New approaches to intracellular drug imaging by stimulated Raman scattering microscopy

M. Lee; W. J. Tipping; A. Serrels; A. N. Hulme; V. G. Brunton

doi:10.1117/12.2253847

21 February 2017 New approaches to intracellular drug imaging by stimulated Raman scattering microscopy

M. Lee, W. J. Tipping, A. Serrels, A. N. Hulme, V. G. Brunton

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100690T (2017) https://doi.org/10.1117/12.2253847
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

A range of proposed alkyne Raman tags are examined in-silico for activity and then synthesised generating a library of analogues of the natural product, anisomycin. We report the use of bisaryl butadiyne-anisomycin (BADY-anisomycin) in intracellular SRS microscopy studies of uptake and localisation within live and fixed cells. Following rational design and synthesis, BADY-anisomycin was shown to produce an intense Raman band at 2219 cm^-1, that is centrally located within the cellular silent region and is approximately 60 times more Raman active than the corresponding propargylanisomycin. Finally, we demonstrate two-colour imaging utilising EdU, an alkyne-containing proliferation probe and BADY-anisomycin.

Conference Presentation

Citation Download Citation

M. Lee, W. J. Tipping, A. Serrels, A. N. Hulme, and V. G. Brunton "New approaches to intracellular drug imaging by stimulated Raman scattering microscopy", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690T (21 February 2017); https://doi.org/10.1117/12.2253847

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