Prof. Tatiana Globus Profile

Prof. Tatiana Globus

Principal Scientist/RA Professor at Univ of Virginia

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Publications (18)

Proceedings Article | 31 May 2013 Paper

Sub-terahertz resonance spectroscopy of biological macromolecules and cells

Tatiana Globus, Aaron Moyer, Boris Gelmont, Tatyana Khromova, Igor Sizov, Jerome Ferrance

Proceedings Volume 8716, 87160N (2013) https://doi.org/10.1117/12.2016108

KEYWORDS: Spectroscopy, Absorption, Terahertz radiation, Sensors, Macromolecules, Proteins, Spectroscopes, Spectral resolution, Bacteria, Molecular spectroscopy

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Proceedings Article | 29 May 2013 Paper

Portable sub-terahertz resonance spectrometer combined with microfluidic sample cell

Jerome Ferrance, Alexander Khromov, Aaron Moyer, Tatiana Khromova, Boris Gelmont, Igor Sizov, Tatiana Globus

Proceedings Volume 8726, 87260I (2013) https://doi.org/10.1117/12.2015811

KEYWORDS: Copper, Photoresist materials, Metals, Gold, Spectroscopy, Microfluidics, Positron emission tomography, Terahertz radiation, Silver, Liquids

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Proceedings Article | 27 April 2010 Paper

Low THz spectroscopy of some widely used explosives

Tatiana Globus, Boris Gelmont, Tatyana Khromova

Proceedings Volume 7671, 76710C (2010) https://doi.org/10.1117/12.849921

KEYWORDS: Terahertz radiation, Explosives, Spectroscopy, Solids, Liquids, Absorption, Magnesium, Spectral resolution, Water, Beam splitters

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Proceedings Article | 26 September 2007 Paper

Low-terahertz spectroscopy of liquid water

T. Globus, A. Bykhovski, T. Khromova, B. Gelmont, L. Tamm, L. Salay

Proceedings Volume 6772, 67720S (2007) https://doi.org/10.1117/12.739859

KEYWORDS: Absorption, Liquids, Terahertz radiation, Spectroscopy, Hydrogen, Molecules, Water, Chemical species, Absorbance, Oxygen

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Proceedings Article | 19 May 2006 Paper

THz resonance spectra of Bacillus Subtilis cells and spores in PE pellets and dilute water solutions

Tatiana Globus, Tatyana Khromova, Dwight Woolard, Alan Samuels

Proceedings Volume 6212, 62120K (2006) https://doi.org/10.1117/12.665246

KEYWORDS: Terahertz radiation, Absorption, Matrices, Infrared spectroscopy, Genetics, Water, Refractive index, Magnesium, Fabry–Perot interferometers, Spectroscopy

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Proceedings Article | 19 May 2006 Paper

An analysis of the THz frequency signatures in the cellular components of biological agents

Alexei Bykhovski, Tatiana Globus, Tatyana Khromova, Boris Gelmont, Dwight Woolard, Maria Bykhovskaia

Proceedings Volume 6212, 62120H (2006) https://doi.org/10.1117/12.665272

KEYWORDS: Terahertz radiation, Molecules, Absorption, Biological research, Biological weapons, Water, Chemical species, Computer simulations, Data modeling, Solids

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Proceedings Article | 27 February 2006 Paper

Terahertz characterization of dilute solutions of DNA

Tatiana Globus, Tatyana Khromova, Boris Gelmont, Dwight Woolard, Lukas Tamm

Proceedings Volume 6093, 609308 (2006) https://doi.org/10.1117/12.646529

KEYWORDS: Terahertz radiation, Absorption, Spectroscopy, Absorbance, Molecules, Proteins, Molecular interactions, FT-IR spectroscopy, Liquids, Fourier transforms

