The holographic performance of photo-polymeric material PQ/PMMA is found to be largely determined by pre-polymerization modulation, such as stirring time and pre-polymerization temperature, during the material preparation process. In the current study, in order to determine the best stirring time during the pre-polymerization process, the influence of stirring time on the holographic properties of PQ/PMMA here is seriously analyzed. Experimental observations clearly indicate that, under the same baking conditions, the diffraction efficiency of PQ/PMMA increase initially with the stirring time but then decrease as the stirring time continue increases. When the stirring time is 75 min, the holographic performance of PQ/PMMA reaches its best in which the diffraction efficiency of the material can reach up to 49.3%. Current study here determines the optimal stirring time and pre-polymerization temperature during the pre-polymerization process, thus provide an effective guidance for further preparation of PQ/PMMA photo-polymer materials with excellent holographic properties.