Prof. William E. Moerner Profile

Prof. William E. Moerner

Harry S. Mosher Professor of Chemistry at Stanford Univ

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Publications (50)

Proceedings Article | 13 March 2024 Presentation

Optimized measurement of the 3D position and orientation of single molecules thanks to deep learning and PSF engineering

Pierre Jouchet, Anish Roy, William Moerner

Proceedings Volume PC12849, PC128490J (2024) https://doi.org/10.1117/12.3009184

KEYWORDS: Point spread functions, 3D metrology, Molecules, Engineering, Deep learning, Source mask optimization, Neural networks, Fluorescence, Signal to noise ratio, Polymers

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Proceedings Article | 5 October 2023 Presentation + Paper

Monitoring physical and chemical properties of individual carboxysomes trapped in solution

William Carpenter, Abhijit Lavania, Julia Borden, Luke Oltrogge, Davis Perez, Peter Dahlberg, David Savage, W. Moerner

Proceedings Volume 12649, 1264903 (2023) https://doi.org/10.1117/12.2679514

KEYWORDS: Fluorescence, Scattering, Particles, Interferometry, Green fluorescent proteins

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Proceedings Article | 15 March 2023 Presentation

Measuring mass and loading of single carboxysomes in the interferometric scattering anti-Brownian electrokinetic trap (Conference Presentation)

Abhijit Lavania, William Carpenter, Luke Oltrogge, Davis Perez, Julia Borden, David Savage, William Moerner

Proceedings Volume PC12386, PC1238601 (2023) https://doi.org/10.1117/12.2650912

KEYWORDS: Scattering, Luminescence, Interferometry, Particles, Light scattering, Proteins, Scatter measurement, Infrared radiation, Infrared detectors, Visible radiation

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Proceedings Article | 2 March 2022 Presentation

Trapping a blinking nanoparticle: decoupling detection and position estimation with the ISABEL trap

Allison Squires, Abhijit Lavania, Peter Dahlberg, William Moerner

Proceedings Volume PC11970, PC1197003 (2022) https://doi.org/10.1117/12.2630837

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Proceedings Article | 5 March 2021 Presentation

Exploring cell surface-nanopillar interactions with 3D super-resolution imaging

Anish Roy, Wei Zhang, Zeinab Jahed, Ching-Ting Tsai, Bianxiao Cui, W. Moerner

Proceedings Volume 11650, 1165003 (2021) https://doi.org/10.1117/12.2577319

KEYWORDS: 3D image processing, Super resolution, Proteins, Interfaces, Stereoscopy, 3D metrology, Silicon, Microscopy, Luminescence, 3D image reconstruction

Proceedings Article | 5 March 2021 Presentation

Tracking of multiple chromosomal loci in 3D throughout live mammalian cell nuclei

Anna-Karin Gustavsson, Rajarshi Ghosh, Petar Petrov, Jan Liphardt, W. Moerner

Proceedings Volume 11650, 116500J (2021) https://doi.org/10.1117/12.2578200

KEYWORDS: Point spread functions, Luminescence, Motion detection, Image resolution, 3D scanning, 3D image processing, 3D acquisition

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Proceedings Article | 13 February 2020 Presentation + Paper

Interferometric scattering for fluorescence-free electrokinetic trapping of single nanoparticles in free solution

Abhijit Lavania, Allison Squires, Peter Dahlberg, W. Moerner

Proceedings Volume 11246, 112460W (2020) https://doi.org/10.1117/12.2546638

KEYWORDS: Scattering, Particles, Luminescence, Interferometry, Nanoparticles, Light scattering, Signal detection, Laser scattering, Single molecule spectroscopy

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Proceedings Article | 13 February 2020 Presentation + Paper

Cryogenic single-molecule active control microscopy with a photoactivatable fluorescent protein

Annina Sartor, Peter Dahlberg, Jiarui Wang, Saumya Saurabh, Lucy Shapiro, W. Moerner

Proceedings Volume 11246, 112460G (2020) https://doi.org/10.1117/12.2546333

KEYWORDS: Molecules, Luminescence, Cryogenics, Proteins, Fluorescent proteins, Photons, Microscopes, Electron microscopy, Microscopy, Single molecule spectroscopy

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Proceedings Article | 20 February 2018 Presentation + Paper

