Photoacoustic imaging for assessing ischemic kidney damage in vivo

Elizabeth S. L. Berndl; Xiaolin He; Darren A. Yuen; Michael C. Kolios

doi:10.1117/12.2291231

19 February 2018 Photoacoustic imaging for assessing ischemic kidney damage in vivo

Elizabeth S. L. Berndl, Xiaolin He, Darren A. Yuen, Michael C. Kolios

Proceedings Volume 10494, Photons Plus Ultrasound: Imaging and Sensing 2018; 104941P (2018) https://doi.org/10.1117/12.2291231
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Ischemic reperfusion injuries (IRIs) occur after blood returns to a tissue or organ after a period without oxygen or nutrients, which causes an inflammatory response leading to heterogeneous scarring of the nearby tissue and vasculature. This is associated with long-term decreases blood flow, and necrosis. Although most commonly associated with heart attacks and strokes, IRIs are also a side effect of organ transplants, when the organ is reperfused in the recipient’s body after being transported from the donor to the transplant hospital. Currently, the optimal method of monitoring for IRI is limited to biopsies, which are invasive and poorly monitor the spatial heterogeneity of the damage. To non-invasively identify changes in kidneys, the left renal artery in mice (n=3) was clamped for 45 minutes to create an IRI event. Both kidneys of each animal were monitored using photoacoustics (PA) with the VevoLAZR system (Fujifilm-VisualSonics, Toronto) three, four and eight weeks after surgery. IRI-treated kidneys show increased picosirius red staining, indicative of collagen (0.601 vs 0.042, p < 0.0001), decreased size as assessed by cross-sectional area (7.8 mm² vs 35.9 mm² , p < 0.0001), and decreased oxygen saturation (sO2; 62% vs 77%, p = 0.02). Analysis of the photoacoustic data shows that a two-point metric, the 715:930 nm ratio of the whole kidney (1.05 vs 0.57, p = 0.049) and the optical spectral slope (OSS) (0.8 * 10^-3 vs 3.0 * 10^-3, p = 0.013) are both able to differentiate between IRI-treated and healthy kidneys. These data suggest that photoacoustics can be used as a non-invasive method to observe in vivo changes in the kidney due to IRI.

Conference Presentation

Citation Download Citation

Elizabeth S. L. Berndl, Xiaolin He, Darren A. Yuen, and Michael C. Kolios "Photoacoustic imaging for assessing ischemic kidney damage in vivo", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104941P (19 February 2018); https://doi.org/10.1117/12.2291231

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available