Mr. Sven Delbeck Profile

Sven Delbeck

SPIE Involvement:

Author

Area of Expertise:

Vibrational Spectroscopy , Analytical Chemistry , Chromatography , Photometry , Chemometrics , Data Analytics

Publications (9)

SPIE Journal Paper | 8 March 2021 Open Access

Systematic stability testing of insulins as representative biopharmaceuticals using ATR FTIR-spectroscopy with focus on quality assurance

Sven Delbeck, Herbert Michael Heise

JBO, Vol. 26, Issue 04, 043007, (March 2021) https://doi.org/10.1117/12.10.1117/1.JBO.26.4.043007

KEYWORDS: Proteins, Deconvolution, Infrared spectroscopy, Statistical analysis, Spectroscopy, Principal component analysis, Absorbance, Biological research, Testing and analysis, Data analysis

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Significance: Bioactive proteins represent the most important component class in biopharmaceutical products for therapeutic applications. Their production is most often biotechnologically realized by genetically engineered microorganisms. For the quality assurance of insulins as representatives of life-saving pharmaceuticals, analytical methods are required that allow more than total protein quantification in vials or batches. Chemical and physical factors such as unstable temperatures or shear rate exposure under storage can lead to misfolding, nucleation, and subsequent fibril forming of the insulins. The assumption is valid that these processes go parallel with a decrease in bioactivity. Aim: Infrared (IR) spectroscopy has been successfully utilized for secondary structure analysis in cases of protein misfolding and fibril formation. Approach: A reliable method for the quantification of the secondary structure changes has been developed using insulin dry-film Fourier-transform IR spectroscopy in combination with the attenuated total reflection (ATR) technique and subsequent data analyses such as band-shift determination, spectral band deconvolution, and principal component analysis. Results: A systematic study of insulin spectra was carried out on model insulin specimens, available either as original formulations or as hormones purified by ultrafiltration. Insulin specimens were stored at different temperatures, i.e., 0°C, 20°C, and 37°C, respectively, for up to three months. Weekly ATR-measurements allowed the monitoring of hormone secondary structure changes, which are supposed to be negatively correlated with insulin bioactivity. Conclusions: It could be shown that IR-ATR spectroscopy offers a fast and reliable analytical method for the determination of secondary structural changes within insulin molecules, as available in pharmaceutical insulin formulations and therefore challenges internationally established measurement techniques for quality control regarding time, costs, and effort of analysis.

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Proceedings Article | 21 February 2020 Paper

Molecular monitoring of different insulins using FTIR-ATR spectroscopy for pharmaceutical quality control

S. Delbeck, H. M. Heise

Proceedings Volume 11236, 112360V (2020) https://doi.org/10.1117/12.2546170

KEYWORDS: Proteins, Spectroscopy, Deconvolution, Infrared spectroscopy, Biological research, Infrared radiation, Absorbance, Principal component analysis, Molecular spectroscopy, Attenuated total reflectance

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Proceedings Article | 21 February 2020 Paper

Development of an insulin activity test based on cell culture monitoring by microdialysis and infrared spectrometry

A. Krolinski, S. Delbeck, Y. Dederich, S. Stoppelkamp, H. M. Heise

Proceedings Volume 11236, 112360O (2020) https://doi.org/10.1117/12.2546154

KEYWORDS: Glucose, Infrared spectroscopy, Proteins, Biological research, Testing and analysis

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Proceedings Article | 27 February 2019 Paper

Applications of tapered flat silver halide fibers chemically modified by 12-mercaptododecanoic acid NHS ester for infrared biospectroscopy with prospects for medical diagnostics

S. Delbeck, B. Schmitz, A. Nabers, K. Gerwert, A. Habermehl, U. Lemmer, H. M. Heise

Proceedings Volume 10872, 108720T (2019) https://doi.org/10.1117/12.2511023

KEYWORDS: Optical fibers, Printing, Proteins, Infrared radiation, Nanoparticles, Sensors, Absorption

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Proceedings Article | 20 February 2018 Paper

Evaluation and benchmarking of an EC-QCL-based mid-infrared spectrometer for monitoring metabolic blood parameters in critical care units

M. Grafen, S. Delbeck, H. Busch, H. Heise, A. Ostendorf

Proceedings Volume 10501, 105010A (2018) https://doi.org/10.1117/12.2289625

KEYWORDS: Spectroscopy, Glucose, Quantum cascade lasers, Infrared spectroscopy, FT-IR spectroscopy, Blood, Calibration, Mid-IR, Infrared radiation

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Proceedings Article | 13 February 2018 Presentation + Paper

Applications of tapered flat silver halide fiber elements for infrared biospectroscopy with aspects of optical stability and biocompatibility

Sven Delbeck, Lukas Küpper, Herbert Heise

Proceedings Volume 10488, 104880Q (2018) https://doi.org/10.1117/12.2290967

KEYWORDS: Silver, Glucose, Sensors, Chemical elements, Fiber optics sensors, Spectroscopy, Blood, Absorbance, Infrared radiation, Infrared spectroscopy

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Proceedings Article | 17 February 2017 Presentation + Paper

Reliable glucose monitoring by ex-vivo blood microdialysis and infrared spectrometry for patients in critical care

Thorsten Vahlsing, Sven Delbeck, Janpeter Budde, Dieter Ihrig, Steffen Leonhardt, H. Michael Heise

Proceedings Volume 10072, 100720D (2017) https://doi.org/10.1117/12.2253086

KEYWORDS: Infrared spectroscopy, Glucose, Blood, Infrared radiation, Spectroscopy, Spectral calibration, Calibration, Sensing systems, Infrared sensors, Biosensors, Absorbance, Statistical analysis, Microfluidics, Sensors

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Proceedings Article | 7 March 2016 Presentation + Paper

Combination of micro-dialysis and infrared spectroscopy: a multianalyte assay for accurate biofluid analysis and patient monitoring

Thorsten Vahlsing, Sven Delbeck, Janpeter Budde, Dieter Ihrig, H. Michael Heise

Proceedings Volume 9704, 97040R (2016) https://doi.org/10.1117/12.2214636

KEYWORDS: Infrared spectroscopy, Glucose, Biological research, Spectroscopy, Calibration, Blood, Quantitative analysis, Clinical research, Absorbance, Microfluidics, Sensors, Sodium

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Proceedings Article | 9 March 2015 Paper

Ex-vivo glucose sensors using micro-dialysis: importance of on-line recovery rate determination by multi-analyte infrared spectrometry

Thorsten Vahlsing, Sven Delbeck, Janpeter Budde, Lars Cocchieri, Dieter Ihrig, Steffen Leonhardt, H. Heise

Proceedings Volume 9332, 933209 (2015) https://doi.org/10.1117/12.2080289

KEYWORDS: Glucose, Infrared spectroscopy, Blood, Microfluidics, Spectroscopy, Optical filters, Absorbance, Spectral resolution, Infrared radiation, Sensors

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Showing 5 of 9 publications

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