Dr. Tim J. Abram Profile

Dr. Tim J. Abram

Director of R&D at Velox Biosystems

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Proceedings Article | 18 February 2010 Paper

A PDMS sample pretreatment microdevice to enable downstream electrokinetic manipulations in bovine serum

Timothy Abram, David Clague

Proceedings Volume 7593, 759318 (2010) https://doi.org/10.1117/12.846448

KEYWORDS: Microfluidics, Lab on a chip, Diagnostics, Electrodes, Statistical analysis, Blood, Proteins, Biological research, Video, Capillaries

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The notion of sample preconditioning, or pretreatment, as a micro-unit operation in a Lab on a Chip (LOC) system has yet to be realized in commercial practice. As is well known, Biomarker detection in complex, biological samples, such as blood, requires a series of pretreatment steps to enable detection of specific markers. On chip, such a process usually relies on "off-chip" sample pretreatment prior to "on-chip" analyte manipulations and detection. Presented in this paper is a PDMS, pretreatment chip based on the design of Oddy et al.1 with a view to enable a self-contained LOC platform. The chip was designed to directly manipulate the suspended species while adjusting fluid properties using buffer volumes less than 1 ml. Using previous literature related to capillary electrophoresis, a bench-scale pretreatment protocol was developed to tune specific fluidic parameters to an optimal range, namely pH, conductivity, and viscosity. A PDMS device was fabricated and used to combine a raw, bovine serum sample with specific buffer solutions. Off-chip electrodes were used to induce DC-electrokinetic micro-mixing of the target analyte in the mixing chamber, where a homogeneous analyte distribution was achieved in less than one second using an 800V DC pulse wave. Additionally, the desired solution viscosity and pH were achieved using less than 1 ml of buffer solution. Adjustment of sample conductivity, which is driven by sample fluid volume, remains an open area of research.

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