Prof. Giuseppe Giberto Chirico Profile

Prof. Giuseppe Giberto Chirico

at Univ degli Studi di Milano-Bicocca

SPIE Involvement:

Author

Publications (33)

Proceedings Article | 13 March 2024 Presentation

Presentation

Rapid fabrication of a bioimaging micro-chip using two-photon polymerization

Behjat Kariman, Alessandra Nardini, Marco Grassi, Mario Marini, Claudio Conci, Margaux Bouzin, Maddalena Collini, Manuela Raimondi, Giuseppe Chirico, Roberto Osellame, Giulio Cerullo, Rebeca Martínez Vázquez

Proceedings Volume PC12875, PC1287503 (2024) https://doi.org/10.1117/12.3002218

KEYWORDS: Two photon polymerization, Fabrication, Biological imaging, Microlens, In vivo imaging, Windows, Lenses, Confocal microscopy, Voxels, Ultraviolet radiation

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Proceedings Article | 12 March 2024 Presentation + Paper

Presentation + Paper

Non-linear excitation microscopy of live cells by using an implantable microscope objective-on-a-chip

A. Nardini, R. Martínez Vázquez, C. Conci, M. Grassi, M. Marini, M. Bouzin, M. Collini, R. Osellame, G. Cerullo, B. Kariman, M. Raimondi, G. Chirico

Proceedings Volume 12846, 1284607 (2024) https://doi.org/10.1117/12.3001604

KEYWORDS: Microlens, Objectives, Ultraviolet radiation, Two photon polymerization, Microscopes, Fabrication, Scanning electron microscopy, Biological imaging, Microfabrication, Fluorescence

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Proceedings Article | 12 August 2023 Presentation

Presentation

Virtually stained H&E images and nuclei segmentation combining neural networks and spectral phasor analysis

Elena Pagani, Davide Panzeri, Riccardo Scodellaro, Margaux Bouzin, Laura D'Alfonso, Maddalena Collini, Giuseppe Chirico, Donato Inverso, Laura Sironi

Proceedings Volume PC12622, PC126220J (2023) https://doi.org/10.1117/12.2673774

KEYWORDS: Image segmentation, Biological research, Neural networks, Nervous system, Microscopes, Tumors, Tissues, Pathology, Liver, Image analysis

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Proceedings Article | 12 August 2023 Presentation

Presentation

SuperμMAPPS: a supervised AI-phasor-based method to investigate collagen micro-architecture and early detect tumorous tissue in human samples

Riccardo Scodellaro, Davide Panzeri, Elena Pagani, Margaux Bouzin, Laura D'Alfonso, Maddalena Collini, Giuseppe Chirico, Laura Sironi

Proceedings Volume PC12622, PC126220A (2023) https://doi.org/10.1117/12.2673863

KEYWORDS: Tumors, Tissues, Collagen, Biological samples, Cancer detection, Second harmonic generation, Statistical analysis, Signal generators, Visualization, Thyroid

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Proceedings Article | 12 August 2023 Presentation

Presentation

Fibrosis detection and quantification in whole slide images through deep learning

Davide Panzeri, Elena Pagani, Riccardo Scodellaro, Giuseppe Chirico, Luca Di Tommaso, Donato Inverso, Laura Sironi

Proceedings Volume PC12622, PC126220K (2023) https://doi.org/10.1117/12.2673779

KEYWORDS: Image segmentation, Deep learning, Collagen, RGB color model, Liver, Education and training, Visualization, Pathology, Nervous system, Matrices

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Proceedings Article | 11 August 2023 Presentation + Paper

Presentation + Paper

Two-photon laser polymerized microlenses for non-linear excitation microscopy of biological samples

A. Nardini, R. Martínez Vázquez, M. Marini, C. Conci, M. Bouzin, M. Collini, R. Osellame, G. Cerullo, B. Kariman, M. Farsari, E. Kabouraki, G. Chirico, M. Raimondi

Proceedings Volume 12622, 1262206 (2023) https://doi.org/10.1117/12.2672894

KEYWORDS: Microlens, Two photon polymerization, Nonlinear optics, Fabrication, Biological samples, Polymers, Biological imaging, Polymerization, In vivo imaging, Microscopy

