Characterization of collagen formation surrounding osteocytes using second and third harmonic generation

Emily G. Pendleton; Kayvan F. Tehrani; Ruth P. Barrow; Luke J. Mortensen

doi:10.1117/12.2510814

27 February 2019 Characterization of collagen formation surrounding osteocytes using second and third harmonic generation

Emily G. Pendleton, Kayvan F. Tehrani, Ruth P. Barrow, Luke J. Mortensen

Author Affiliations +

Proceedings Volume 10871, Multimodal Biomedical Imaging XIV; 108710W (2019) https://doi.org/10.1117/12.2510814
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

More than 54 million Americans have or are at high risk of developing a metabolic bone disease; disorders of bone strength that leave individuals with fragile bones and disabilities. The gold standard to evaluate these diseases is dual energy x-ray absorptiometry, but this only measures mineral content. These diseases, however, impact collagen and mineral integrity which impede the bone’s ability to store hormones, proteoglycans, and glycoproteins imperative to homeostasis. We have established a second harmonic generation (SHG) polarimetric assay that describes bone collagen organization. To further our analysis, we propose multimodal optical evaluation of bone quality with third harmonic generation (THG) to measure osteocyte dendritic processes. This method of analysis could be used to evaluate the disease state of bone and response to therapy.

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Emily G. Pendleton, Kayvan F. Tehrani, Ruth P. Barrow, and Luke J. Mortensen "Characterization of collagen formation surrounding osteocytes using second and third harmonic generation", Proc. SPIE 10871, Multimodal Biomedical Imaging XIV, 108710W (27 February 2019); https://doi.org/10.1117/12.2510814

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