Dr. Xiangnan Zhou Profile

Dr. Xiangnan Zhou

Postdoctoral Research Associate at Univ of California Davis

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Author

Publications (21)

Proceedings Article | 13 March 2024 Presentation

Investigating fluorescence lifetime signatures for normal tissue adjacent to cancer in oropharyngeal squamous cell carcinomas

Katjana Ehrlich, Kelsey Hadfield, Lisanne Kraft, Mohamed Hassan, Brent Weyers, Xiangnan Zhou, Julien Bec, Dorina Gui, Arnaud Bewley, Marianne Abouyared, D. Gregory Farwell, Andrew Birkeland, Laura Marcu

Proceedings Volume PC12818, (2024) https://doi.org/10.1117/12.3003488

KEYWORDS: Tissues, Fluorescence, Cancer, Neck, Head, Tumors, Resection, Collagen, Windows, Time resolved spectroscopy

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Proceedings Article | 13 March 2024 Presentation

Assessing in vivo label-free fluorescence lifetime Imaging sensitivity to low-grade colon inflammation

Alba Alfonso García, Jee-Yon Lee, Alex Condon, Xiangnan Zhou, Julien Bec, Andreas Baumler, Laura Marcu

Proceedings Volume PC12822, PC1282206 (2024) https://doi.org/10.1117/12.3002008

KEYWORDS: Colon, Inflammation, In vivo imaging, Fluorescence lifetime imaging, Tissues, Mode conditioning cables, Switches, Reliability, Portability, Micro optics

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Proceedings Article | 13 March 2024 Presentation

Demonstration of a combined multispectral FLIm/polarization-sensitive OCT catheter system for biochemical/structural evaluation of atherosclerosis lesions

Julien Bec, Xiangnan Zhou, Martin Villiger, Brett Bouma, Laura Marcu

Proceedings Volume 12819, 1281908 (2024) https://doi.org/10.1117/12.3003560

KEYWORDS: Optical coherence tomography, Atherosclerosis, Imaging systems, Polarization, Imaging devices, Visualization, Tissues, Pathogens, Natural surfaces, Modulation

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Proceedings Article | 17 March 2023 Presentation

Demonstration of a combined multispectral FLIm/ polarization-sensitive OCT catheter system for biochemical/structural evaluation of atherosclerosis lesions.

Julien Bec, Xiangnan Zhou, Pelham Keahey, Martin Villiger, Brett Bouma, Laura Marcu

Proceedings Volume PC12355, PC1235505 (2023) https://doi.org/10.1117/12.2649672

KEYWORDS: Optical coherence tomography, Polarization, Multispectral imaging, Imaging systems, Imaging devices, Visualization, Tissues, Reflectivity, Pathogens, Optical fibers

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Proceedings Article | 6 March 2023 Presentation

Photon economy of pulse-sampling vs TCSPC fluorescence lifetime imaging

Xiangnan Zhou, Julien Bec, Laura Marcu

Proceedings Volume PC12370, PC1237005 (2023) https://doi.org/10.1117/12.2650311

KEYWORDS: Fluorescence lifetime imaging, Sensors, Photodetectors, Luminescence, In vivo imaging, Tissues, Surgery, Pulsed laser operation, Photonic nanostructures, Laser systems engineering

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Proceedings Article | 6 March 2023 Presentation + Paper

Intraoperative detection of IDH-mutant glioma subtype using fluorescence lifetime imaging

Silvia Noble Anbunesan, Alba Alfonso-Garcia, Xiangnan Zhou, Julien Bec, Matthew Bobinski, Han Sung Lee, Lee-way Jin, Orin Bloch, Laura Marcu

Proceedings Volume 12368, 1236803 (2023) https://doi.org/10.1117/12.2650379

KEYWORDS: Tumors, Tissues, Resection, White matter, Fluorescence, Fluorescence lifetime imaging, Surgery, Medicine, Brain tissue, Brain

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SPIE Journal Paper | 16 February 2023 Open Access

Multimodal fluorescence lifetime imaging and optical coherence tomography for longitudinal monitoring of tissue-engineered cartilage maturation in a preclinical implantation model

Xiangnan Zhou, Anne Haudenschild, Cai Li, Laura Marcu

JBO, Vol. 28, Issue 02, 026003, (February 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.2.026003

KEYWORDS: Tissues, Optical coherence tomography, Cartilage, Biological samples, In vivo imaging, Fluorescence, Quantum channels, In vitro testing, Fluorescence lifetime imaging, Nondestructive evaluation