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Proceedings Article | 27 May 2022 Paper

Paper

A miniaturized chip for 3D optical imaging of tissue regeneration in vivo

Claudio Conci, Emanuela Jacchetti, Laura Sironi, Lorenzo Gentili, Giulio Cerullo, Rebeca Martinez, Roberto Osellame, Mario Marini, Margaux Bouzin, Maddalena Collini, Laura D'Alfonso, Elmina Kabouraki, Maria Farsari, Anthi Ranella, Nikos Kehagias, Giuseppe Chirico, Manuela Raimondi

Proceedings Volume 12144, 121440D (2022) https://doi.org/10.1117/12.2629824

KEYWORDS: Tissues, Microscopy, In vivo imaging, Confocal microscopy, Two photon polymerization, Tissue optics, Spatial light modulators, Multiphoton fluorescence microscopy

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Proceedings Article | 24 June 2021 Presentation

Presentation

IN2SIGHT: an in vivo bioengineered chip for new validation protocols of biomaterials

Giuseppe Chirico

Proceedings Volume 11786, 1178608 (2021) https://doi.org/10.1117/12.2595057

KEYWORDS: In vivo imaging, Optical imaging, Two photon polymerization, Polymerization, Laser tissue interaction, Deep tissue imaging

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Proceedings Article | 20 June 2021 Presentation + Paper

Presentation + Paper

Micro structured tools for cell modeling in the fourth dimension

Manuela Raimondi, Bianca Barzaghini, Alberto Bocconi, Claudio Conci, Chiara Martinelli, Alessandra Nardini, Carolina Testa, Stephana Carelli, Giulio Cerullo, Giberto Chirico, Riccardo Gottardi, Roberto Osellame, Andrea Remuzzi, Matteo Laganà, Emanuela Jacchetti

Proceedings Volume 11786, 117861T (2021) https://doi.org/10.1117/12.2593332

KEYWORDS: Stem cells, Laser applications, Visualization, Therapeutic agents, Polymers, Polymerization, Nanotechnology, Nanofabrication, Modeling, Microscopes

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Proceedings Article | 20 June 2021 Presentation

Presentation

Development and characterization of proteinaceous multiphoton-fabricated microstructures with photo-thermal functionality

Mario Marini, Amirbahador Zeynali, Margaux Bouzin, Laura Sironi, Laura D'Alfonso, Piersandro Pallavicini, Maddalena Collini, Giuseppe Chirico

Proceedings Volume 11786, 117861U (2021) https://doi.org/10.1117/12.2593350

KEYWORDS: Nanoparticles, Microfluidics, 3D microstructuring, Tunable lasers, Thermography, Spatial resolution, Proteins, Printing, Neurons, Multiphoton lithography

Read Abstract +

We exploit two-photon laser writing to fabricate 3D biocompatible proteinaceous microstructures (∼1 to 50 𝜇m in lateral size) with tunable elasticity and photo-thermal activity in the near-infrared. Structure printing relies on the photo-crosslinking of the protein bovine serum albumin (BSA, 50 mg/mL) initiated by the Rose Bengal dye (2 mM concentration), whereas photo-thermal functionality is achieved by the dispersion of non-spherically symmetric metallic nanoparticles into the ink. Aiming at a subsequent application of the fabricated microstructures as platforms for cell growth and stimulation, we carry out a thorough characterization of their mechanical and photo-thermal properties. Preliminary data obtained by AFM indentation have quantified the structures Young modulus in the broad 100-1000 kPa range depending on the BSA concentration. Stiffness is further characterized here by subjecting the fabricated microstructures to steady flow in a microfluidic device, and by quantifying their real-time bending by a conventional transmitted light microscope. In parallel, we focus on the optimization of the photo-thermal activity of the structures. Anisotropic gold nanoparticles, dispersed in the ink, get trapped into the structure during photo-crosslinking and lead to localized heat release upon excitation in the near-infrared. The temperature increment is rapidly (∼1 s) reached and maintained under continuous wave laser irradiation at 800 nm; the amplitude of the temperature variation is quantified as a function of the incident laser power by means of infrared thermography and is correlated to both the structure thickness and the nanoparticles concentration. The resulting spatially confined heat loads could be exploited to induce highly localized responses in cells. In this direction, proteinaceous photo-thermal microstructures can be used to physically induce the differentiation of cells (e.g. neurons or fibroblasts) in a spatially controlled manner.

