Imaging System For Correlating Fluorescence Cell Measurements In Flow

David B. Kay; James L. Cambier; Leon L. Wheeless

doi:10.1117/12.955901

21 November 1977 Imaging System For Correlating Fluorescence Cell Measurements In Flow

David B. Kay, James L. Cambier, Leon L. Wheeless

Proceedings Volume 0126, Clever Optics: Innovative Applications of Optics; (1977) https://doi.org/10.1117/12.955901
Event: 21st Annual Technical Symposium, 1977, San Diego, United States

Abstract

A high sensitivity camera and imaging system are employed in a special purpose flow cytofluorometer for extraction of fluorescence images of cells and objects in flow. Each image is uniquely correlated with a fluorescence profile or "slit-scan" contour of the cell also derived in flow. Contours are computer analyzed to extract low resolution morphologic information. Optical considerations for imaging in flow and results obtained from the imaging system are presented.

Citation Download Citation

David B. Kay, James L. Cambier, and Leon L. Wheeless "Imaging System For Correlating Fluorescence Cell Measurements In Flow", Proc. SPIE 0126, Clever Optics: Innovative Applications of Optics, (21 November 1977); https://doi.org/10.1117/12.955901

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