Cell-surface affinity sensors for identifying and selecting highly cytokine-secreting cells  (Conference Presentation)

Ewa M. Goldys; Guozhen Liu; Ayad G. Anwer; Siân P. Cartland; Kaixin Zhang; Michael A. Cahill; Lindsay M. Parker; Shilun Feng; Meng He; David W. Inglis; Nicolle H. Packer; Mary M. Kavurma; Mark R. Hutchinson; Christina Bursill

doi:10.1117/12.2508971

4 March 2019 Cell-surface affinity sensors for identifying and selecting highly cytokine-secreting cells (Conference Presentation)

Ewa M. Goldys, Guozhen Liu, Ayad G. Anwer, Siân P. Cartland, Kaixin Zhang, Michael A. Cahill, Lindsay M. Parker, Shilun Feng, Meng He, David W. Inglis, Nicolle H. Packer, Mary M. Kavurma, Mark R. Hutchinson, Christina Bursill

Author Affiliations +

Proceedings Volume 10891, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI; 108910H (2019) https://doi.org/10.1117/12.2508971
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Cytokines play critical roles in homeostatic control of health and they are integral for the creation and maintenance of a myriad of disease states. Their ultra-low concentration, often in the picomolar range, and extremely dynamic transient secretion process place stringent demands on cytokine quantification. We developed a nanoparticle-based strategy to detect trace cytokine secretion from individual, single live cells, for which we coined the term “OnCELISA”. Using a capture surface on the cell membrane and fluorescent magnetic nanoparticles as assay reporters, our universal OnCELISA assay achieved the sensitivity 0.1 pg mL-1, an over 10-fold enhancement, compared to state-of-the-art. The sensitive OnCELISA cell labelling made it possible to select and sort different cell types to determine highly cytokine - secreting cell subpopulations . The capture surfaces on cell membranes did not show noticeable effect on cell viability and their subsequent proliferation. The capability to specifically select such highly cytokine-secreting cells and purify their populations is pivotal for their use in multicellular pathologies such as atherosclerosis. Accordingly, we used this new approach to label cytokine secretion from vascular tissues of apolipoprotein E-/- mice; an in vivo model of atherosclerosis. In response to lipopolysaccharide, we observed increased capture of cytokine using this model. With the capacity of monitoring multiple cytokine secretions (IL-6 and IL-1β)), our OnCELISA method is able to probe how the individual cells and tissues secrete cytokines as they respond in real time to the surrounding signals.

Conference Presentation

Citation Download Citation

Ewa M. Goldys, Guozhen Liu, Ayad G. Anwer, Siân P. Cartland, Kaixin Zhang, Michael A. Cahill, Lindsay M. Parker, Shilun Feng, Meng He, David W. Inglis, Nicolle H. Packer, Mary M. Kavurma, Mark R. Hutchinson, and Christina Bursill "Cell-surface affinity sensors for identifying and selecting highly cytokine-secreting cells (Conference Presentation)", Proc. SPIE 10891, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI, 108910H (4 March 2019); https://doi.org/10.1117/12.2508971

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Sensors

Tissues

Magnetism

Nanoparticles

Pathology

Keywords/Phrases

Search In:

Publication Years