Prof. Yusuke Inoue Profile

Prof. Yusuke Inoue

at Kagawa Univ

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Publications (2)

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Proceedings Article | 6 December 2005 Paper

Variable phase-contrast spectrometry for reconstructing the 3-dimensional distribution of components in single living cells

Y. Inoue, I. Ishimaru, T. Yasokawa, K. Ishizaki, M. Yoshida

Proceedings Volume 6049, 60490A (2005) https://doi.org/10.1117/12.648324

KEYWORDS: Spectroscopy, Refractive index, Absorption, Optical filters, Microscopes, Spatial resolution, Phase shifts, Mirrors, Cancer, Image processing

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We propose the spectroscopy-tomography of single cells to improve the early detection and treatment of cancer. This technology can obtain the 3-dimensional distribution of components at a high spatial resolution. In this paper, we mention the analysis result of the cross-sectional images of the microsphere whose diameter is 10 μm. The distribution of the internal submicron-defect in the microsphere can be analyzed. To obtain the correct 3-dimensional absorption distribution, the axial runout can not be allowed. However, the center of rotation is displaced because the cells have complex refractive index distribution. Therefore we propose the image processing that uses the normalized correlation function as estimated value. The cross-sectional image of the microsphere is improved and the vague internal defect becomes to be distinguished by this proposed method. Moreover, based on this method, the 3-dimentional refractive index distribution in a single cell is estimated and the part which has a high refractive index in the cell is distinguished clearly. And we mention the proposed variable phase-contrast spectrometry as the 2-dimensional high spatial resolution spectrometry. This proposed method is a phase-shift interferometry between the 0th order diffracted light and the higher order diffracted light. We discuss the experimental results of the spectral characteristics using the proposed variable phase-contrast spectrometry. We measured the spectral characteristics at each pixel using the color filter of the liquid crystal and verified that the 2-dimensional spectral characteristics can be measured with good result.

Proceedings Article | 5 December 2005 Paper

6-DOF control of single living cells by proximal two-beam optical tweezers

M. Yoshida, I. Ishimaru, K. Ishizaki, Y. Inoue, T. Yasokawa

Proceedings Volume 6048, 604804 (2005) https://doi.org/10.1117/12.648342

KEYWORDS: Optical tweezers, Velocity measurements, Spatial filters, Mirrors, Absorption, Refractive index, Spatial frequencies, Spectroscopy, Diodes, Fourier transforms

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We performed a spectroscopy-tomography study of a single living cell to obtain 3-dimensional distribution of proteins in high spatial resolution in real time. In this report, we mention the 6-DOF manipulation of a single living cell to achieve the high spatial resolution 3-dimentional spectrometry. We propose the proximal two- beam optical tweezers as rotational operation. We decided to illuminate the proximal two points in each from different directions using two beams. In this case, the light pressure generated by light absorption is made to act as rotating torque. Using this proposed method, we can operate the rotational velocity of a microsphere regardless of refractive index distribution by non-contact operation. In addition, rotational speed is controlled by optical PWM operation. This proposed optical PWM operation is that the received light intensity is changed by the illumination time. This method can be developed into the 6-DOF control of single-cell. And we propose the optical spatial filtering method, paying attention to the diffracted light that is generated from a sample, as translational velocity measurement. This measurement derives the arbitrary component of the spatial frequency from the random refracted index distribution as the periodic light intensity distribution. This periodic light intensity distribution changes in accordance with the translation of an object. Therefore, we can obtain the translational velocity of the non- labeled cell by high-response photodiode.

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