Additive manufacturing of catalytically active living material hydrogels

Trevor G. Johnston; Christopher R. Fellin; Alberto Carignano; Alshakim Nelson

doi:10.1117/12.2518653

13 May 2019 Additive manufacturing of catalytically active living material hydrogels

Trevor G. Johnston, Christopher R. Fellin, Alberto Carignano, Alshakim Nelson

Proceedings Volume 10982, Micro- and Nanotechnology Sensors, Systems, and Applications XI; 109820B (2019) https://doi.org/10.1117/12.2518653
Event: SPIE Defense + Commercial Sensing, 2019, Baltimore, MD, United States

Abstract

Herein, we describe a yeast-laden hydrogel ink that can be printed using a direct-write 3D printer and used for the production of a peptide. A poly(alkyl glycidyl ether)-based triblock copolymer was synthesized and formulated as a hydrogel that was characterized via rheometry to evaluate the printability of the hydrogel ink. An engineered yeast strain with an upregulated α-factor production pathway was incorporated into the hydrogel ink and 3D printed. The immobilized yeast cells exhibited adequate viability of 87.5% within the hydrogel. The production of the up-regulated α- factor was detected using a detecting yeast strain and quantified at 268 nM (s = 34.6 nM) over 72 h. The reusability of these systems was demonstrated by immersion of the yeast-laden hydrogel lattice in fresh SC media and confirmed by the detection of similar amounts of up-regulated α-factor 259 nM (s = 45.1 nM). These yeast-laden materials represent an attractive opportunity for whole-cell catalysis of other high-value products in a sustainable and continuous manner.

Conference Presentation

Citation Download Citation

Trevor G. Johnston, Christopher R. Fellin, Alberto Carignano, and Alshakim Nelson "Additive manufacturing of catalytically active living material hydrogels", Proc. SPIE 10982, Micro- and Nanotechnology Sensors, Systems, and Applications XI, 109820B (13 May 2019); https://doi.org/10.1117/12.2518653

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