Mr. Sungho Kim Profile

Sungho Kim

Researcher at Ulsan National Institute of Science and Technology

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Proceedings Article | 11 March 2015 Paper

Correlative super-resolution fluorescence microscopy combined with optical coherence microscopy

Sungho Kim, Gyeongtae Kim, Soohyun Jang, Sang-Hee Shim, Sung Chul Bae

Proceedings Volume 9316, 93160R (2015) https://doi.org/10.1117/12.2079340

KEYWORDS: Microscopes, Luminescence, Microscopy, Super resolution, Tissues, Adaptive optics, Brain, Optical microscopy, Optical coherence microscopy, Imaging systems

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Recent development of super-resolution fluorescence imaging technique such as stochastic optical reconstruction microscopy (STORM) and photoactived localization microscope (PALM) has brought us beyond the diffraction limits. It allows numerous opportunities in biology because vast amount of formerly obscured molecular structures, due to lack of spatial resolution, now can be directly observed. A drawback of fluorescence imaging, however, is that it lacks complete structural information. For this reason, we have developed a super-resolution multimodal imaging system based on STORM and full-field optical coherence microscopy (FF-OCM). FF-OCM is a type of interferometry systems based on a broadband light source and a bulk Michelson interferometer, which provides label-free and non-invasive visualization of biological samples. The integration between the two systems is simple because both systems use a wide-field illumination scheme and a conventional microscope. This combined imaging system gives us both functional information at a molecular level (~20nm) and structural information at the sub-cellular level (~1μm). For thick samples such as tissue slices, while FF-OCM is readily capable of imaging the 3D architecture, STORM suffer from aberrations and high background fluorescence that substantially degrade the resolution. In order to correct the aberrations in thick tissues, we employed an adaptive optics system in the detection path of the STORM microscope. We used our multimodal system to obtain images on brain tissue samples with structural and functional information.

Proceedings Article | 11 March 2015 Paper

Real-time Raman sensing without spectrometer

Min Ju Kim, Sungho Kim, Timothy Yang, Dinesh Kumar, Sung Chul Bae

Proceedings Volume 9318, 93180Z (2015) https://doi.org/10.1117/12.2079278

KEYWORDS: Raman spectroscopy, Modulation, Spectroscopy, Optical filters, Molecules, Sensors, Radon, Molecular lasers, Bioalcohols, Semiconductor lasers

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Raman spectroscopy has been a powerful tool in various fields of science and technology ranging from analytical chemistry to biomedical imaging. In spite of unique features, Raman spectroscopy has also some limitations. Among them are weak Raman signal compared to strong fluorescence and relatively complicated setup with expensive and bulky spectrometer. In order to increase the sensitivity of Raman technique, many clever attempts have been made and some of them were very successful including CARS, SRS, and so on. However, these still requires expensive and more complicated setup. In this work, we have attempted to build a real-time compact Raman sensor without spectrometer. Conventional spectrometer was replaced with a narrow-band optical filter and alternatively modulated two lasers with slightly different wavelengths. At one laser, Raman signal from a target molecule was transmitted through the optical filter. At the other laser, this signal was blocked by the optical filter and could not be detected by photon detector. The alternative modulation of two lasers will modulate the Raman signal from a target molecule at the same modulation frequency. This modulated weak Raman signal was amplified by a lock-in amplifier. The advantages of this setup include compactness, low cost, real-time monitoring, and so on. We have tested the sensitivity of this setup and we found that it doesn’t have enough sensitivity to detect single molecule-level, but it is still good enough to monitor the change of major chemical composition in the sample.

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