SPIE Journal Paper | 27 February 2019 Open Access

Open Access

Adaptive optics microspectrometer for cross-correlation measurement of microfluidic flows

Maddalena Collini, Fabrizio Radaelli, Laura Sironi, Nicolo Ceffa, Laura D'Alfonso, Margaux Bouzin, Giuseppe Chirico

JBO, Vol. 24, Issue 02, 025004, (February 2019) https://doi.org/10.1117/12.10.1117/1.JBO.24.2.025004

KEYWORDS: Spatial light modulators, Aberration correction, Adaptive optics, Cameras, Optical aberrations, Wavefronts, Microfluidics, Statistical analysis, Luminescence, Rhodamine

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SPIE Journal Paper | 13 October 2017 Open Access

Open Access

Spatiotemporal image correlation analysis of blood flow in branched vessel networks of zebrafish embryos

Nicolo Ceffa, Ilaria Cesana, Maddalena Collini, Laura D’Alfonso, Silvia Carra, Franco Cotelli, Laura Sironi, Giuseppe Chirico

JBO, Vol. 22, Issue 10, 106008, (October 2017) https://doi.org/10.1117/12.10.1117/1.JBO.22.10.106008

KEYWORDS: Image analysis, Blood circulation, Blood, Imaging spectroscopy, Capillaries, In vivo imaging, Spatial resolution, Spectroscopy, Objectives, Microfluidics

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Proceedings Article | 26 June 2017 Paper

Paper

Scanless nonlinear optical microscope for image reconstruction and space-time correlation analysis

N. Ceffa, F. Radaelli, P. Pozzi, M. Collini, L. Sironi, L. D'alfonso, G. Chirico

Proceedings Volume 10333, 103330E (2017) https://doi.org/10.1117/12.2271832

KEYWORDS: Spatial light modulators, Microscopes, Charge-coupled devices, Image analysis, Image acquisition, Tissues, Blood circulation, Tissue optics, Nonlinear optics, Microscopy, Luminescence, Optical microscopes, Image restoration, Biological research, Modulators, Fluorescence spectroscopy, Spectroscopy

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Proceedings Article | 27 April 2016 Paper

Paper

Photothermal effect of gold nanostars inkjet-printed on coated paper substrate under near-infrared irradiation

Mykola Borzenkov, Giuseppe Chirico, Maddalena Collini, Anni Määttänen, Petri Ihalainen, Elisa Cabrini, Giacomo Dacarro, Piersandro Pallavicini

Proceedings Volume 9887, 98872C (2016) https://doi.org/10.1117/12.2229824

KEYWORDS: Near infrared, Gold, Photothermal effect, Biomedical optics, Therapeutics, Inkjet technology, Printing, Medical research, Nanoparticles, Polymers, Photothermal effect, Coating, Temperature metrology

Read Abstract +

Proceedings Article | 27 April 2016 Paper

Paper

Gold nanocages for imaging and therapy of prostate cancer cells

Laura Sironi, Svetlana Avvakumova, Elisabetta Galbiati, Silvia Locarno, Chiara Macchi, Laura D'Alfonso, Massimiliano Ruscica, Paolo Magni, Maddalena Collini, Sergio Romeo, Giuseppe Chirico, Davide Prosperi

Proceedings Volume 9887, 988721 (2016) https://doi.org/10.1117/12.2229715

KEYWORDS: Prostate cancer, Gold, Biomedical optics, Confocal microscopy, Cancer, Prostate cancer, Cancer, Luminescence, Nanoparticles, Near infrared, Pulsed laser operation, Tissues, Microscopy

Read Abstract +

Proceedings Article | 22 June 2015 Paper

Paper

Single image correlation for blood flow mapping in complex vessel networks

Giuseppe Chirico, Laura Sironi, Margaux Bouzin, Laura D'Alfonso, Maddalena Collini, Nicolo’ Ceffa, Cassia Marquezin