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SignificanceCartilage tissue engineering is a promising strategy for effective curative therapies for treatment of osteoarthritis. However, tissue engineers depend predominantly on time-consuming, expensive, and destructive techniques as quality control to monitor the maturation of engineered cartilage. This practice can be impractical for large-scale biomanufacturing and prevents spatial and temporal monitoring of tissue growth, which is critical for the fabrication of clinically relevant-sized cartilage constructs. Nondestructive multimodal imaging techniques combining fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) hold great potential to address this challenge.AimThe feasibility of using multimodal FLIm–OCT for nondestructive, spatial, and temporal monitoring of self-assembled cartilage tissue maturation in a preclinical mouse model is investigated.ApproachSelf-assembled cartilage constructs were developed for 4 weeks in vitro followed by 4 weeks of in vivo maturation in nude mice. Sterile and nondestructive in situ multispectral FLIm and OCT imaging were carried out at multiple time points (t = 2, 4, and 8 weeks) during tissue development. FLIm and 3D volumetric OCT images were reconstructed and used for the analysis of tissue biochemical homogeneity, morphology, and structural integrity. A biochemical homogeneity index was computed to characterize nonhomogeneous tissue growth at different time points. OCT images were validated against histology.ResultsFLIm detects heterogenous extracellular matrix (ECM) growth of tissue-engineered cartilage. The outer edge of the tissue construct exhibited longer fluorescence lifetime in 375 to 410 and 450 to 485 nm spectral channels, indicating increase in collagen content. Significant (p < 0.05) decrease of construct homogeneity index was observed between t = 2 weeks and t = 4 weeks. Both FLIm and OCT images revealed defects (voids) at the center of the tissue construct during in vitro culture (t = 2 and 4 weeks). Cyst formation during in vivo culture was detected by OCT and confirmed with histology.ConclusionsThe ability of multimodal FLIm–OCT to nondestructively monitor the heterogenous growth of engineered tissue constructs in situ is demonstrated. Spatial and temporal variation of construct ECM component was detected by FLIm. OCT reveals structural defects (voids and cysts). This multimodal approach has great potential to replace costly destructive tests in the manufacturing of tissue-engineered medical products, facilitating their clinical translation.

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Proceedings Article | 11 January 2023 Presentation

Label-free imaging of collagen biomechanics

Benjamin Sherlock, Anne Haudenschild, Xiangnan Zhou, Clay Sheaff, J. Kent Leach, Laura Marcu, Jerry Hu, Kyriacos Athanasiou

Proceedings Volume 12333, 123330C (2023) https://doi.org/10.1117/12.2657927

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SPIE Journal Paper | 21 July 2022 Open Access

Dual-modality fluorescence lifetime imaging-optical coherence tomography intravascular catheter system with freeform catheter optics

Cai Li, Julien Bec, Xiangnan Zhou, Laura Marcu

JBO, Vol. 27, Issue 07, 076005, (July 2022) https://doi.org/10.1117/12.10.1117/1.JBO.27.7.076005

KEYWORDS: Optical coherence tomography, Luminescence, Ultraviolet radiation, Freeform optics, Infrared imaging, Imaging systems, Collimators, Tomography, Arteries, UV optics

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Proceedings Article | 9 March 2022 Presentation

Real-time interrogation of brain tumors using intraoperative FLIm

Silvia Noble Anbunesan, Alba Alfonso García, Xiangnan Zhou, Julien Bec, Roberto Frusciante, Lisanne Kraft, Brent Weyers, Matthew Bobinski, Han Sung Lee, Lee-Way Jin, Orin Bloch, Laura Marcu

Proceedings Volume PC11949, PC1194904 (2022) https://doi.org/10.1117/12.2610424

KEYWORDS: Tumors, Brain, Tissues, Neuroimaging, Luminescence, Surgery, Statistical analysis, Pulsed laser operation, Proteins, Laser tissue interaction

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Proceedings Article | 7 March 2022 Presentation

Evaluation of a high-performance FLIm/OCT catheter system for biochemical and structural evaluation of atherosclerosis

Julien Bec, Cai Li, Xiangnan Zhou, Deborah Vela, Laura Marcu

Proceedings Volume PC11936, PC1193604 (2022) https://doi.org/10.1117/12.2607844

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Proceedings Article | 7 March 2022 Presentation

