Super-duper chemiluminescent proteins applicable to wide range of bioimaging

Kazushi Suzuki; Takeharu Nagai

doi:10.1117/12.2249770

8 February 2017 Super-duper chemiluminescent proteins applicable to wide range of bioimaging

Kazushi Suzuki, Takeharu Nagai

Proceedings Volume 10052, Optogenetics and Optical Manipulation; 1005202 (2017) https://doi.org/10.1117/12.2249770
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We report five new spectral variants of bright luminescent protein made by concatenation of the brightest luciferase, NanoLuc, with various color hues of fluorescent proteins. These proteins, which we call enhanced Nano-lanterns (eNLs), allow five-color live-cell imaging without external light illumination as well as detection of single molecules. Furthermore, eNL-based Ca²⁺ indicators could be used to image long-term Ca²⁺ dynamics in iPS-derived cardiomyocytes.

Citation Download Citation

Kazushi Suzuki and Takeharu Nagai "Super-duper chemiluminescent proteins applicable to wide range of bioimaging", Proc. SPIE 10052, Optogenetics and Optical Manipulation, 1005202 (8 February 2017); https://doi.org/10.1117/12.2249770

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