Proceedings Volume 9529, 95290F (2015) https://doi.org/10.1117/12.2190739

KEYWORDS: Blood circulation, Confocal microscopy, Luminescence, Diffusion, Capillaries, Image analysis, Hemodynamics, Tissues, Spatial resolution, Fluorescence correlation spectroscopy

Read Abstract +

Microcirculation plays a key role in the maintenance and hemodynamics of tissues and organs also due to its extensive interaction with the immune system. A critical limitation of state-of-the-art clinical techniques to characterize the blood flow is their lack of the spatial resolution required to scale down to individual capillaries. On the other hand the study of the blood flow through auto- or cross-correlation methods fail to correlate the flow speed values with the morphological details required to describe an intricate network of capillaries. Here we propose to use a newly developed technique (FLICS, FLow Image Correlation Spectroscopy) that, by employing a single raster-scanned xy-image acquired in vivo by confocal or multi-photon excitation fluorescence microscopy, allows the quantitative measurement of the blood flow velocity in the whole vessel pattern within the field of view, while simultaneously maintaining the morphological information on the immobile structures of the explored circulatory system. Fluorescent flowing objects produce diagonal lines in the raster-scanned image superimposed to static morphological details. The flow velocity is obtained by computing the Cross Correlation Function (CCF) of the intensity fluctuations detected in pairs of columns of the image. The whole analytical dependence of the CCFs on the flow speed amplitude and the flow direction has been reported recently. We report here the derivation of approximated analytical relations that allows to use the CCF peak lag time and the corresponding CCF value, to directly estimate the flow speed amplitude and the flow direction. The validation has been performed on Zebrafish embryos for which the flow direction was changed systematically by rotating the embryos on the microscope stage. The results indicate that also from the CCF peak lag time it is possible to recover the flow speed amplitude within 13% of uncertainty (overestimation) in a wide range of angles between the flow and the image scanning direction.

Proceedings Article | 5 March 2015 Paper

Paper

Fluorescence cross-correlation spectroscopy for time dependent flows: a numerical investigation

Nicolo' Ceffa, Paolo Pozzi, Margaux Bouzin, Cassia Marquezin, Laura Sironi, Laura D'Alfonso, Maddalena Collini, Giuseppe Chirico

Proceedings Volume 9320, 93200R (2015) https://doi.org/10.1117/12.2077088

KEYWORDS: Modulation, Capillaries, Luminescence, Diffusion, Blood, Blood circulation, Microfluidics, Fluorescence spectroscopy, Spectroscopy, Numerical analysis

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SPIE Journal Paper | 9 February 2015 Open Access

Open Access

High-throughput spatial light modulation two-photon microscopy for fast functional imaging

Paolo Pozzi, Daniela Gandolfi, Marialuisa Tognolina, Giuseppe Chirico, Jonathan Mapelli, Egidio D’Angelo

NPh, Vol. 2, Issue 01, 015005, (February 2015) https://doi.org/10.1117/12.10.1117/1.NPh.2.1.015005

KEYWORDS: Spatial light modulators, Calcium, Luminescence, Signal detection, Sensors, Confocal microscopy, Two photon excitation microscopy, Microscopes, Modulation, Diffraction

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SPIE Journal Paper | 20 June 2014 Open Access

Open Access

Electron multiplying charge-coupled device-based fluorescence cross-correlation spectroscopy for blood velocimetry on zebrafish embryos

Paolo Pozzi, Laura Sironi, Laura D’Alfonso, Margaux Bouzin, Maddalena Collini, Giuseppe Chirico, Piersandro Pallavicini, Franco Cotelli, Efrem Foglia

JBO, Vol. 19, Issue 06, 067007, (June 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.6.067007

KEYWORDS: Diffusion, Capillaries, Luminescence, Blood, Veins, Blood circulation, Solids, Fluorescence spectroscopy, Spectroscopes, Arteries

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Proceedings Article | 1 March 2013 Paper

Paper

Structured illumination fluorescence correlation spectroscopy for velocimetry in Zebrafish embryos