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Only few studies have attempted to characterize biological materials by THz spectroscopy. Most of these used either solid samples or biological tissues. In this work, we present results of THz spectroscopic characterization of dilute solutions of DNA samples. Water and heavy water (D₂O) have strong absorbance that overlap significantly with important absorption bands of biomolecules in conventional FTIR spectroscopy. Cumbersome spectral subtractions and highly concentrated samples are therefore required to partially overcome problems of water interference in FTIR spectra of biomolecules. Although liquid water absorbs and contributes to background in the THz spectral range of interest, the level of water absorption in the low THz range is at least 2.5 orders of magnitude less than in the far IR. Here, we demonstrate that reproducible spectra of dilute solutions of DNA in the frequency range 10-24 cm^-1 can be obtained. We show that dilute aqueous samples of DNA produce THz spectra with signals that do not overlap with those of water. This is a significant achievement towards the goal of developing THz resonance spectroscopy as a useful tool for the biological sciences because all biological functions of DNA and proteins take place in aqueous environments. A simple technique for sample preparation and characterization is described. Samples containing as little as 100 ng of DNA in 10 μl of water (0.01 mg/ml or 0.001%) have been prepared and measured. The signal/noise ratios of THz spectra of these samples are sufficient to detect reproducible resonances at several characteristic frequencies. The effect of orientation on different substrates and the mechanism of sensitivity enhancement in these samples is discussed. It should be possible to extend these methods to also study proteins in dilute solutions. Advantages of using dilute samples include small quantities of biological material required, the absence of interference from interactions between neighboring molecules, and the absence of problems with light-scattering that are often encountered with short wave-length optical techniques.

Proceedings Article | 10 February 2006 Paper

Vertical-cavity laser based on interband-tunneling staggered-bandgap heterostructure

Boris Gelmont, Dwight Woolard, Tatiana Globus

Proceedings Volume 6132, 61320E (2006) https://doi.org/10.1117/12.646882

KEYWORDS: Indium arsenide, Mid-IR, Mirrors, Heterojunctions, Quantum wells, Optical simulations, Laser development, Electron transport, Dielectrics, Instrument modeling

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Proceedings Article | 5 November 2005 Paper

THz absorption signature detection of genetic material of E. coli and B. subtilis

Alexei Bykhovski, Xiaowei Li, Tatiana Globus, Tatyana Khromova, Boris Gelmont, Dwight Woolard, Alan Samuels, James Jensen

Proceedings Volume 5995, 59950N (2005) https://doi.org/10.1117/12.629959

KEYWORDS: Terahertz radiation, Absorption, Genetics, Biological weapons, Proteins, Spectroscopy, Biological research, Computer simulations, Data modeling, Solids

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The development of efficient biological agent detection techniques requires in-depth understanding of THz absorption spectral features of different cell components. Chromosomal DNA, RNAs, proteins, bacterial cell wall, proteinaceous coat might be essential for bacterial cells and spores THz signature. As a first step, the DNA's contribution into entire cell THz spectra was analyzed. The experimental study of cells and DNAs of E. coli and cells/spores and DNA of Bacillus subtilis was conducted. Samples were prepared in the form of water solutions (suspension) with the concentrations in the range 0.01-1 mg/ml. The measurable difference in the THz transmission spectra of E. coli and Bacillus subtilis DNAs was observed. The correlation between chromosomal DNA signature and a corresponding entire spore/cell signature was observed. This correlation was especially pronounced for spores of Bacillus subtilis and their DNA. These experimental results justify our approach to develop a model for THz signatures of biological simulants and agents. In parallel with the experimental study, for the first time, the computer modeling and simulation of chromosome DNAs of E. coli and Bacillus subtilis was performed and their THz signatures were calculated. The DNA structures were optimized using the Amber software package. Also, we developed the initial model of the DNA fragment poly(dAT)-poly(dTA) solvated in water to be used in the simulations of genetic material (DNA and RNA) of spores and cells. Molecular dynamical simulations were conducted using explicit solvent (3-point TIP3P water) and implicit solvent (generalized Born) models. The calculated THz signatures of E. coli and Bacillus subtilis DNAs and poly(dAT)-poly(dTA) reproduce many features of our measured spectra. The results of this study demonstrate that THz Fourier transform infrared spectroscopy is a promising tool in generating spectral data for complex biological objects such as bacterial cells and spores.

