Correlative imaging and quantification of the tumor microenvironment of triple-negative-breast-cancer using lightsheet microscopy, scanning laser optical tomography, and TPEF

H. Kamin; L. Nolte; J. Maurer; E. Stickeler; D. Heinemann; A. Heisterkamp; S. Johannsmeier; T. Ripken

doi:10.1117/12.2648373

6 March 2023 Correlative imaging and quantification of the tumor microenvironment of triple-negative-breast-cancer using lightsheet microscopy, scanning laser optical tomography, and TPEF

H. Kamin, L. Nolte, J. Maurer, E. Stickeler, D. Heinemann, A. Heisterkamp, S. Johannsmeier, T. Ripken

Author Affiliations +

Proceedings Volume PC12371, Multimodal Biomedical Imaging XVIII; PC1237104 (2023) https://doi.org/10.1117/12.2648373
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

The triple-negative breast cancer is an aggressive subtype that has a high rate of relapse and a poor five-year survival rate. The Tumor Microenviroment influences the behaviors such as proliferation, migration and the formation of metastasis. We present the complementary imaging and subsequent analysis of the Tumor Microenviroment using Lightsheet Microscopy, Scanning Laser Optical Tomography and TPEF. The samples were imaged in different size and resolution scales and the three-dimensional information is obtained using the SHG-signal of collagen and fluorescence of labeled key markers. The results presented here may help to create a patient specific model of breast cancer.

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H. Kamin, L. Nolte, J. Maurer, E. Stickeler, D. Heinemann, A. Heisterkamp, S. Johannsmeier, and T. Ripken "Correlative imaging and quantification of the tumor microenvironment of triple-negative-breast-cancer using lightsheet microscopy, scanning laser optical tomography, and TPEF ", Proc. SPIE PC12371, Multimodal Biomedical Imaging XVIII, PC1237104 (6 March 2023); https://doi.org/10.1117/12.2648373

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