Coherent anti-Stokes Raman scattering (CARS) microscopy, which can produce images of specific molecules without
staining, has attracted the attention of researchers, as it matches the need for molecular imaging and pathway analysis of
live cells. In particular, there have been an increasing number of CARS experimental results regarding lipids in live cells,
which cannot be fluorescently tagged while keeping the cells alive. One of the important applications of lipid research is
for the metabolic syndrome. Since the metabolic syndrome is said to be related to the lipids in lipocytes, blood, arterial
vessels, and so on, the CARS technique is expected to find application in this field. However, CARS microscopy
requires a pair of picosecond laser pulses, which overlap both temporally and spatially. This makes the optical
adjustments of a CARS microscope challenging. The authors developed a CARS unit that includes optics for easy and
stable adjustment of the overlap of these laser pulses. Adding the CARS unit to a laser scanning microscope provides
CARS images of a high signal-to-noise ratio, with an acquisition rate as high as 2 microseconds per pixel. Thus, images
of fast-moving lipid droplets in Hela cells were obtained.
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