Prof. Young-Pil Kim Profile

Prof. Young-Pil Kim

at Hanyang Univ

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Proceedings Article | 8 July 2015 Paper

Analysis of antifreeze protein activity using colorimetric gold nanosensors

Xu Jing, Ho-seok Choi, Ji-In Park, Young-Pil Kim

Proceedings Volume 9523, 95230C (2015) https://doi.org/10.1117/12.2189039

KEYWORDS: Proteins, Gold, Crystals, Nanosensors, Nanoparticles, Biotechnology, Molecular self-assembly, Imaging spectroscopy, Organisms, Spectroscopy

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High activity and long stability of antifreeze proteins (AFPs), also known as ice-binding proteins (IBPs), are necessary for exerting their physiological functions in biotechnology and cryomedicine. Here we report a simple analysis of antifreeze protein activity and stability based on self-assembly of gold nanoparticles (AuNPs) via freezing and thawing cycles. While the mercaptosuccinic acid-capped AuNP (MSA-AuNP) was easily self-assembled after a freezing/thawing cycle, due to the mechanical attack of ice crystal on the MSA-AuNP surface, the presence of AFP impeded the self-assembly of MSA-AuNP via the interaction of AFP with ice crystals via freezing and thawing cycles, which led to a strong color in the MSA-AuNP solution. As a result, the aggregation parameter (E₅₂₀/E₆₅₀) of MSA-AuNP showed the rapid detection of both activity and stability of AFPs. We suggest that our newly developed method is very suitable for measuring antifreeze activity and stability in a simple and rapid manner with reliable quantification.

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