Imaging mitochondrial matrix viscosity in live cells via fluorescence lifetime imaging (FLIM) of fluorescent molecular rotors

I. Emilie Steinmark; Arjuna L. James; Cécile A. Dreiss; Gokhan Yahioglu; Klaus Suhling

doi:10.1117/12.2508676

7 March 2019 Imaging mitochondrial matrix viscosity in live cells via fluorescence lifetime imaging (FLIM) of fluorescent molecular rotors

I. Emilie Steinmark, Arjuna L. James, Cécile A. Dreiss, Gokhan Yahioglu, Klaus Suhling

Author Affiliations +

Proceedings Volume 10893, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI; 108930K (2019) https://doi.org/10.1117/12.2508676
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Here we use the combination of fluorescent molecular rotors (FMR) and fluorescence lifetime imaging (FLIM) to image matrix viscosity in live cells. We use non-cancerous, epithelial human cornea (HCE) cells as a model system. We find that mitochondrial matrix viscosity varies between individual cells and even between individual organelles, showcasing the potential of viscosity imaging for cell biology purposes.

Conference Presentation

Citation Download Citation

I. Emilie Steinmark, Arjuna L. James, Cécile A. Dreiss, Gokhan Yahioglu, and Klaus Suhling "Imaging mitochondrial matrix viscosity in live cells via fluorescence lifetime imaging (FLIM) of fluorescent molecular rotors", Proc. SPIE 10893, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI, 108930K (7 March 2019); https://doi.org/10.1117/12.2508676

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