Real-time intraoperative fluorescence lifetime imaging of 5-ALA induced PpIX and autofluorescence in brain tumors: first results in patients

Xiangnan Zhou, Silvia Anbunesan, Alba Alfonso García, Julien Bec, Lisanne Kraft, Roberto Frusciante, Orin Bloch, Laura Marcu

Proceedings Volume PC11943, PC1194305 (2022) https://doi.org/10.1117/12.2612597

KEYWORDS: Tumors, Brain, Luminescence, Fluorescence lifetime imaging, Neuroimaging, Visualization, Tissues, Surgery, Video, System identification

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Proceedings Article | 5 March 2022 Presentation + Paper

Optimizing the light penetration depth in APDs and SPADs for high gain-bandwidth and ultra-wide spectral response

Ahasan Ahamed, Cesar Bartolo-Perez, Ahmed Sulaiman Mayet, Soroush Ghandiparsi, Gerard Ariño-Estrada, Xiangnan Zhou, Julien Bec, Shih-Yuan Wang, Laura Marcu, M. Saif Islam

Proceedings Volume 12009, 1200905 (2022) https://doi.org/10.1117/12.2610526

KEYWORDS: Avalanche photodetectors, Nanostructures, Absorption, Silicon, Photonic nanostructures, Sensors, Nanostructuring, Fluorescence lifetime imaging, Modulation, Avalanche photodiodes

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SPIE Journal Paper | 2 February 2022 Open Access

First in patient assessment of brain tumor infiltrative margins using simultaneous time-resolved measurements of 5-ALA-induced PpIX fluorescence and tissue autofluorescence

Alba Alfonso García, Xiangnan Zhou, Julien Bec, Silvia Anbunesan, Farzad Fereidouni, Lee-Way Jin, Han Sung Lee, Orin Bloch, Laura Marcu

JBO, Vol. 27, Issue 02, 020501, (February 2022) https://doi.org/10.1117/12.10.1117/1.JBO.27.2.020501

KEYWORDS: Luminescence, Tumors, Tissues, Brain, Microscopes, In vivo imaging, Surgery, Video, Data acquisition, Visualization

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Significance: 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PpIX) fluorescence is currently used for image-guided glioma resection. Typically, this widefield imaging method highlights the bulk of high-grade gliomas, but it underperforms at the infiltrating edge where PpIX fluorescence is not visible to the eyes. Fluorescence lifetime imaging (FLIm) has the potential to detect PpIX fluorescence below the visible detection threshold. Moreover, simultaneous acquisition of time-resolved nicotinamide adenine (phosphate) dinucleotide [NAD(P)H] fluorescence may provide metabolic information from the tumor environment to further improve overall tumor detection. Aim: We investigate the ability of pulse sampling, fiber-based FLIm to simultaneously image PpIX and NAD(P)H fluorescence of glioma infiltrative margins in patients. Approach: A mesoscopic fiber-based point-scanning FLIm device (355 nm pulses) was used to simultaneously resolve the fluorescence decay of PpIX (629/53 nm) and NAD(P)H (470/28 nm). The FLIm device enabled data acquisition at room light and rapid (<33 ms) augmentation of FLIm parameters on the surgical field-of-view. FLIm measurements from superficial tumors and tissue areas around the resection margins were performed on three glioblastoma patients in vivo following inspection of PpIX visible fluorescence with a conventional neurosurgical microscope. Microbiopsies were collected from FLIm imaged areas for histopathological evaluation. Results: The average lifetime from PpIX and NAD(P)H fluorescence distinguished between tumor and surrounding tissue. FLIm measurements of resection margins presented a range of PpIX and NAD(P)H lifetime values (τPpIX   ∼ 3 to 14 ns, τNAD(P)H = 3 to 6 ns) associated with unaffected tissue and areas of low-density tumor infiltration. Conclusions: Intraoperative FLIm could simultaneously detect the emission of PpIX and NAD(P)H from patients in vivo during craniotomy procedures. This approach doubles as a clinical tool to identify tumor areas while performing tissue resection and as a research tool to study tumor microenvironmental changes in vivo. Intraoperative FLIm of 5-ALA-induced PpIX and tissue autofluorescence makes a promising surgical adjunct to guide tumor resection surgery.

