G-protein coupled receptor signaling probed by a microtoroid-proteolipid system (Conference Presentation)

Phuong Diem Nguyen; Adley Gin; Cheng Li; Judith Su

doi:10.1117/12.2545495

9 March 2020 G-protein coupled receptor signaling probed by a microtoroid-proteolipid system (Conference Presentation)

Phuong Diem Nguyen, Adley Gin, Cheng Li, Judith Su

Proceedings Volume 11233, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XX; 112330M (2020) https://doi.org/10.1117/12.2545495
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Most biosensors rely on immobilized antibodies or aptamers. In contrast, receptor proteins exist naturally in lipid bilayers and are highly specific to small molecules. We use a frequency-locked optical whispering evanescent resonator (FLOWER) system for real-time quantification of rhodopsin incorporation into an artificial lipid membrane and observe photo-induced molecular transformations upon light activation. Our study of proteolipid membrane coated microtoroids for probing the local activity of G-protein coupled receptors was further expanded to kappa-opioid receptors and their endogenous ligand Dynorphin-A. G-protein coupled receptor signaling probed by a microtoroid-proteolipid system will facilitate drug discovery and therapeutic interventions.

Conference Presentation

Citation Download Citation

Phuong Diem Nguyen, Adley Gin, Cheng Li, and Judith Su "G-protein coupled receptor signaling probed by a microtoroid-proteolipid system (Conference Presentation)", Proc. SPIE 11233, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XX, 112330M (9 March 2020); https://doi.org/10.1117/12.2545495

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