Presentation
2 March 2022 Stimulated Raman microscopy for classification of micro-fibers from environmental samples
Author Affiliations +
Abstract
The assessment of man-made or natural origin of microscopic debris in the environment requires spectroscopical analysis. However, it is difficult to use commercial FT-IR and spontaneous Raman microspectrometers to analyze micro-fibers due to their small size and strong autofluorescence in some specimens. Using a custom stimulated Raman scattering (SRS) microscope, we assessed the origin of microfibers from different environments by comparing their SRS spectra to a home-built library. Our analysis shows that the majority of the analysed micro-fibers are of natural origin. We show that SRS microscopy overcomes the limitations of FT-IR and spontaneous Raman microscopy for classification of micro-fibers.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Sergey P. Laptenok, Luca Genchi, Cecilia Martin, Fadiyah M. Baalkhuyur, Carlos M. Duarte, and Carlo Liberale "Stimulated Raman microscopy for classification of micro-fibers from environmental samples", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC1197319 (2 March 2022); https://doi.org/10.1117/12.2608165
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KEYWORDS
Raman spectroscopy

Microscopy

Chemical analysis

Spectroscopy

FT-IR spectroscopy

Polymers

Statistical analysis

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