KEYWORDS: In vivo imaging, Stem cells, Brain, Nanoparticles, Luminescence, Alzheimer's disease, Magnetic resonance imaging, Imaging systems, Biology, Animal model studies
Stem cell therapy is a promising tool for the treatment of diverse conditions, including neurodegenerative diseases such as Alzheimer’s disease (AD). To understand transplanted stem cell biology, in vivo imaging is necessary. Nanomaterial has great potential for in vivo imaging and several noninvasive methods are used, such as magnetic resonance imaging, positron emission tomography, fluorescence imaging (FI) and near-infrared FI. However, each method has limitations for in vivo imaging. To overcome these limitations, multimodal nanoprobes have been developed. In the present study, we intravenously injected human adipose-derived stem cells (hASCs) that were labeled with a multimodal nanoparticle, LEO-LIVE™-Magnoxide 675 or 797 (BITERIALS, Seoul, Korea), into Tg2576 mice, an AD mouse model. After sequential in vivo tracking using Maestro Imaging System, we found fluorescence signals up to 10 days after injection. We also found strong signals in the brains extracted from hASC-transplanted Tg2576 mice up to 12 days after injection. With these results, we suggest that in vivo imaging with this multimodal nanoparticle may provide a useful tool for stem cell tracking and understanding stem cell biology in other neurodegenerative diseases.
Stem cell therapy is a promising tool for the treatment of diverse conditions including neurodegenerative diseases. To
understand transplanted stem cell biology, in vivo imaging is necessary. Nano material has great potential for in vivo
imaging and several noninvasive methods are used such as magnetic resonance imaging (MRI), positron emission
tomography (PET), Fluorescence imaging (FI) and Near-infrared fluorescence imaging (NIRFI). However, each method
has limitations for in vivo imaging. To overcome these limitations, multimodal nanoprobes have been developed. In the
present study, we intravenously injected human adipose derived stem cells (hASCs) that labeled with multimodal nano
particle, LEO-LIVETM-Magnoxide 797 or 675, into the Tg2576 mice, Alzheimer’s disease (AD) mouse model.
Sequential in vivo tracking was performed with mice injected with hASCs. We could found fluorescence signals until 10
days after injection.
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