Comparative analysis of smoothing filters in confocal microscopy images

Manuel G. Forero; Reynel Peña-Ambrosio; Jaime Sánchez-Tarquino; Camilo Restrepo-Taborda; Diana Rojas-Rodríguez

doi:10.1117/12.2528755

6 September 2019 Comparative analysis of smoothing filters in confocal microscopy images

Manuel G. Forero, Reynel Peña-Ambrosio, Jaime Sánchez-Tarquino, Camilo Restrepo-Taborda, Diana Rojas-Rodríguez

Proceedings Volume 11137, Applications of Digital Image Processing XLII; 1113709 (2019) https://doi.org/10.1117/12.2528755
Event: SPIE Optical Engineering + Applications, 2019, San Diego, California, United States

Abstract

Confocal microscopy is a widely used tool in the biomedical area, allowing to obtain 3D images with a high spatial resolution. Despite having advantages over conventional microscopy, the analysis of cellular images through confocal microscopy is a complicated process due to the very low S/N, so the use of filters is necessary to reduce noise. However, this step normally affects the quality of the edges, making them more diffuse. In addition, images acquired in confocal microscopy are affected by distortions introduced by lenses and the acquisition system. Therefore, it is possible to improve the edges definition by eliminating distortions. This is done by means of deconvolution methods such as the Wiener filter. Furthermore, in recent years a new generation of smoothing filters have been developed that seek to reduce Gaussian-type noise, without losing edges. However, a study has not been carried out to determine if these filters can be used in the elimination of noise in confocal microscopy, which are contaminated with Poisson noise. Therefore, in this work we present a comparative study of ten filters for the elimination of noise in confocal microscopy: median, anisotropic diffusion, bilateral, propagated, improved propagated, Rudin-Osher-Fatemi (ROF), TVL1, non-local means, K-SVD, and Wavelet 'A trous' and Haar filters, with and without preprocessing images with the Wiener filter, taking as criteria the noise reduction and the conservation of edges.

Conference Presentation

Citation Download Citation

Manuel G. Forero, Reynel Peña-Ambrosio, Jaime Sánchez-Tarquino, Camilo Restrepo-Taborda, and Diana Rojas-Rodríguez "Comparative analysis of smoothing filters in confocal microscopy images", Proc. SPIE 11137, Applications of Digital Image Processing XLII, 1113709 (6 September 2019); https://doi.org/10.1117/12.2528755

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available