A bimodal bioluminescent Ca2+ indicator toward spatiotemporally scalable imaging

Israt Farhana; Kazushi Suzuki; Tomoki Matsuda; Takeharu Nagai

doi:10.1117/12.2518178

22 February 2019 A bimodal bioluminescent Ca²⁺ indicator toward spatiotemporally-scalable imaging

Israt Farhana, Kazushi Suzuki, Tomoki Matsuda, Takeharu Nagai

Proceedings Volume 10866, Optogenetics and Optical Manipulation 2019; 1086603 (2019) https://doi.org/10.1117/12.2518178
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

For decades, genetically encoded Ca²⁺ indicators (GECIs), have been under development for the purpose of visualizing intracellular Ca²⁺ dynamics. Fluorescent GECIs are limited for the purpose of deep tissue long-term whole-body imaging due to their requirement for external illumination. Bioluminescent GECIs overcome these restraints but are somewhat compromised in terms of spatiotemporal resolution compared to fluorescent probes. To address this, we developed a bimodal Ca²⁺ indicator by combining a single fluorescent protein based Ca²⁺ indicator and a split luciferase. The novel design of this bimodal indicator enables Ca²⁺ imaging in the same specimen in both fluorescent and bioluminescent mode. The ability to switch between fluorescent and bioluminescent modes with a single indicator should benefit applications where micro and macro scale observation of cells or tissues is desirable. Use of such a probe enables trans-scale imaging, where macro scale imaging of a group of cells or tissue can be combined with fluorescent imaging of single cells.

Citation Download Citation

Israt Farhana, Kazushi Suzuki, Tomoki Matsuda, and Takeharu Nagai "A bimodal bioluminescent Ca²⁺ indicator toward spatiotemporally-scalable imaging", Proc. SPIE 10866, Optogenetics and Optical Manipulation 2019, 1086603 (22 February 2019); https://doi.org/10.1117/12.2518178

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