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22 July 2019 Activity of smooth muscle cells after short-term heating/stretch-fixing up to 96 hours
Nao Kaminota, Emiyu Ogawa, Tsunenori Arai
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Proceedings Volume 11075, Novel Biophotonics Techniques and Applications V; 110751L (2019) https://doi.org/10.1117/12.2527059
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany
Abstract
We studied the influence of short-term heating/stretch-fixing stimulus on smooth muscle cells in vitro to determine the condition of optimum vascular dilatation for our novel angioplasty. A short-term thermal angioplasty named Photo-Thermo Dynamic Balloon (PTDB) has been proposed for stenotic artery treatment. Stretch-fixed smooth muscle cells after PTDB dilatation was reported previously. This formation would be a risk of restenosis because of enhancement in smooth muscle cells’ migration ability. To estimate migration ability after short-term heating/stretch-fixing, we studied respiratory activity of smooth muscle cells up to 96 hours in vitro. Smooth muscle cells were heated (50ºC or 60ºC, 15 s) with stretching (0 or 40%), and fixed. We measured respiratory activity of smooth muscle cells and alive cell number at 48 and 96 hours after short-term heating/stretch-fixing. A WST-8 assay solution and nuclear stainings (Hoechst 33342 and Propidium Iodide) were used for measurement of the respiratory activity and alive cell number, respectively. The absorbance value at 450 nm after WST-8 assay divided by the alive cell number was used for activity evaluation. We obtained that the respiratory activity of smooth muscle cells after short-term heating/stretch-fixing was drastically decreased in the case of 60ºC heating. In the case of 50ºC heating, the respiratory activity of smooth muscle cells was decreased more gradually than that of 60ºC. We think that heating smooth muscle cells at 60ºC in PTDB dilatation might sufficiently suppress the cell migration. Chronic restenosis suppression might be suggested in that case.
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Nao Kaminota, Emiyu Ogawa, and Tsunenori Arai "Activity of smooth muscle cells after short-term heating/stretch-fixing up to 96 hours", Proc. SPIE 11075, Novel Biophotonics Techniques and Applications V, 110751L (22 July 2019); https://doi.org/10.1117/12.2527059
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KEYWORDS
Arteries
Injuries
Absorbance
Collagen
In vitro testing
Health sciences
Temperature metrology
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