Tilted light sheet microscopy with 3D point spread functions for single-molecule super-resolution imaging in mammalian cells

Anna-Karin Gustavsson, Petar Petrov, Maurice Lee, Yoav Shechtman, W. E. Moerner

Proceedings Volume 10500, 105000M (2018) https://doi.org/10.1117/12.2288443

KEYWORDS: Point spread functions, 3D image processing, Molecules, Super resolution, Microscopes, Objectives, Microscopy, Glasses, Prisms

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Proceedings Article | 24 April 2017 Presentation

Localization beyond the diffraction limit (Conference Presentation)

Petar Petrov, Yoav Shechtman, William Moerner

Proceedings Volume 10071, 1007108 (2017) https://doi.org/10.1117/12.2251307

KEYWORDS: Diffraction, Point spread functions, Microscopy, Phase retrieval, 3D image processing, Imaging systems, Computer programming, Image sensors, Sensors, 3D modeling

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Proceedings Article | 26 May 2016 Presentation

Multicolor single-molecule imaging by spectral point-spread-function engineering (Conference Presentation)

Yoav Shechtman, Lucien Weiss, Adam Backer, William Moerner

Proceedings Volume 9714, 97140L (2016) https://doi.org/10.1117/12.2208982

KEYWORDS: Point spread functions, Super resolution, Computer programming, Optical design, Dispersion, Optical components, Particles, Imaging spectroscopy, Microscopes, Single molecule spectroscopy

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Proceedings Article | 22 April 2015 Open Access

Single-molecule spectroscopy, imaging, and photocontrol: foundations for super-resolution microscopy (Presentation Video)

W. Moerner

Proceedings Volume 9331, 93311E (2015) https://doi.org/10.1117/12.2197198

KEYWORDS: Imaging spectroscopy, Chemistry, Single molecule spectroscopy, Video, Super resolution microscopy, Video microscopy, Applied physics, Physical research, Molecules, Biophysics

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Proceedings Article | 9 March 2015 Paper

Motion of chromosomal loci and the mean-squared displacement of a fractional Brownian motion in the presence of static and dynamic errors

Mikael Backlund, W. Moerner

Proceedings Volume 9331, 933106 (2015) https://doi.org/10.1117/12.2079703

KEYWORDS: Particles, Diffusion, Cameras, Motion models, Photodetectors, Error analysis, Yeast, Point spread functions, Biological research, Motion analysis

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Proceedings Article | 9 March 2015 Paper

Single-molecule exploration of photoprotective mechanisms in light-harvesting complexes

Hsiang-Yu Yang, Gabriela Schlau-Cohen, Michal Gwizdala, Tjaart Krüger, Pengqi Xu, Roberta Croce, Rienk van Grondelle, W. Moerner

Proceedings Volume 9331, 933109 (2015) https://doi.org/10.1117/12.2083628

KEYWORDS: Luminescence, Proteins, Quenching (fluorescence), Antennas, Molecular mechanisms, Light harvesting, Photosynthesis, Field programmable gate arrays, Switches, Physics

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Proceedings Article | 1 April 2014 Paper

The regulatory switch of F1-ATPase studied by single-molecule FRET in the ABEL trap

Samuel Bockenhauer, Thomas Duncan, W. Moerner, Michael Börsch

Proceedings Volume 8950, 89500H (2014) https://doi.org/10.1117/12.2042688

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Luminescence, Proteins, Switches, Photons, Confocal microscopy, Field programmable gate arrays, Fluorescence correlation spectroscopy

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Proceedings Article | 4 March 2014 Paper

Spectroscopic and transport measurements of single molecules in solution using an electrokinetic trap

Quan Wang, W. Moerner

Proceedings Volume 8950, 895004 (2014) https://doi.org/10.1117/12.2038320

KEYWORDS: Molecules, Molecular spectroscopy, Spectroscopy, Diffusion, Single molecule spectroscopy, Expectation maximization algorithms, Photons, Microfluidics, Fluorescence spectroscopy, Luminescence

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Proceedings Article | 4 March 2014 Paper

Single-molecule orientation measurements with a quadrated pupil

Adam Backer, Mikael Backlund, Matthew Lew, Alexander Diezmann, Steffen Sahl, W. Moerner

