Mr. Yash Mantri Profile

Yash Mantri

at Univ of California San Diego

SPIE Involvement:

Author

Publications (4)

Proceedings Article | 6 March 2023 Presentation

Impact of skin tone on photoacoustic signal generation, depth penetration, and oximetry

Jesse Jokerst, Yash Mantri, Wonjun Yim

Proceedings Volume PC12370, PC123700E (2023) https://doi.org/10.1117/12.2657721

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The major optical absorbers in tissue are melanin and oxy/deoxy-hemoglobin, but the impact of skin tone and pigmentation on biomedical optics is still not completely understood or adequately addressed. Melanin largely governs skin tone with higher melanin concentration in subjects with darker skin tones. Recently, there has been extensive debate on the bias of pulse oximeters when used with darker subjects. Photoacoustic (PA) imaging can measure oxygen saturation similarly as pulse oximeters and could have value in studying this bias. More importantly, it can deconvolute the signal from the skin and underlying tissue. Here, we studied the impact of skin tone on PA signal generation, depth penetration, and oximetry. Our results show that subjects with darker skin tones exhibit significantly higher PA signal at the skin surface, reduced penetration depth, and lower oxygen saturation compared to subjects with lighter skin tones. We then suggest a simple way to compensate for these signal differences. More recently, we have developed 3D-bioprinted skin-mimicking phantoms with skin colors ranging across the Fitzpatrick scale. These tools can help understand the impact of skin phototypes on biomedical optics. Synthetic melanin nanoparticles of different sizes (70–500 nm) and clusters were fabricated to mimic the optical behavior of melanosome. The absorption coefficient and reduced scattering coefficient of the phantoms are comparable to real human skin. We further validated the melanin content and distribution in the phantoms versus real human skins via photoacoustic (PA) imaging. The PA signal of the phantom could be improved by (i) increasing melanin size (>1,000-fold), (ii) increasing clustering (2–10.5-fold), and (iii) increasing concentration (1.3–8-fold). We then used multiple biomedical optics tools (e.g., PA, fluorescence imaging and photothermal therapy) to understand the impact of skin tone on these modalities. These well-defined 3D-bioprinted phantoms may have value in translating biomedical optics and reducing racial bias.