White-light common-path digital holographic microscopy for quantitative phase imaging of living cells

Lihong Ma; Jiaheng Zhang; Yong Li

doi:10.1117/12.2501094

24 July 2018 White-light common-path digital holographic microscopy for quantitative phase imaging of living cells

Lihong Ma, Jiaheng Zhang, Yong Li

Proceedings Volume 10827, Sixth International Conference on Optical and Photonic Engineering (icOPEN 2018); 108270S (2018) https://doi.org/10.1117/12.2501094
Event: Sixth International Conference on Optical and Photonic Engineering (icOPEN 2018), 2018, Shanghai, China

Abstract

In the study of the morphology and dynamic physiological characteristics of living cells, label-free quantitative phase imaging has been the most ideal detection technique. In this paper, we set up a set of white-light common-path digital holographic microscope based on grating diffraction, which can acquire high-resolution quantitative phase image with a very high spatiotemporal imaging sensitivity. Living red blood cells are measured and the accurate quantitative phase images are achieved. Experimental results demonstrate that this system has a very high accurate imaging performance and has the ability to detect living cells.

Citation Download Citation

Lihong Ma, Jiaheng Zhang, and Yong Li "White-light common-path digital holographic microscopy for quantitative phase imaging of living cells", Proc. SPIE 10827, Sixth International Conference on Optical and Photonic Engineering (icOPEN 2018), 108270S (24 July 2018); https://doi.org/10.1117/12.2501094

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