Fabrication of 3D micro-structured scaffolds by direct laser writing in pre-polymers for in vitro and in vivo studies

Sima Rekštytė; Justinas Mačiulaitis; Romaldas Mačiulaitis; Mangirdas Malinauskas

doi:10.1117/12.2249579

17 February 2017 Fabrication of 3D micro-structured scaffolds by direct laser writing in pre-polymers for in vitro and in vivo studies

Sima Rekštytė, Justinas Mačiulaitis, Romaldas Mačiulaitis, Mangirdas Malinauskas

Author Affiliations +

Proceedings Volume 10094, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XVII; 1009403 (2017) https://doi.org/10.1117/12.2249579
Event: SPIE LASE, 2017, San Francisco, California, United States

Abstract

We present the results of fabricating micro-structured macro-constructs for tissue engineering applications by using direct laser writing in pre-polymers (DLW-PP). The limiting factors for rapid construction of millimeter sized scaffolds, including the choice of material, software and hardware, as well as geometry, are discussed. We demonstrate that by using the hybrid inorganic-organic pre-polymer SZ2080 and employing a laser system which can combine the movement of linear motion stages with scanners, the structuring throughput reaching up to 51300 μm³/s can be achieved maintaining structural elements with 15 μm resolution. This allows to construct up to 30 scaffolds overnight with measurements reaching 1515×1515×195 μm³ – big enough for a surgeon to handle – and enables serial fabrication of such structures required for statistical tissue formation studies. Also, the versatility of DLW-PP technique is demonstrated by presenting the fabricated scaffolds with different pore shapes and sizes as well as manufactured out of pure and photo-sensitized SZ2080 material.

Conference Presentation

Citation Download Citation

Sima Rekštytė, Justinas Mačiulaitis, Romaldas Mačiulaitis, and Mangirdas Malinauskas "Fabrication of 3D micro-structured scaffolds by direct laser writing in pre-polymers for in vitro and in vivo studies", Proc. SPIE 10094, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XVII, 1009403 (17 February 2017); https://doi.org/10.1117/12.2249579

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available