Proceedings Volume 8950, 89500L (2014) https://doi.org/10.1117/12.2042097

KEYWORDS: Molecules, Microscopes, Spatial light modulators, Objectives, Polarization, Photons, Imaging systems, Luminescence, Optical aberrations, Microscopy

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Proceedings Article | 22 February 2013 Paper

The double-helix point spread function enables precise and accurate measurement of 3D single-molecule localization and orientation

Mikael Backlund, Matthew Lew, Adam Backer, Steffen Sahl, Ginni Grover, Anurag Agrawal, Rafael Piestun, W Moerner

Proceedings Volume 8590, 85900L (2013) https://doi.org/10.1117/12.2001671

KEYWORDS: Molecules, Polarization, Microscopy, Super resolution, 3D metrology, Point spread functions, Microscopes, Luminescence, Spatial light modulators, Diffraction

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Proceedings Article | 8 May 2012 Paper

Spectrally resolved anti-Brownian electrokinetic (ABEL) trapping of single peridinin-chlorophyll-proteins in solution

Samuel Bockenhauer, Quan Wang, W. E. Moerner

Proceedings Volume 8427, 84274C (2012) https://doi.org/10.1117/12.970546

KEYWORDS: Proteins, Particles, Antennas, Photodetectors, Diffusion, Microfluidics, Luminescence, Glasses, Molecules, Field programmable gate arrays

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Proceedings Article | 13 February 2012 Paper

Anti-Brownian ELectrokinetic (ABEL) trapping of single β2-adrenergic receptors in the absence and presence of agonist

Samuel Bockenhauer, Alexandre Fuerstenberg, Xiao Jie Yao, Brian Kobilka, W. E. Moerner

Proceedings Volume 8228, 822805 (2012) https://doi.org/10.1117/12.910018

KEYWORDS: Receptors, Proteins, Photons, Neodymium, Error analysis, Ions, Glasses, Statistical analysis, Lithium, Data modeling

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Proceedings Article | 23 February 2011 Paper

Live cell single-molecule and superresolution imaging of proteins in bacteria

Julie Biteen, W. Moerner

Proceedings Volume 7905, 79050Q (2011) https://doi.org/10.1117/12.873809

KEYWORDS: Molecules, Super resolution, Proteins, Luminescence, Bacteria, Fluorescent proteins, Live cell imaging, Polymers, Image processing, Glucose

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Proceedings Article | 12 February 2011 Paper

STED super-resolution microscopy in Drosophila tissue and in mammalian cells

Lana Lau, Yin Loon Lee, Maja Matis, Jeff Axelrod, Tim Stearns, W. E. Moerner

Proceedings Volume 7910, 79101N (2011) https://doi.org/10.1117/12.881221

KEYWORDS: Stimulated emission depletion microscopy, Tissues, Proteins, Microscopy, Super resolution microscopy, Microscopes, Image resolution, Luminescence, Molecules, Super resolution

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Proceedings Article | 25 February 2010 Paper

In vivo three-dimensional superresolution fluorescence tracking using a double-helix point spread function

Matthew Lew, Michael Thompson, Majid Badieirostami, W. E. Moerner

Proceedings Volume 7571, 75710Z (2010) https://doi.org/10.1117/12.842608

KEYWORDS: Point spread functions, Quantum dots, Photons, Microscopes, Luminescence, Super resolution, Imaging systems, Microscopy, 3D image processing, Electron multiplying charge coupled devices

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Proceedings Article | 25 February 2009 Paper

Superresolution imaging in live Caulobacter crescentus cells using photoswitchable enhanced yellow fluorescent protein

Julie Biteen, Michael Thompson, Nicole Tselentis, Lucy Shapiro, W. Moerner

Proceedings Volume 7185, 71850I (2009) https://doi.org/10.1117/12.809080

KEYWORDS: Molecules, Proteins, Super resolution, Fluorescent proteins, Luminescence, Microscopy, Quantum efficiency, Polymers, Image resolution, Point spread functions

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Proceedings Article | 21 February 2009 Paper

Photoactivatable DCDHF fluorophores for single-molecule imaging

Samuel Lord, Nicholas Conley, Hsiao-lu Lee, Na Liu, Reichel Samuel, Robert Twieg, W. Moerner