Paolo Pozzi, Leone Rossetti, Laura Sironi, Stefano Freddi, Laura D'Alfonso, Michele Caccia, Margaux Bouzin, Maddalena Collini, Giuseppe Chirico

Proceedings Volume 8580, 85800V (2013) https://doi.org/10.1117/12.2002082

KEYWORDS: Diffusion, Luminescence, Blood, Veins, Fluorescence correlation spectroscopy, Gold, Solids, Rhodamine, Signal detection, Nanorods

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Proceedings Article | 13 February 2010 Paper

Paper

In-vitro and in-vivo detection of p53 by fluorescence lifetime on a hybrid FITC-gold nanosensor

L. Sironi, S. Freddi, L. D'Alfonso, M. Collini, T. Gorletta, S. Soddu, G. Chirico

Proceedings Volume 7574, 757403 (2010) https://doi.org/10.1117/12.841094

KEYWORDS: Gold, Proteins, Luminescence, In vitro testing, Technetium, In vivo imaging, Tumors, Nanoparticles, Nanosensors, Cancer

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Proceedings Article | 12 February 2010 Paper

Paper

Two photon microscopy intravital study of DC-mediated anti-tumor response of NK cells

Michele Caccia, Tatiana Gorletta, Laura Sironi, Ivan Zanoni, Cristina Salvetti, Maddalena Collini, Francesca Granucci, Giuseppe Chirico

Proceedings Volume 7565, 75650Q (2010) https://doi.org/10.1117/12.842214

KEYWORDS: Diffusion, Tissues, Two photon excitation microscopy, Pathogens, Microscopes, In vivo imaging, Signal detection, Physics, Microscopy, Inspection

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Proceedings Article | 21 June 2004 Paper

Paper

Scanning algorithms in high-sensitivity two-photon excitation microscopy for single-molecule investigations

Giuseppe Chirico, Gabriele Malengo, Roberto Milani, Fabio Cannone, Silke Krol, Alberto Diaspro

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.537915

KEYWORDS: Luminescence, Molecules, Imaging spectroscopy, Avalanche photodetectors, Spectroscopy, Analog electronics, Photon counting, Confocal microscopy, Glasses, Raster graphics

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Proceedings Article | 9 October 2003 Paper

Paper

Novel efficient and stable heteroaromatic two-photon absorbing dyes

Alessandro Abbotto, Luca Beverina, Giorgio Pagani, Maddalena Collini, Giuseppe Chirico, Laura D'Alfonso, Giancarlo Baldini

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500564

KEYWORDS: Absorption, Chromophores, Nitrogen, Luminescence, Molecules, Near infrared, Optical properties, Biomedical optics, Visible radiation, Quantum efficiency

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Proceedings Article | 9 October 2003 Paper

Paper

New two-photon excitation chromophores for cellular imaging

Laura D'Alfonso, Giuseppe Chirico, Maddalena Collini, Giancarlo Baldini, Alberto Diaspro, Paola Ramoino, Alessandro Abbotto, Luca Beverina, Giorgio Pagani

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500637

KEYWORDS: Luminescence, Fluorescence correlation spectroscopy, Two photon excitation microscopy, Bioalcohols, Confocal microscopy, Absorption, Rhodamine, Yeast, Proteins, Quantum efficiency

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Proceedings Article | 9 October 2003 Paper

Paper

Confocal microscopy: an experimental set up for biomolecule structure investigation based on dynamical fluorescence spectroscopy

Amanda Oldani, Maddalena Collini, Giuseppe Chirico, Laura D'Alfonso, Giancarlo Baldini, Filomena Mazzei, Flavia Barone, Luciano Cellai, Cesare Giordano, Marziale Milani, Antonio Pasinetti

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500197

KEYWORDS: Modulation, Microscopes, Phase shift keying, Luminescence, Anisotropy, Fluorescence anisotropy, Confocal microscopy, Fluorometers, Polarization, Objectives

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Proceedings Article | 10 July 2003 Paper

Paper

Thermal bleaching in single fluorescent molecules under two-photon excitation regime

