Dr. Thomas M. Jovin Profile

Dr. Thomas M. Jovin

at Max-Planck-Institut für Biophysikalische Chemie

SPIE Involvement:

Author

Proceedings Article | 10 March 2015 Paper

Generation 3 programmable array microscope (PAM) for high speed large format optical sectioning in fluorescence

Anthony de Vries, Nathan Cook, Stephan Kramer, Donna Arndt-Jovin, Thomas Jovin

Proceedings Volume 9376, 93760C (2015) https://doi.org/10.1117/12.2076390

KEYWORDS: Digital micromirror devices, Cameras, Microscopes, Luminescence, Diffraction, Optical filters, Optical lithography, Signal to noise ratio, Receptors, Light sources

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We report on the current version of the optical sectioning programmable array microscope (PAM) implemented with a single digital micro-mirror device (DMD) spatial light modulator utilized as a mask in both the fluorescence excitation and emission paths. The PAM incorporates structured illumination and structured detection operating in synchrony. A sequence of binary patterns of excitation light in high definition format (1920×1080 elements) is projected into the focal plane of the microscope at the 18 kHz binary frame rate of the Texas Instruments 1080p DMD. The resulting fluorescent emission is captured as two distinct signals: conjugate (c, ca. “on-focus”) consisting of light impinging on and deviated from the “on” elements of the DMD, and the non-conjugate (nc, ca. “out-of-focus”) light falling on and deviated from the “off” elements. The two distinct, deflected beams are optically filtered and detected either by two individual cameras or captured as adjacent images on a single camera after traversing an image combiner. The sectioned image is gained from a subtraction of the nc image from the c image, weighted in accordance with the pattern(s) used for illumination and detection and the relative exposure times of the cameras. The widefield image is given by the sum of the c and nc images. This procedure allows a high duty cycle (typically 25-50%) of on-elements in the excitation patterns and thus functions with low light intensities, preventing saturation and minimizing photobleaching of sensitive fluorophores. The corresponding acquisition speed is also very high, limited only by the bandwidth of the camera(s) (100 fps full frame with the sCMOS camera in current use) and the optical power of the light source (lasers, large area LEDs). In contrast to the static patterns typical of SIM systems, the programmable array allows optimization of the patterns (duty cycle, feature size and distribution), thus enabling a wide range of applications, ranging from patterned photobleaching, (e.g., FRAP, FLIP) and photoactivation, spatial superresolution, automated adaptive tracking and minimization of light exposure (MLE), and photolithography.

Proceedings Article | 15 February 2011 Paper

Generation-3 programmable array microscope (PAM) with digital micro-mirror device (DMD)

Pieter A. A. De Beule, Anthony H. B. de Vries, Donna Arndt-Jovin, Thomas Jovin

Proceedings Volume 7932, 79320G (2011) https://doi.org/10.1117/12.879611

KEYWORDS: Digital micromirror devices, Luminescence, Microscopes, Diffraction, Imaging systems, Glasses, Optical design, Spatial light modulators, Mirrors, Receptors

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Proceedings Article | 3 March 2009 Paper

Interplay of multivalency and optical properties of quantum dots: implications for sensing and actuation in living cells

Guillermo Menéndez, María Julia Roberti, Valeria Sigot, María Etchehon, Thomas Jovin, Elizabeth Jares-Erijman

Proceedings Volume 7189, 71890P (2009) https://doi.org/10.1117/12.816896

KEYWORDS: Molecules, Proteins, Luminescence, Nanosensors, Molecular aggregates, Sensors, Quantum dots, Nanoparticles, Fluorescence resonance energy transfer, Absorption

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Proceedings Article | 31 March 2007 Paper

Biological applications of an LCoS-based programmable array microscope (PAM)

Guy Hagen, Wouter Caarls, Martin Thomas, Andrew Hill, Keith Lidke, Bernd Rieger, Cornelia Fritsch, Bert van Geest, Thomas Jovin, Donna Arndt-Jovin

Proceedings Volume 6441, 64410S (2007) https://doi.org/10.1117/12.710995

KEYWORDS: Microscopes, Luminescence, Spatial light modulators, Fluorescence lifetime imaging, Receptors, Image processing, Liquid crystal on silicon, Molecules, Confocal microscopy, Diffusion

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Proceedings Article | 27 March 2006 Paper

Spectroscopic modulation of multifunctionalized quantum dots for use as biological probes and effectors

Sandra Miskoski, Luciana Giordano, Maria Etchehon, Guillermo Menendez, Keith Lidke, Guy Hagen, Thomas Jovin, Elizabeth Jares-Erijman