Proceedings Volume 7190, 719013 (2009) https://doi.org/10.1117/12.809257

KEYWORDS: Absorption, Luminescence, Photons, Chromophores, Molecules, Quantum efficiency, Super resolution, Bioalcohols, Chemistry, Polarizability

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Proceedings Article | 29 August 2008 Paper

Hardware-based anti-Brownian electrokinetic trap (ABEL trap) for single molecules: control loop simulations and application to ATP binding stoichiometry in multi-subunit enzymes

Yan Jiang, Quan Wang, Adam Cohen, Nick Douglas, Judith Frydman, W. Moerner

Proceedings Volume 7038, 703807 (2008) https://doi.org/10.1117/12.798093

KEYWORDS: Acquisition tracking and pointing, Molecules, Particles, Photodetectors, Proteins, Microfluidics, Device simulation, Photon counting, Modulation, Luminescence

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Proceedings Article | 27 August 2005 Paper

An all-glass microfluidic cell for the ABEL trap: fabrication and modeling

Adam Cohen, W. Moerner

Proceedings Volume 5930, 59300S (2005) https://doi.org/10.1117/12.622418

KEYWORDS: Glasses, Microfluidics, Particles, Semiconducting wafers, Etching, Silicon, Molecules, Adsorption, Luminescence, Photoresist materials

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Proceedings Article | 29 March 2005 Paper

The Anti-Brownian ELectrophoretic trap (ABEL trap): fabrication and software

Adam Cohen, William Moerner

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.598689

KEYWORDS: Particles, Molecules, Cameras, Electrodes, Luminescence, Image processing, Video, Optical tweezers, Magnetism, Microscopes

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Proceedings Article | 1 July 2004 Paper

Laser background characterization in a monolithically integrated bio-fluorescence sensor

Evan Thrush, Ofer Levi, Laura Cook, Jason Deich, Stephen Smith, W. E. Moerner, James Harris

Proceedings Volume 5318, (2004) https://doi.org/10.1117/12.525133

KEYWORDS: Sensors, Photodetectors, Optical filters, Vertical cavity surface emitting lasers, Luminescence, Biological research, Interfaces, Specular reflections, Glasses, Quantum efficiency

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Proceedings Article | 14 November 2003 Paper

Novel fluorophores for single-molecule imaging

Katherine Willets, Oksana Ostroverkhova, Stephan Hess, Meng He, Robert Twieg, William Moerner

Proceedings Volume 5222, (2003) https://doi.org/10.1117/12.506519

KEYWORDS: Molecules, Luminescence, Polymers, Polymethylmethacrylate, Quantum efficiency, Photons, Absorption, Environmental sensing, Microscopes, Objectives

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Proceedings Article | 19 June 2003 Paper

Exploring novel methods of interferometric detection of ultrasmall phase shifts

Jaesuk Hwang, Martin Fejer, William Moerner

Proceedings Volume 4962, (2003) https://doi.org/10.1117/12.478913

KEYWORDS: Phase shifts, Absorption, Modulation, Laser beam diagnostics, Molecules, Polarization, Interferometers, Signal detection, Sagnac interferometers, Beam splitters

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Proceedings Article | 5 November 2002 Paper

Dicyanomethylenedihydrofuran photorefractive materials

Meng He, Robert Twieg, Oksana Ostroverkhova, Ulrich Gubler, Daniel Wright, William Moerner

Proceedings Volume 4802, (2002) https://doi.org/10.1117/12.452454

KEYWORDS: Chromophores, Glasses, Absorption, Molecules, Argon, Bromine, Carbon, Crystals, Analog electronics, Chemistry

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Proceedings Article | 5 November 2002 Paper

High-performance photorefractive organic glasses: understanding mechanisms and limitations

Oksana Ostroverkhova, Ulrich Gubler, Daniel Wright, Meng He, Robert Twieg, William Moerner

Proceedings Volume 4802, (2002) https://doi.org/10.1117/12.452413

KEYWORDS: Glasses, Chromophores, Absorption, Birefringence, Refractive index, Organic materials, Modulation, Polymers, Solids, Diffraction

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Proceedings Article | 28 March 2002 Paper

Biomolecular applications of single-molecule measurements: kinetics and dynamics of a single-enzyme reaction

