Mr. Zhiyi Yuan Profile

Zhiyi Yuan

at Nanyang Technological Univ

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Publications (2)

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SPIE Journal Paper | 28 October 2020

Dynamic photonic barcodes for molecular detection based on cavity-enhanced energy transfer

Yunke Zhou, Zhiyi Yuan, Xuerui Gong, Muhammad Birowosuto, Cuong Dang, Yu-Cheng Chen

AP Vol. 2 Issue 06

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Optical barcodes have demonstrated a great potential in multiplexed bioassays and cell tracking for their distinctive spectral fingerprints. The vast majority of optical barcodes were designed to identify a specific target by fluorescence emission spectra, without being able to characterize dynamic changes in response to analytes through time. To overcome these limitations, the concept of the bioresponsive dynamic photonic barcode was proposed by exploiting interfacial energy transfer between a microdroplet cavity and binding molecules. Whispering-gallery modes resulting from cavity-enhanced energy transfer were therefore converted into photonic barcodes to identify binding activities, in which more than trillions of distinctive barcodes could be generated by a single droplet. Dynamic spectral barcoding was achieved by a significant improvement in terms of signal-to-noise ratio upon binding to target molecules. Theoretical studies and experiments were conducted to elucidate the effect of different cavity sizes and analyte concentrations. Time-resolved fluorescence lifetime was implemented to investigate the role of radiative and non-radiative energy transfer. Finally, microdroplet photonic barcodes were employed in biodetection to exhibit great potential in fulfilling biomedical applications.

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Proceedings Article | 10 October 2020 Presentation + Paper

Electrostatic-responsive microdroplet lasers for ultrasensitive molecular detection

Ziyihui Wang, Yifan Zhang, Tianhua Xu, Zhiyi Yuan, Jiazheng Ding, Yu-Cheng Chen, Tiegen Liu

Proc. SPIE. 11554, Advanced Sensor Systems and Applications X

KEYWORDS: Optical microscopes, Biosensing, Biomedical optics, Sensors, Optical resonators, Liquid crystals, Molecular interactions, Molecular lasers, Microsensors

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Electrostatics plays a critical function in most biomolecules, therefore monitoring subtle biomolecular bindings and dynamics via the electrostatic changes of biomolecules at biointerfaces has been an attractive topic recently and has provided the basis in diagnosis and biomedical science. Here we present a bioelectrostatic responsive microlaser based on liquid crystal (LC) droplet and explored its application for ultrasensitive detection of negatively charged biomolecules. Whispering gallery mode (WGM) lasing from positively charged LC microdroplets was applied as the optical resonator, where the lasing wavelength shift was employed as a sensing parameter. With the dual impacts from whispering-gallery mode and liquid crystal, molecular binding signals will be amplified in such LC droplet sensors. It is found that molecular electrostatic changes at the biointerface of droplet triggered wavelength shift in lasing spectra. The total wavelength shift increased proportionally with the adhering target concentrations. Compared to a conventional polarized optical microscope, significant improvements in sensitivity and dynamic range by four orders of magnitude were achieved. Our work indicated that the surface-to-volume ratio plays a critical role in the detection sensitivity in WGM laser-based microsensors. Finally, bovine serum albumin and specific biosensing using streptavidin and biotin were exploited to demonstrate the potential applications of microlasers with a detection limit on the order of 1 pM. We anticipate this approach will open new possibilities for the ultrasensitive label-free detection of charged biomolecules and molecular interactions by providing a lower detection limit than conventional methods.

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