proceedings Volume 6441, 64410X (2007) https://doi.org/10.1117/12.698935

KEYWORDS: Luminescence, Optical tracking, Quantum dots, Microscopy, Nanocrystals, Cancer, Microfluidics, Light emitting diodes, Tissues, Systems modeling

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Proceedings Article | 14 February 2007 Paper

A fluorescence biochip with a plasmon active surface

Daniel Matthews, Huw Summers, Kerenza Njoh, Sally Chappell, Rachel Errington, Paul Smith, Iestyn Pope, Paul Barber, Boris Vojnovic, Simon Ameer-Beg

Proceedings Volume 6450, 645006 (2007) https://doi.org/10.1117/12.698948

KEYWORDS: Luminescence, Metals, Surface plasmons, Molecules, Gold, Quantum dots, Glasses, Active optics, Microscopes, Line scan image sensors

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Proceedings Article | 23 February 2006 Paper

Scanning total internal reflection fluorescence imaging

A. Quirke, S. Ameer-Beg, M. Parsons, T. Ng, M. Irving, B. Vojnovic

Proceedings Volume 6089, 608907 (2006) https://doi.org/10.1117/12.648451

KEYWORDS: Signal detection, Luminescence, Interfaces, Point spread functions, Spatial filters, Fluorescence lifetime imaging, Light wave propagation, Microscopy, Wave propagation, Refractive index

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Proceedings Article | 23 February 2006 Paper

Mapping femtosecond pulse front distortion and group velocity dispersion in multiphoton microscopy

I. Tullis, S. Ameer-Beg, P. Barber, V. Rankov, B. Vojnovic

Proceedings Volume 6089, 60890Y (2006) https://doi.org/10.1117/12.646395

KEYWORDS: Dispersion, Distortion, Objectives, Cameras, Multiphoton microscopy, Microscopes, Femtosecond phenomena, Mirrors, Interferometry, Crystals

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Proceedings Article | 21 February 2006 Paper

Screening far red probes for use on optical biochip devices

Kerenza Njoh, Laurence Patterson, Klaus Pors, Mire Zloh, Simon Ameer-Beg, Huw Summers, Daniel Matthews, Rachel Errington, Paul Smith

Proceedings Volume 6088, 60880H (2006) https://doi.org/10.1117/12.664713

KEYWORDS: Luminescence, Absorption, Quantum efficiency, Imaging spectroscopy, Cell death, Green fluorescent protein, Absorbance, Biological research, Sensors, Picosecond phenomena

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Proceedings Article | 1 September 2005 Paper

Time-resolved fluorescence measurements using stroboscopic excitation

Daniel Matthews, Huw Summers, Kerenza Njoh, Rachel Errington, Paul Smith, Simon Ameer-Beg, Boris Vojnovic

Proceedings Volume 5864, 586409 (2005) https://doi.org/10.1117/12.632914

KEYWORDS: Luminescence, Semiconductor lasers, Molecules, Picosecond phenomena, Sensors, Bioalcohols, Absorption, Time resolved spectroscopy, Fluorescence resonance energy transfer, Aluminium gallium indium phosphide

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Proceedings Article | 28 April 2005 Paper

Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes

Daniel Matthews, Huw Summers, Kerenza Njoh, Rachel Errington, Paul Smith, Simon Ameer-Beg, Boris Vojnovic

Proceedings Volume 5705, (2005) https://doi.org/10.1117/12.590201

KEYWORDS: Luminescence, Sensors, Semiconductor lasers, Signal detection, Absorption, Pulsed laser operation, Time resolved spectroscopy, Bioalcohols, Streak cameras, Signal processing

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Proceedings Article | 30 March 2005 Paper

Dynamic imaging of protein-protein interactions by MP-FLIM

Simon Ameer-Beg, Marion Peter, Melanie Keppler, Soren Prag, Paul Barber, Tony Ng, Borivoj Vojnovic

Proceedings Volume 5700, (2005) https://doi.org/10.1117/12.589202

KEYWORDS: Fluorescence resonance energy transfer, Luminescence, Proteins, Fluorescence lifetime imaging, Fluorescent proteins, Photons, Quantum efficiency, Refractive index, Molecular interactions, Green fluorescent protein

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Proceedings Article | 30 March 2005 Paper

Global and pixel kinetic data analysis for FRET detection by multi-photon time-domain FLIM

Paul Barber, Simon Ameer-Beg, Julian Gilbey, Richard Edens, Ifeome Ezike, Borivoj Vojnovic

Proceedings Volume 5700, (2005) https://doi.org/10.1117/12.590510

KEYWORDS: Fluorescence resonance energy transfer, Data modeling, Fluorescence lifetime imaging, Luminescence, Photon counting, Proteins, Convolution, Molecules, Algorithm development, Data analysis

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Proceedings Article | 9 October 2003 Paper

Use of acceptor fluorescence for determining FRET lifetimes

Michael Hughes, Simon Ameer-Beg, Marion Peter, Tony Ng

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500641

KEYWORDS: Luminescence, Fluorescence resonance energy transfer, Photons, Molecules, Green fluorescent protein, Sensors, Quantum efficiency, Proteins, Fluorescence lifetime imaging, Photomultipliers

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Proceedings Article | 9 October 2003 Paper

Three-dimensional imaging and quantification of complex vascular networks

Paul Barber, Simon Ameer-Beg, Borivoj Vojnovic, Richard Hodgkiss, Gillian Tozer, John Wilson, Vivien Prise

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500540

KEYWORDS: 3D image processing, Fractal analysis, In vivo imaging, Point spread functions, 3D metrology, Microscopes, Hough transforms, Statistical analysis, Tissues, Cancer

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Proceedings Article | 9 October 2003 Paper

Imaging protein-protein interactions by multiphoton FLIM

Simon Ameer-Beg, Natasha Edme, Marion Peter, Paul Barber, Tony Ng, Borivoj Vojnovic

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500544

KEYWORDS: Fluorescence resonance energy transfer, Proteins, Luminescence, Biosensors, Fluorescence lifetime imaging, Fluorescent proteins, Imaging spectroscopy, Green fluorescent protein, Molecules, Data modeling

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Proceedings Article | 9 October 2003 Paper

Time-resolved multiphoton imaging of the interaction between the PKC and the NFkappaB cell signalling pathways

Penny Morton, Tony Ng, Sarah Roberts, Borivoj Vojnovic, Simon Ameer-Beg

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500513

KEYWORDS: Fluorescence resonance energy transfer, Luminescence, Fluorescence lifetime imaging, Proteins, Confocal microscopy, Photons, Microscopy, Receptors, Control systems, Cancer

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