Matt Paige, David Fromm, William Moerner

Proceedings Volume 4634, (2002) https://doi.org/10.1117/12.463828

KEYWORDS: Molecules, Luminescence, Proteins, Glasses, Microscopes, Confocal microscopy, Microscopy, Absorption, Green fluorescent protein, Statistical analysis

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Proceedings Article | 16 January 2002 Paper

Organic photorefractive material design strategies

Daniel Wright, Ulrich Gubler, Shaumo Sadhukhan, William Moerner, Meng He, Robert Twieg, Michael DeClue, Jay Siegel

Proceedings Volume 4462, (2002) https://doi.org/10.1117/12.452727

KEYWORDS: Composites, Polymers, Chromophores, Glasses, Absorption, Refractive index, Modulation, Molecules, Ellipsometry, Oxidation

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Proceedings Article | 13 November 2000 Paper

Photorefractive polymers for laser-based ultrasound detection

Daniel Wright, Ulrich Gubler, Marvin Klein, William Moerner

Proceedings Volume 4104, (2000) https://doi.org/10.1117/12.406474

KEYWORDS: Photorefractive polymers, Homodyne detection, Composites, Signal detection, Polymers, Ultrasonography, Beam splitters, Phase shifts, Receivers, Interferometers

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Proceedings Article | 4 February 1999 Paper

Homodyne detection of ultrasonic surface displacements using two-wave mixing in photorefractive polymers

Marvin Klein, Gerald Bacher, Anders Grunnet-Jepsen, Daniel Wright, William Moerner

Proceedings Volume 3589, (1999) https://doi.org/10.1117/12.339966

KEYWORDS: Homodyne detection, Polymers, Ultrasonics, Receivers, Two wave mixing, Interferometers, Photorefractive polymers, Signal to noise ratio, Absorption, Ultrasonography

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Proceedings Article | 23 October 1998 Paper

Trapping studies on photorefractive polymers

Daniel Wright, Anders Grunnet-Jepsen, Maria Diaz-Garcia, J. Casperson, B. Smith, Matthew Bratcher, Michael De Clue, Jay Siegel, William Moerner, Robert Twieg

Proceedings Volume 3471, (1998) https://doi.org/10.1117/12.328176

KEYWORDS: Composites, Nonlinear optics, Polymers, Absorption, Chromophores, Spectroscopy, Molecules, Solids, Photorefractive polymers, Crystals

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Proceedings Article | 24 April 1998 Paper

Probing single molecules in polyacrylamide gels

Susanne Kummer, Robert Dickson, William Moerner

Proceedings Volume 3273, (1998) https://doi.org/10.1117/12.306125

KEYWORDS: Molecules, Diffusion, Molecular spectroscopy, Polymers, Interfaces, Astatine, Liquids, Luminescence, Motion analysis, Fluorescence spectroscopy

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Proceedings Article | 13 October 1997 Paper

Recent advances in photorefractive polymer materials

William Moerner, Anders Grunnet-Jepsen, C. Thompson, Matthew Bratcher, Robert Twieg

Proceedings Volume 3147, (1997) https://doi.org/10.1117/12.284256

KEYWORDS: Polymers, Chromophores, Composites, Electro optics, Photorefractive polymers, Phase conjugation, Phase measurement, Energy transfer, Absorption, Solids

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Proceedings Article | 9 October 1997 Paper

Large-gain photorefractive polymers

Anders Grunnet-Jepsen, C. Thompson, Robert Twieg, Kevin Belfield, Matthew Bratcher, William Moerner

Proceedings Volume 3144, (1997) https://doi.org/10.1117/12.290242

KEYWORDS: Polymers, Chromophores, Composites, Electro optics, Phase conjugation, Photorefractive polymers, Phase measurement, Absorption, Solids, Light scattering

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Proceedings Article | 15 October 1996 Paper

Mechanisms of photorefractivity in polymer composites

William Moerner, Anders Grunnet-Jepsen, C. Thompson, Robert Twieg

Proceedings Volume 2850, (1996) https://doi.org/10.1117/12.254232

KEYWORDS: Polymers, Diffraction, Diffraction gratings, Modulation, Molecules, Chromophores, Nonlinear optics, Photorefractive polymers, Electro optics, Refractive index

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Proceedings Article | 23 August 1995 Paper

