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Mechanical forces play an important role in the behavior and development of biological systems and disease at all spatial scales, from cells and their constituents to tissues and organs. Such forces have a profound influence on the health, structural integrity, and normal function of cells and organs. Accurate knowledge of cell and tissue biomechanical properties is essential to map the distribution of forces and mechanical cues in biological systems. Cell and tissue biomechanical properties are also known to be important on their own as indicators of health or disease states. Hence, optical elastography and biomechanics methods can aid in the understanding and clinical diagnosis of a wide variety of diseases. We provide a brief overview and highlight of the Optical Elastography and Tissue Biomechanics VI conference, which took place in San Francisco, February 2 and 3, 2019, as a part of Photonics West symposium.
A recently developed technique to enhance spontaneous Raman scattering utilizing a highly reflective integrating cavity is presented. Elastically scattered light circulates within the cavity volume continuously interacting with the sample, whether a bulk sample or gas, resulting in significant Raman enhancement. In addition, the Raman scattered light is collected from all directions before being coupled out of the cavity. Enhancements of 107 have been realized with the use of inexpensive low power diode lasers and a modest CCD based spectrometer. Application of the iCERS technique operating near 400 nm providing near real-time detection and measurement of trace gases, chemicals, and biological compounds is discussed.
Using Brillouin microspectroscopy to characterize adipocytes’ response to lipid droplet accumulation