Multimodal imaging photoacoustic microscopy (PAM) and optical coherence tomography (OCT) can provide morphological and functional information of living organisms which reveals the physiological and pathological state of biological tissues. In this study, a dual-modal PAM and OCT system was custom-built to simultaneously image the optical absorption and scattering characteristic of rabbit eyes. Glycerol solution was used as optical clearing agent to enhance PAM and OCT imaging of New Zealand White rabbit eyes in vivo. Results showed that both OCT and PAM signals were increased by retro-bulbar injection of glycerol to New Zealand White rabbit eyes. The PAM signal amplitude increased from 0.1476 (a.u) to 0.1701 (a.u) after glycerol solution injection, which increased by 115%. And intensity of OCT signal was increased up to 150%. The optical clearing agent enhanced multimodal imaging technique provides a new method to increase the depth of optical measured depth and to improve the imaging contrast, which can be used to evaluate pathophysiological early symptoms in vivo.
The choroid is involved in the pathogenesis of several ocular conditions. However, most techniques of fundus imaging have focused on the retinal layer because the choroid is beyond the penetration depth of the detection light. In this study, a multimodal Near-infrared Photoacoustic Microscopy (NIR-PAM) and Optical Coherence Tomography (OCT) system was developed to image choroid. Polyethylene glycol (PEG) modified FI nanoparticles (FINPs) with absorption peak up to 1064 nm were used as a contrast agent to enhance NIR-PAM and OCT for in vivo imaging of choroid. After intravenous injection of PEG-FINPs into Brown Norway (BN) rats (8 weeks), the rat choroid was time-serially monitored with PAM and OCT: 1 min, 3 min, 5 min, 10 min, 15 min, 30 min, and 60 min. Signal increased by 381% in PAM and 109% in OCT at 1 min. The choroidal vessels were clearly visualized in the NIR-PAM compared with that in VIS-PAM imaging using 532 nm light source.
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