Photorefractivity in new organic polymeric materials

Constantina Poga, Donald Burland, Thomas Hanemann, Yan Jia, Christopher Moylan, John Stankus, Robert Twieg, William Moerner

Proceedings Volume 2526, (1995) https://doi.org/10.1117/12.217306

KEYWORDS: Polymers, Chromophores, Crystals, Diffraction, Diffraction gratings, Composites, Nonlinear optics, Holography, Holograms, Glasses

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Proceedings Article | 7 April 1995 Paper

Dynamics and vibrational spectra of individual molecules in polymer glasses

Anne Myers, Paul Tchenio, William Moerner

Proceedings Volume 2385, (1995) https://doi.org/10.1117/12.206437

KEYWORDS: Molecules, Luminescence, Raman spectroscopy, Diffusion, Chromophores, Glasses, Polymers, Molecular lasers, Crystals, Signal detection

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Proceedings Article | 23 September 1994 Paper

Recent progress in photorefractive polymers: materials and structures

John Stankus, Scott Silence, Robert Twieg, Donald Burland, Robert Miller, J. Campbell Scott, William Moerner, Gary Bjorklund

Proceedings Volume 2285, (1994) https://doi.org/10.1117/12.187516

KEYWORDS: Polymers, Diffraction, Molecules, Photorefractive polymers, Nonlinear optics, Diffraction gratings, Nondestructive evaluation, Holography, Absorption, Optical tweezers

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Proceedings Article | 16 December 1993 Paper

Lightwave transmission of multiple television signals using an organic polymer electro-optic phase modulator

Barton Smith, Mark Jurich, William Moerner, Willi Volksen, Margaret Best, William Fleming, Jerome Swalen, Gary Bjorklund

Proceedings Volume 2025, (1993) https://doi.org/10.1117/12.165289

KEYWORDS: Modulators, Electro optic polymers, Electro optics, Polymers, Signal detection, Electrodes, Televisions, Waveguides, Cladding, Heterodyning

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Proceedings Article | 25 February 1993 Paper

Nonlinear optical polymeric waveguide electro-optic phase modulator

Jerome Swalen, Gary Bjorklund, William Fleming, R. Hung, Mark Jurich, Victor Lee, Robert Miller, William Moerner, D. Morichere, Andrew Skumanich, Barton Smith

Proceedings Volume 1775, (1993) https://doi.org/10.1117/12.139215

KEYWORDS: Polymers, Waveguides, Modulators, Electro optics, Nonlinear optics, Electro optic polymers, Organic materials, Chromophores, Electrodes, Polymer thin films

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Proceedings Article | 1 December 1991 Paper

Photorefractivity in doped nonlinear organic polymers

William Moerner, C. Walsh, J. Campbell Scott, Stephen Ducharme, Donald Burland, Gary Bjorklund, Robert Twieg

Proceedings Volume 1560, (1991) https://doi.org/10.1117/12.50724

KEYWORDS: Polymers, Chromophores, Absorption, Diffraction gratings, Polarization, Electro optics, Organic materials, Diffraction, Anisotropy, Crystals

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Proceedings Article | 1 July 1991 Paper

Finding a single molecule in a haystack: laser spectroscopy of solids from (square root of)N to N=1

William Moerner, W. Patrick Ambrose

Proceedings Volume 1435, (1991) https://doi.org/10.1117/12.44247

KEYWORDS: Molecules, Molecular lasers, Absorption, Solids, Molecular spectroscopy, Luminescence, Signal detection, Spectroscopy, Ions, Frequency modulation

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Proceedings Article | 1 December 1990 Paper

Organic nonlinear optical materials and their device applications for frequency doubling, modulation, and switching

Jerome Swalen, Gary Bjorklund, Stephen Ducharme, William Fleming, Stephan Herminghaus, Dietmar Jungbauer, William Moerner, Barton Smith, Robert Twieg, Do Yoon, C. Grant Willson

Proceedings Volume 1337, (1990) https://doi.org/10.1117/12.22962

KEYWORDS: Polymers, Nonlinear optics, Crystals, Nonlinear crystals, Second-harmonic generation, Chromophores, Modulation, Organic materials, Nonlinear optical materials, Switching

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Showing 5 of 50 publications

Conference Committee Involvement (1)

Organic Photorefractive Materials II

29 July 1997 | San Diego, CA, United States

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