Dr. Weizhi Qi Profile

Dr. Weizhi Qi

at UESTC

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Proceedings Article | 31 October 2016 Paper

Rotary-scanning optical resolution photoacoustic microscopy

Weizhi Qi, Lei Xi

Proceedings Volume 10024, 100242T (2016) https://doi.org/10.1117/12.2245930

KEYWORDS: Imaging systems, Optical resolution, Photoacoustic microscopy, Confocal microscopy, Signal to noise ratio, Transducers, Acoustics, Photoacoustic spectroscopy, Carbon, Optical fibers

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Optical resolution photoacoustic microscopy (ORPAM) is currently one of the fastest evolving photoacoustic imaging modalities. It has a comparable spatial resolution to pure optical microscopic techniques such as epifluorescence microscopy, confocal microscopy, and two-photon microscopy, but also owns a deeper penetration depth. In this paper, we report a rotary-scanning (RS)-ORPAM that utilizes a galvanometer scanner integrated with objective to achieve rotary laser scanning. A 15 MHz cylindrically focused ultrasonic transducer is mounted onto a motorized rotation stage to follow optical scanning traces synchronously. To minimize the loss of signal to noise ratio, the acoustic focus is precisely adjusted to reach confocal with optical focus. Black tapes and carbon fibers are firstly imaged to evaluate the performance of the system, and then in vivo imaging of vasculature networks inside the ears and brains of mice is demonstrated using this system.

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