Assessing tissues' inhomogeneous optical properties based on quantitative phase imaging and dark field microscopy with LED illumination (Conference Presentation)

Qiulan Liu; Cuifang Kuang; Xu Liu

doi:10.1117/12.2293608

15 March 2018 Assessing tissues' inhomogeneous optical properties based on quantitative phase imaging and dark field microscopy with LED illumination (Conference Presentation)

Qiulan Liu, Cuifang Kuang, Xu Liu

Author Affiliations +

Proceedings Volume 10503, Quantitative Phase Imaging IV; 1050315 (2018) https://doi.org/10.1117/12.2293608
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Assessing tissues’ inhomogeneous optical properties is helpful for label-free diagnosis. In this paper, a method and a setup are proposed to assess the tissues’ inhomogeneous optical properties based on quantitative phase imaging (QPI) and dark-field microscopy using LED illumination. In the bright-field and coherent dark-field imaging, quantitative phase (QP) can be recovered from multi-frame intensity images captured at different axial positions by Transport-intensity-equation (TIE) and multi-height Gerchberg-Saxton(G-S) phase retrieval algorithms. In the incoherent dark-field imaging, some scattering samples can be observed easily without any computation and the resolution can be improved in contrast to bright-field illumination. According to the scattering-phase theorem and the statistical dispersion relation (SDR), tissues’ inhomogeneous optical properties can be assessed. In our experiments, we find, it is a good idea to combine QP maps and dark-field images to assess inhomogeneous optical properties of bio-samples. In contrast to the DHM and interferometers, our setup is cost-effective and use-flexible, thus having the potential to serve as a very powerful tool in biological applications.

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Qiulan Liu, Cuifang Kuang, and Xu Liu "Assessing tissues' inhomogeneous optical properties based on quantitative phase imaging and dark field microscopy with LED illumination (Conference Presentation)", Proc. SPIE 10503, Quantitative Phase Imaging IV, 1050315 (15 March 2018); https://doi.org/10.1117/12.2293608

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KEYWORDS

Image resolution

Microscopy

Absorption

Image quality

Light scattering

Light sources

Luminescence

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