Multimodal coherent Raman and multiphoton nonlinear microscopy reveals early therapy-induced senescence in human cancer cells to prevent tumor recurrence

Arianna Bresci; Francesco Manetti; Silvia Ghislanzoni; Federico Vernuccio; Salvatore Sorrentino; Chiara Ceconello; Renzo Vanna; Italia Bongarzone; Giulio Cerullo; Dario Polli

doi:10.1117/12.2650240

17 March 2023 Multimodal coherent Raman and multiphoton nonlinear microscopy reveals early therapy-induced senescence in human cancer cells to prevent tumor recurrence

Arianna Bresci, Francesco Manetti, Silvia Ghislanzoni, Federico Vernuccio, Salvatore Sorrentino, Chiara Ceconello, Renzo Vanna, Italia Bongarzone, Giulio Cerullo, Dario Polli

Author Affiliations +

Proceedings Volume PC12363, Multiscale Imaging and Spectroscopy IV; PC1236303 (2023) https://doi.org/10.1117/12.2650240
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Cancer research recently revealed that anticancer therapies can cause cell senescence instead of death, a phenotype governing tumor relapse. Developing safe, quick, and precise tools to spot such therapy-induced senescence (TIS) is an urgency. We present multimodal coherent Raman and multiphoton nonlinear optical microscopy as powerful to unveil TIS, via a home-built microscope including forward-detected Stimulated Raman Scattering, forward and epi-detected Coherent Anti-Stokes Raman Scattering, Two-Photon Excited Fluorescence and Second-Harmonic Generation modalities. We exposed early TIS in human cancer cells, confirmed comparing diverse signals during therapy period. We consider our findings will strongly influence anticancer practices, helping prevent tumor recurrence.

Conference Presentation

Citation Download Citation

Arianna Bresci, Francesco Manetti, Silvia Ghislanzoni, Federico Vernuccio, Salvatore Sorrentino, Chiara Ceconello, Renzo Vanna, Italia Bongarzone, Giulio Cerullo, and Dario Polli "Multimodal coherent Raman and multiphoton nonlinear microscopy reveals early therapy-induced senescence in human cancer cells to prevent tumor recurrence", Proc. SPIE PC12363, Multiscale Imaging and Spectroscopy IV, PC1236303 (17 March 2023); https://doi.org/10.1117/12.2650240

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available