Proceedings Volume 6096, 60960X (2006) https://doi.org/10.1117/12.649835

KEYWORDS: Fluorescence resonance energy transfer, Quantum dots, Modulation, Luminescence, Nanoparticles, Spectroscopy, Ultraviolet radiation, Visible radiation, Absorption, Imaging spectroscopy

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Proceedings Article | 27 March 2006 Paper

In vivo cell imaging with semiconductor quantum dots and noble metal nanodots

Donna Arndt-Jovin, M. Arturo Lopez-Quintela, Diane Lidke, María Rodríguez, Francisco Martinez Santos, Keith Lidke, Guy Hagen, Thomas Jovin

Proceedings Volume 6096, 60960P (2006) https://doi.org/10.1117/12.646794

KEYWORDS: Gold, Receptors, Quantum dots, Semiconductors, Luminescence, Particles, In vivo imaging, Proteins, Metals, Electrodes

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Proceedings Article | 29 March 2005 Paper

Adaptation of nanoarrays for the study of alpha-synuclein aggregation: preliminary results

Heidelinde Dietrich, Richard van den Doel, Wolfgang Hoyer, Wim van Oel, Guus Liqui Lung, Yuval Garini, Thomas Jovin, Ian Young

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.587493

KEYWORDS: Humidity, Proteins, Temperature metrology, Microscopes, CCD cameras, Luminescence, Liquids, Calibration, Signal detection, Objectives

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Proceedings Article | 21 June 2004 Paper

Photochromic fluorescence resonance energy transfer (pcFRET): formalism, implementation, and perspectives

Elizabeth Jares-Erijman, Luciana Giordano, Carla Spagnuolo, Jennifer Kawior, Rudolph Vermeij, Thomas Jovin

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.527742

KEYWORDS: Fluorescence resonance energy transfer, Absorption, Luminescence, Visible radiation, Near ultraviolet, Microscopy, Absorbance, Modulation, Quantum efficiency, Spectroscopy

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Proceedings Article | 21 June 2004 Open Access

Dynamic and static fluorescence anisotropy in biological microscopy (rFLIM and emFRET)

Thomas Jovin, Diane Lidke, Janine Post

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.528469

KEYWORDS: Anisotropy, Venus, Luminescence, Proteins, Fluorescence resonance energy transfer, Fluorescence anisotropy, Microscopy, Molecules, Absorption, Data modeling

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Proceedings Article | 10 April 1997 Paper

Super-resolution MAP algorithms applied to fluorescence imaging

Peter Verveer, Geert van Kempen, Thomas Jovin

Proceedings Volume 2984, (1997) https://doi.org/10.1117/12.271258

KEYWORDS: Expectation maximization algorithms, Confocal microscopy, Luminescence, Image restoration, Algorithm development, Microscopy, Super resolution, Computer simulations, Optical spheres, Optical simulations

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Proceedings Article | 17 August 1994 Paper

Lifetime-resolved fluorescence imaging

Robert Clegg, T. Gadella, Thomas Jovin

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182715

KEYWORDS: Luminescence, Modulation, Phase shift keying, Microchannel plates, Photolysis, Microscopes, Statistical analysis, Image intensifiers, Heterodyning, CCD cameras

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Proceedings Article | 1 April 1992 Paper

Time-resolved imaging fluorescence microscopy

Robert Clegg, Brett Feddersen, Enrico Gratton, Thomas Jovin

Proceedings Volume 1640, (1992) https://doi.org/10.1117/12.58237

KEYWORDS: Luminescence, Modulation, Phase shift keying, Microscopes, Homodyne detection, Microchannel plates, CCD cameras, Heterodyning, Image intensifiers, Sensors

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Proceedings Article | 1 December 1991 Paper

Digital imaging microscopy: the marriage of spectroscopy and the solid state CCD camera

Thomas Jovin, Donna Arndt-Jovin

Proceedings Volume 1439, (1991) https://doi.org/10.1117/12.50457

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Proceedings Article | 1 June 1991 Paper

Rotational diffusion of receptors for epidermal growth factor measured by time-resolved phosphorescence depolarization

Raphael Zidovetzki, David Johnson, Donna Arndt-Jovin, Thomas Jovin

Proceedings Volume 1432, (1991) https://doi.org/10.1117/12.44211

KEYWORDS: Receptors, Phosphorescence, Diffusion, Picosecond phenomena, Proteins, Fluorescence anisotropy, Tissues, Anisotropy, Spectroscopy, Optical filters

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