Proceedings Article | 18 May 2005 Paper

Terahertz signature characterization of bio-simulants

Alexander Majewski, Peter Miller, Rene Abreu, Jeffrey Grotts, Tatiana Globus, Elliott Brown

Proceedings Volume 5790, (2005) https://doi.org/10.1117/12.603658

KEYWORDS: Absorption, Aerosols, Terahertz radiation, Spectroscopy, Filtering (signal processing), Fourier transforms, Optical filters, Biological weapons, Signal processing, Electronic filtering

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Proceedings Article | 18 May 2005 Paper

A bio-molecular inspired electronic architecture: bio-based device concepts for enhanced sensing

Dwight Woolard, Ying Luo, Boris Gelmont, Tatiana Globus, James Jensen

Proceedings Volume 5790, (2005) https://doi.org/10.1117/12.601029

KEYWORDS: Terahertz radiation, Molecules, Absorption, Biosensing, Phonons, Sensors, Computer architecture, Spectroscopy, Electronic components, Telecommunications

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Proceedings Article | 18 May 2005 Paper

THz characterization of lysozyme at different conformations

Tatiana Globus, Tatyana Khromova, Rebecca Lobo, Dwight Woolard, Nathan Swami, Erik Fernandez

Proceedings Volume 5790, (2005) https://doi.org/10.1117/12.603581

KEYWORDS: Proteins, Terahertz radiation, Liquids, Spectroscopy, Absorption, Hydrogen, FT-IR spectroscopy, Molecules, Circular dichroism spectroscopy, Solids

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Proceedings Article | 1 April 2005 Paper

Terahertz spectroscopic characterization of cancer cells

Tatiana Globus, Dan Theodorescu, Henry Frierson, Tatyana Khromova, Dwight Woolard

Proceedings Volume 5692, (2005) https://doi.org/10.1117/12.594391

KEYWORDS: Cancer, Bladder cancer, Tumors, Liquids, Terahertz radiation, Spectroscopy, Absorption, Prostate cancer, Prostate, FT-IR spectroscopy

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Proceedings Article | 14 December 2004 Paper

Optical characteristics of biological molecules in the terahertz gap

Tatiana Globus, Ramakrishnan Parthasarathy, Tatyana Khromova, Dwight Woolard, Nathan Swami, Andrew Gatesman, Jerry Waldman

Proceedings Volume 5584, (2004) https://doi.org/10.1117/12.580838

KEYWORDS: Absorption, Refractive index, Liquids, Reflection, Molecules, Spectroscopy, Terahertz radiation, Data modeling, Biomedical optics, Molecular biology

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Proceedings Article | 8 September 2004 Paper

Terahertz signatures of biological-warfare-agent simulants

Tatiana Globus, Dwight Woolard, Tatyana Khromova, Ramakrishnan Partasarathy, Alexander Majewski, Rene Abreu, Jeffrey Hesler, Shing-Kuo Pan, Geoff Ediss

Proceedings Volume 5411, (2004) https://doi.org/10.1117/12.549128

KEYWORDS: Spectroscopy, FT-IR spectroscopy, Liquids, Terahertz radiation, Mercury, Lamps, Radio astronomy, Observatories, Bacteria, Error analysis

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Proceedings Article | 27 February 2004 Paper

Terahertz Fourier transform characterization of biological materials in solid and liquid phases

Tatiana Globus, Tatiana Khromova, Dwight Woolard, Boris Gelmont

Proceedings Volume 5268, (2004) https://doi.org/10.1117/12.519172

KEYWORDS: Liquids, Solids, Absorption, Molecules, Spectroscopy, Terahertz radiation, Molecular biology, Fourier transforms, Macromolecules, Proteins

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Proceedings Article | 7 February 2002 Paper

Far-infrared phonon modes of selected RNA molecules

Tatiana Globus, Maria Bykhovskaia, Boris Gelmont, Dwight Woolard

Proceedings Volume 4574, (2002) https://doi.org/10.1117/12.455149

KEYWORDS: Molecules, Absorption, Phonons, Oscillators, FT-IR spectroscopy, Spectroscopy, Macromolecules, Hydrogen, Far infrared, Potassium

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Proceedings Article | 13 November 1998 Paper

Fabrication and test results for integrated submillimeter-wavelength planar Schottky mixers on quartz utilizing MASTER

Steven Marazita, William Bishop, Kai Hui, Jeffrey Hesler, Roland Feinaugle, Tatiana Globus, Thomas Crowe

Proceedings Volume 3465, (1998) https://doi.org/10.1117/12.331167

KEYWORDS: Etching, Quartz, Semiconducting wafers, Gallium arsenide, Diodes, Wafer bonding, Gold, Wet etching, Heterodyning, Optical alignment

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Showing 5 of 18 publications

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