Giuseppe Chirico, Fabio Cannone, Giancarlo Baldini, Alberto Diaspro

Proceedings Volume 4963, (2003) https://doi.org/10.1117/12.478031

KEYWORDS: Luminescence, Molecules, Rhodamine, Glasses, Absorption, Temperature metrology, Molecular spectroscopy, Coating, Head, Single molecule spectroscopy

Read Abstract +

SPIE Journal Paper | 1 July 2003 Open Access

Open Access

Two-photon interactions at single fluorescent molecule level

Fabio Cannone, Giuseppe Chirico, Alberto Diaspro

JBO, Vol. 8, Issue 03, (July 2003) https://doi.org/10.1117/12.10.1117/1.1582180

KEYWORDS: Luminescence, Molecules, Glasses, Molecular interactions, Microscopes, Rhodamine, Confocal microscopy, Physics, Imaging spectroscopy, Imaging systems

Proceedings Article | 19 June 2003 Paper

Paper

Response of living cells to nanostructured polyelectrolyte matrices studied by means of 1-, 2-photon excitation microscopy

Alberto Diaspro, Silke Krol, Daniela Silvano, Paola Fronte, Ornella Cavalleri, Giuseppe Chirico, Francesco Beltrame, Paola Ramoino, Alessandra Gliozzi

Proceedings Volume 4962, (2003) https://doi.org/10.1117/12.478124

KEYWORDS: Luminescence, Confocal microscopy, Yeast, Microscopy, Two photon excitation microscopy, Nanostructuring, Systems modeling, Molecules, Coating, Optical filters

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Proceedings Article | 28 May 2002 Paper

Paper

Functional imaging of living Paramecium by means of confocal and two-photon excitation fluorescence microscopy

Alberto Diaspro, Paola Fronte, Marco Raimondo, Marco Fato, Gianluca DeLeo, Francesco Beltrame, Fabio Cannone, Giberto Chirico, Paola Ramoino

Proceedings Volume 4622, (2002) https://doi.org/10.1117/12.468356

KEYWORDS: Luminescence, Confocal microscopy, Plasma, Microscopy, Optical filters, Image fusion, Second-harmonic generation, Functional imaging, Particles, Molecules

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SPIE Journal Paper | 1 July 2001 Open Access

Open Access

Two-photon microscopy and spectroscopy based on a compact confocal scanning head

Alberto Diaspro, Giberto Chirico, Federico Federici, Fabio Cannone, Sabrina Beretta, Mauro Robello, Francesca Olivini, Paola Ramoino

JBO, Vol. 6, Issue 03, (July 2001) https://doi.org/10.1117/12.10.1117/1.1382809

KEYWORDS: Luminescence, Confocal microscopy, Microscopes, Spectroscopy, Polarization, Head, Molecules, Diffusion, Physics, Imaging spectroscopy

Proceedings Article | 24 April 2001 Paper

Paper

Combined confocal and spectroscopic TPE architecture for the identification of single fluorescent molecules

Giuseppe Chirico, Fabio Cannone, Francesca Olivini, Sabrina Beretta, Giancarlo Baldini, Alberto Diaspro, Mauro Robello

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424583

KEYWORDS: Spectroscopy, Luminescence, Imaging spectroscopy, Rhodamine, Molecules, Glasses, Solids, Microscopes, Head, Objectives

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Proceedings Article | 17 June 1993 Paper

Paper

Effect of repeated insertions of curved sequences in DNA plasmids: a light-scattering study

Stefano Beretta, Giuseppe Chirico, Giancarlo Baldini, U. Kapp, G. Badaracco

Proceedings Volume 1922, (1993) https://doi.org/10.1117/12.146215

KEYWORDS: Diffusion, Dynamic light scattering, Light scattering, Optical filters, Scattering, Molecular lasers, Molecular biology, Information operations, Contamination, Temperature metrology

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Showing 5 of 33 publications

Conference Committee Involvement (4)

Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI

26 June 2023 | Munich, Germany

Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V

21 June 2021 | Online Only, Germany

Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV

24 June 2019 | Munich, Germany

Optical Methods for Inspection, Characterization, and Imaging of Biomaterials

26 June 2017 | Munich, Germany

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