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Proceedings Article | 3 August 2021 Paper

Controlling light penetration depth to amplify the gain in ultra-fast silicon APDs and SPADs using photon-trapping nanostructures

Ahasan Ahamed, Cesar Bartolo-Perez, Ahmed Sulaiman Mayet, Soroush GhandiParsi, Xiangnan Zhou, Julien Bec, Nibir K. Dhar, Ekaterina P. Devine, Shih-Yuan Wang, Gerard Ariño-Estrada, Laura Marcu, M. Saif Islam

Proceedings Volume 11800, 118000F (2021) https://doi.org/10.1117/12.2597835

KEYWORDS: Absorption, Silicon, Avalanche photodetectors, Photonic nanostructures, Nanostructures, Avalanche photodiodes, Etching, Photonic devices, Photodiodes, Sensors

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Proceedings Article | 5 March 2021 Presentation

Multispectral fluorescence lifetime imaging system with Si avalanche photodetector: a better platform for clinical application

Xiangnan Zhou, Julien Bec, Diego Yankelevich, Laura Marcu

Proceedings Volume 11631, 116310E (2021) https://doi.org/10.1117/12.2579121

KEYWORDS: Imaging systems, Multispectral imaging, Fluorescence lifetime imaging, Avalanche photodetectors, Silicon, Tissues, Luminescence, Ultraviolet radiation, Tumors

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Proceedings Article | 5 March 2021 Presentation

High performance FLIm/OCT catheter system compatible for biochemical/structural evaluation of atherosclerosis

Cai Li, Julien Bec, Xiangnan Zhou, Laura Marcu

Proceedings Volume 11621, 116210P (2021) https://doi.org/10.1117/12.2577322

KEYWORDS: Optical coherence tomography, Solid state electronics, Integrated optics, Collimators, Tissues, Spectroscopy, Sensors, Microchannel plates, Light emitting diodes

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Proceedings Article | 9 March 2020 Presentation

FLIm guided Raman imaging for detecting bovine pericardium cross-links and calcification (Conference Presentation)

Tanveer Ahmed Shaik, Alba Alfonso Garcia, Xiangnan Zhou, Anne Haudenschild, Christoph Krafft, Laura Marcu, Jürgen Popp

Proceedings Volume 11215, 112150I (2020) https://doi.org/10.1117/12.2545632

KEYWORDS: Raman spectroscopy, Fluorescence lifetime imaging, Analog electronics, Target acquisition, Data acquisition, Luminescence, Tissues, Nondestructive evaluation

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Proceedings Article | 7 March 2019 Presentation

Fluorescence lifetime imaging and intravascular ultrasound (FLIm/IVUS) platform for label-free intraluminal characterization of vascular biomaterials in vitro and in vivo (Conference Presentation)

Alba Alfonso García, Anne Haudenschild, James Mcmasters, Julien Bec, Xiangnan Zhou, Alyssa Panitch, Leigh Griffiths, Laura Marcu

Proceedings Volume 10855, 1085509 (2019) https://doi.org/10.1117/12.2510816

KEYWORDS: In vivo imaging, In vitro testing, Fluorescence lifetime imaging, Arteries, Tissues, Intravascular ultrasound, Structured optical fibers, Multispectral imaging, Imaging systems, Natural surfaces

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Proceedings Article | 4 March 2019 Presentation

Multimodal evaluation of tissue engineered cartilage maturation in a pre-clinical implantation model (Conference Presentation)

Anne Haudenschild, Xiangnan Zhou, Cai Li, Jerry Hu, J. Kent Leach, Kyriacos Athanasiou, Laura Marcu

Proceedings Volume 10876, 108760A (2019) https://doi.org/10.1117/12.2509047

KEYWORDS: Tissues, Cartilage, Optical coherence tomography, Nondestructive evaluation, Tissue optics, Optics manufacturing, Imaging systems, Fluorescence lifetime imaging, Fiber optics, Interfaces

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SPIE Journal Paper | 21 December 2018 Open Access

Detection of glycosaminoglycan loss in articular cartilage by fluorescence lifetime imaging

Xiangnan Zhou, Anne Haudenschild, Benjamin Sherlock, Jerry Hu, J. Kent Leach, Kyriacos Athanasiou, Laura Marcu

JBO, Vol. 23, Issue 12, 126002, (December 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.12.126002

KEYWORDS: Cartilage, Luminescence, Collagen, Fluorescence lifetime imaging, Tissues, Image segmentation, Statistical analysis, Imaging spectroscopy, In vivo imaging, Signal detection

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