A rapid nondestructive measurement method for determining the total viable count of chilled pork was studied. Chilled
pork samples were purchased from supermarket and then stored in refrigerator at 4°C. Every 24 hours, hyperspectral
images were collected from the chilled pork samples in 400-1100nm region, in parallel total viable counts were obtained
by classical microbiological plating methods. The 3-parameter modified lorentzian distribution function was applied to
fit the scattering profiles of all samples and the fitting results were satisfactorily high in region 470-943 nm. Then the parameters extracted were used to establish PLSR models. The prediction results for the parameter a, b, c, b×c are 0.945, 0.918, 0.919, 0.935 respectively. The study show that the hyperspectral technology can accurately tracks the increase of total viable count of chilled pork during 2-14 days storage at 4°C, and so indicate it a valid tool for assessing the quality and safety properties of chilled pork rapidly and nondestructively in the future.
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