Nanosecond scanned x-ray sheet imaging for time-resolved luminescence tomography

Arthur Pétusseau; Petr Bruza; Brian W. Pogue

doi:10.1117/12.2544438

25 February 2020 Nanosecond scanned x-ray sheet imaging for time-resolved luminescence tomography

Arthur Pétusseau, Petr Bruza, Brian W. Pogue

Proceedings Volume 11224, Optics and Ionizing Radiation; 112240C (2020) https://doi.org/10.1117/12.2544438
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Biomedical imaging techniques are often limited by the loss of resolution with depth due to light scattering in biological tissue. Beyond a few millimeters in depth, diffuse transport dominates and makes high resolution imaging impossible using conventional techniques. In this work, light sheet imaging using x-ray photons was developed with a keV x-ray source. This partially overcomes this scattering by generating light within tissue at depth. The light excites fluorescent probes that can be used for tumor tracking based upon molecular targeting. Most of the fluorescent probes have a lifetime in the nanosecond range. In this study, the use of a portable linear accelerator delivering 30-ns x-ray pulses was explored. Using x-ray excitable fluorophores, light was generated within a tissue phantom. Image stacks were acquired using an intensified camera (PiMAX4 – Princeton Instrument – USA) placed perpendicularly to the slicing direction of the sample. A solid-state silicon photomultiplier was used to gate acquisition. Although this delayed acquisition slightly, it improved the fluorescence signal-to-noise ratio (SNR). A deconvolution algorithm counteracted the blurring effects of tissue, and image stacks were converted to 3D reconstructions. In summary, nanosecond x-ray pulses can be used to excite fluorophores through radioluminescence phenomenon. Combined with slice imaging, this approach shows promise for time-resolved x-ray luminescence.

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Arthur Pétusseau, Petr Bruza, and Brian W. Pogue "Nanosecond scanned x-ray sheet imaging for time-resolved luminescence tomography", Proc. SPIE 11224, Optics and Ionizing Radiation, 112240C (25 February 2020); https://doi.org/10.1117/12.2544438

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KEYWORDS

X-rays

Luminescence

X-ray sources

X-ray imaging

Beam shaping

Photons

Biomedical optics

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